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Conformational analysis and interaction of the Staphylococcus aureus transmembrane peptidase AgrB with its AgrD propeptide substrate (2023)
Journal Article
Bardelang, P., Murray, E. J., Blower, I., Zandomeneghi, S., Goode, A., Hussain, R., …Bonev, B. B. (2023). Conformational analysis and interaction of the Staphylococcus aureus transmembrane peptidase AgrB with its AgrD propeptide substrate. Frontiers in Chemistry, 11, Article 1113885. https://doi.org/10.3389/fchem.2023.1113885

Virulence gene expression in the human pathogen, S. aureus is regulated by the agr (accessory gene regulator) quorum sensing (QS) system which is conserved in diverse Gram-positive bacteria. The agr QS signal molecule is an autoinducing peptide (AIP)... Read More about Conformational analysis and interaction of the Staphylococcus aureus transmembrane peptidase AgrB with its AgrD propeptide substrate.

Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus (2020)
Journal Article
Blower, I., Tong, C., Sun, X., Murray, E., Luckett, J., Chan, W., …Hill, P. (2020). Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus. Sensors, 20(15), 1-12. https://doi.org/10.3390/s20154305

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Gaussia luciferase (GLuc) is a secreted protein with significant potential for use as a reporter of gene expression in bacterial pathogenicity studies. To date there are relatively few example... Read More about Gaussia luciferase as a reporter for quorum sensing in staphylococcus aureus.

Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions (2020)
Journal Article
Grigoriou, S., Kugler, P., Kulcinskaja, E., Walter, F., King, J., Hill, P., …O'Reilly, E. (2020). Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions. Green Chemistry, 22(13), 4128-4132. https://doi.org/10.1039/d0gc01432j

The development of biocatalytic routes for the synthesis of chiral amines starting from achiral building blocks is highly desirable. Here, we report a self-sufficient whole-cell system for the conversion of a model ketone to the corresponding cyclic... Read More about Development of a Corynebacterium glutamicum bio-factory for self-sufficient transaminase reactions.

Ultrasound-mediation of self-illuminating reporters improves imaging resolution in optically scattering media (2018)
Journal Article
Ahmad, J., Jayet, B., Hill, P. J., Mather, M. L., Dehghani, H., & Morgan, S. P. (in press). Ultrasound-mediation of self-illuminating reporters improves imaging resolution in optically scattering media. Biomedical Optics Express, 9(4), https://doi.org/10.1364/BOE.9.001664

In vivo imaging of self-illuminating bio-and chemiluminescent reporters is used to observe the physiology of small animals. However, strong light scattering by biological tissues results in poor spatial resolution of the optical imaging, which also d... Read More about Ultrasound-mediation of self-illuminating reporters improves imaging resolution in optically scattering media.

Reconstructing promoter activity from Lux bioluminescent reporters (2017)
Journal Article
Iqbal, M., Doherty, N., Page, A. M., Qazi, S. N., Ajmera, I., Lund, P. A., …Stekel, D. J. (2017). Reconstructing promoter activity from Lux bioluminescent reporters. PLoS Computational Biology, 13(9), Article e1005731. https://doi.org/10.1371/journal.pcbi.1005731

The bacterial Lux system is used as a gene expression reporter. It is fast, sensitive and non-destructive, enabling high frequency measurements. Originally developed for bacterial cells, it has also been adapted for eukaryotic cells, and can be used... Read More about Reconstructing promoter activity from Lux bioluminescent reporters.

Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain (2017)
Journal Article
Lewis, J. E., Brameld, J. M., Hill, P., Cocco, C., Noli, B., Ferri, G., …Jethwa, P. (2017). Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain. PLoS ONE, 12, Article e01727. https://doi.org/10.1371/journal.pone.0172724

VGF (non-acronymic) was first highlighted to have a role in energy homeostasis through experiments involving dietary manipulation in mice. Fasting increased VGF mRNA in the Arc and levels were subsequently reduced upon refeeding. This anabolic role f... Read More about Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain.