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All Outputs (9)

Antigen array for serological diagnosis and novel allergen identification in severe equine asthma (2019)
Journal Article
White, S. J., Moore-Colyer, M., Marti, E., Hannant, D., Gerber, V., Coüetil, L., …Alcocer, M. (2019). Antigen array for serological diagnosis and novel allergen identification in severe equine asthma. Scientific Reports, 9, Article 15170. https://doi.org/10.1038/s41598-019-51820-7

Severe equine asthma (sEA), which closely resembles human asthma, is a debilitating and performance-limiting allergic respiratory disorder which affects 14% of horses in the Northern Hemisphere and is associated with increased allergen-specific immun... Read More about Antigen array for serological diagnosis and novel allergen identification in severe equine asthma.

Microarray Feature Selection and Dynamic Selection of Classifiers for Early Detection of Insect Bite Hypersensitivity in Horses (2019)
Conference Proceeding
Maciel Guerra, A., Figueredo, G. P., Von Zuben, F., Marti, E., Twycross, J., & Alcocer, M. J. (2019). Microarray Feature Selection and Dynamic Selection of Classifiers for Early Detection of Insect Bite Hypersensitivity in Horses. In 2019 IEEE Congress on Evolutionary Computation (CEC) (1157-1164). https://doi.org/10.1109/CEC.2019.8790319

Microarrays can be employed to better characterise allergies, as interactions between antibodies and allergens in mammals can be monitored. Once the joint dynamics of these elements in both healthy and diseased animals are understood, a model to pred... Read More about Microarray Feature Selection and Dynamic Selection of Classifiers for Early Detection of Insect Bite Hypersensitivity in Horses.

Multimeric recombinant antibody (scFv) for ELISA detection of allergenic walnut: an alternative to animal antibodies (2018)
Journal Article
Madrid, R., de la Cruz, S., García-García, A., Alcocer, M. J., González, I., García, T., & Martín, R. (2018). Multimeric recombinant antibody (scFv) for ELISA detection of allergenic walnut: an alternative to animal antibodies. Journal of Food Composition and Analysis, 67, https://doi.org/10.1016/j.jfca.2018.01.017

Walnuts are classified as an important allergenic ingredient that can cause severe reactions in sensitized individuals. To prevent unintended exposure to products containing walnut, food manufacturers have the responsibility to declare its presence i... Read More about Multimeric recombinant antibody (scFv) for ELISA detection of allergenic walnut: an alternative to animal antibodies.

Eucheuma cottonii sulfated oligosaccharides decrease food allergic responses in animal models by up-regulating regulatory T (Treg) cells (2017)
Journal Article
Xu, S., Liu, Q., Xiao, A., Maleki, S. J., Alcocer, M., Gao, Y., …Liu, G. (2017). Eucheuma cottonii sulfated oligosaccharides decrease food allergic responses in animal models by up-regulating regulatory T (Treg) cells. Journal of Agricultural and Food Chemistry, 65(15), https://doi.org/10.1021/acs.jafc.7b00389

In the present study, the anti-food allergy activity of Eucheuma cottonii sulfated oligosaccharide (ESO) was investigated. ESO was obtained by enzymatic degradation and purified by column chromatography. RBL-2H3 cells and BALB/c mouse model were used... Read More about Eucheuma cottonii sulfated oligosaccharides decrease food allergic responses in animal models by up-regulating regulatory T (Treg) cells.

Allergenicity and oral tolerance of enzymatic cross-linked tropomyosin evaluated using cell and mouse models (2017)
Journal Article
Liu, G., Hu, M., Sun, L., Han, X., Liu, Q., Alcocer, M. J., …Liu, G. (in press). Allergenicity and oral tolerance of enzymatic cross-linked tropomyosin evaluated using cell and mouse models. Journal of Agricultural and Food Chemistry, 65(10), https://doi.org/10.1021/acs.jafc.6b05816

The enzymatic cross-linking of proteins to form high-molecular-weight compounds may alter their sensitization potential. The IgG-/IgE-binding activity, digestibility, allergenicity, and oral tolerance of cross-linked tropomyosin with tyrosinase (CTC)... Read More about Allergenicity and oral tolerance of enzymatic cross-linked tropomyosin evaluated using cell and mouse models.

Recent advances in food allergy (2016)
Journal Article
Alcocer, M. J. C., Ares, S. D. L. C., & López-Calleja, I. (in press). Recent advances in food allergy. Brazilian Journal of Food Technology, 19, Article e2016047. https://doi.org/10.1590/1981-6723.4716

Food allergy is a public health issue that has significantly increased worldwide in the past decade, affecting consumers’ quality of life and making increasing demands on health service resources. Despite recent advances in many areas of diagnosis an... Read More about Recent advances in food allergy.

Anti-Food allergic activity of sulfated polysaccharide from Gracilaria lemaneiformisis dependent in immunosuppression and inhibition of p38 MAPK (2016)
Journal Article
Liu, Q., Yang, Y., Maleki, S. J., Alcocer, M., Xu, S., Shi, C., …Liu, G. (in press). Anti-Food allergic activity of sulfated polysaccharide from Gracilaria lemaneiformisis dependent in immunosuppression and inhibition of p38 MAPK. Journal of Agricultural and Food Chemistry, 64(22), https://doi.org/10.1021/acs.jafc.6b01086

Polysaccharides from Gracilaria lemaneiformis in particular possess various bioactive functions, but their antiallergic activity remains incompletely defined. Sulfated polysaccharide from Gracilaria lemaneiformis (GLSP) was obtained by water extracti... Read More about Anti-Food allergic activity of sulfated polysaccharide from Gracilaria lemaneiformisis dependent in immunosuppression and inhibition of p38 MAPK.

Production of in vivo biotinylated scFv specific to almond (Prunus dulcis) proteins by recombinant Pichia pastoris (2016)
Journal Article
de la Cruz, S., Alcocer, M., Madrid, R., García, A., Martín, R., González, I., & García, T. (2016). Production of in vivo biotinylated scFv specific to almond (Prunus dulcis) proteins by recombinant Pichia pastoris. Journal of Biotechnology, 227, https://doi.org/10.1016/j.jbiotec.2016.04.024

The methylotropic yeast Pichia pastoris has demonstrated its suitability for large-scale production of recombinant proteins. As an eukaryotic organism P. pastoris presents a series of advantages at expression and processing of heterologous proteins w... Read More about Production of in vivo biotinylated scFv specific to almond (Prunus dulcis) proteins by recombinant Pichia pastoris.

Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity (2015)
Journal Article
Marti, E., Wang, X., Jambari, N., Rhyner, C., Olzhausen, J., Pérez-Barea, J., …Alcocer, M. J. (2015). Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity. Veterinary Immunology and Immunopathology, 167(3-4), https://doi.org/10.1016/j.vetimm.2015.06.013

Insect bite hypersensitivity (IBH) is a seasonal recurrent skin allergy of horses caused by IgE-mediated reactions to allergens present in the saliva of biting insects of the genus Culicoides, and possibly also Simulium and Stomoxys species. In this... Read More about Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity.