All Outputs (37)

Protocol for the New Medicine Service Study: a randomized controlled trial and economic evaluation with qualitative appraisal comparing the effectiveness and cost effectiveness of the New Medicine Service in community pharmacies in England (2013)
Journal Article
Boyd, M., Waring, J., Barber, N., Mehta, R., Chuter, A., Avery, A., …Elliott, R. A. (2013). Protocol for the New Medicine Service Study: a randomized controlled trial and economic evaluation with qualitative appraisal comparing the effectiveness and cost effectiveness of the New Medicine Service in community pharmacies in England. Trials, 14(1), 411. https://doi.org/10.1186/1745-6215-14-411

Background Medication non-adherence is considered an important cause of morbidity and mortality in primary care. This study aims to determine the effectiveness, cost effectiveness and acceptability of a complex intervention delivered by community... Read More about Protocol for the New Medicine Service Study: a randomized controlled trial and economic evaluation with qualitative appraisal comparing the effectiveness and cost effectiveness of the New Medicine Service in community pharmacies in England.

A novel Cdk9 inhibitor preferentially targets tumor cells and synergizes with fludarabine (2013)
Journal Article
Walsby, E., Pratt, G., Shao, H., Abbas, A. Y., Fischer, P., Bradshaw, T. D., …Pepper, C. (2013). A novel Cdk9 inhibitor preferentially targets tumor cells and synergizes with fludarabine. Oncotarget, 5, 375-385

Cdk9 is a key elongation factor for RNA transcription and functions by phosphorylating the C-terminal domain of RNA polymerase II. Here we present direct evidence that cdk9 is important for cancer cell survival and describe the characterization of th... Read More about A novel Cdk9 inhibitor preferentially targets tumor cells and synergizes with fludarabine.

Apoferritin-encapsulated PbS quantum dots significantly inhibit growth of colorectal carcinoma cells (2013)
Journal Article
Bradshaw, T. D., Junor, M., Patanè, A., Clarke, P., Thomas, N. R., Li, M., …Turyanska, L. (2013). Apoferritin-encapsulated PbS quantum dots significantly inhibit growth of colorectal carcinoma cells. Journal of Materials Chemistry B, 45, 6254-6260. https://doi.org/10.1039/c3tb21197e

Colorectal carcinoma (CRC) is the 3rd most common cancer worldwide, thus development of novel therapeutic strategies is imperative. Herein potent, selective dose-dependent antitumor activity of horse spleen apoferritin encapsulated PbS quantum dots (... Read More about Apoferritin-encapsulated PbS quantum dots significantly inhibit growth of colorectal carcinoma cells.

Modelling and Prediction of Bacterial Attachment to Polymers (2013)
Journal Article
Epa, V., Hook, A. L., Chang, C., Yang, J., Langer, R., Anderson, D. G., …Winkler, D. A. (2014). Modelling and Prediction of Bacterial Attachment to Polymers. Advanced Functional Materials, 24(14), 2085-2093. https://doi.org/10.1002/adfm.201302877

Infection by pathogenic bacteria on implanted and indwelling medical devices during surgery causes large morbidity and mortality worldwide. Attempts to ameliorate this important medical issue have included development of antimicrobial surfaces on mat... Read More about Modelling and Prediction of Bacterial Attachment to Polymers.

Ambient DESI and LESA-MS analysis of proteins adsorbed to a biomaterial surface using in-situ surface tryptic digestion (2013)
Journal Article
Rao, W., Celiz, A. D., Scurr, D. J., Alexander, M. R., & Barrett, D. A. (2013). Ambient DESI and LESA-MS analysis of proteins adsorbed to a biomaterial surface using in-situ surface tryptic digestion. Journal of The American Society for Mass Spectrometry, 24(12), https://doi.org/10.1007/s13361-013-0737-3

The detection and identification of proteins adsorbed onto biomaterial surfaces under ambient conditions has significant experimental advantages but has proven to be difficult to achieve with conventional measuring technologies. In this study, we pre... Read More about Ambient DESI and LESA-MS analysis of proteins adsorbed to a biomaterial surface using in-situ surface tryptic digestion.

Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes (2013)
Journal Article
Lui, L. T., Xue, X., Sui, C., Brown, A., Pritchard, D. I., Halliday, N., …Alexander, C. (2013). Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes. Nature Chemistry, 5(12), https://doi.org/10.1038/nchem.1793

Bacteria deploy a range of chemistries to regulate their behaviour and respond to their environment. Quorum sensing is one mean by which bacteria use chemical reactions to modulate pre-infection behaviour such as surface attachment. Polymers that can... Read More about Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes.

Development of a porous poly(DL-lactic acid-co-glycolic acid)-based scaffold for mastoid air-cell regeneration (2013)
Journal Article
Gould, T. W., Birchall, J. P., Mallick, A. S., Alliston, T., Lustig, L. R., Shakesheff, K. M., & Rahman, C. V. (2013). Development of a porous poly(DL-lactic acid-co-glycolic acid)-based scaffold for mastoid air-cell regeneration. Laryngoscope, 123(12), https://doi.org/10.1002/lary.24173

Objectives/Hypothesis: To develop a porous, biodegradable scaffold for mastoid air-cell regeneration. Study Design: In vitro development of a temperature-sensitive poly(DL-lactic acid-co-glycolic acid)/poly(ethylene glycol) (PLGA/PEG) scaffold tailo... Read More about Development of a porous poly(DL-lactic acid-co-glycolic acid)-based scaffold for mastoid air-cell regeneration.

Uptake and transport of B12-conjugated nanoparticles in airway epithelium (2013)
Journal Article
Fowler, R., Vllasaliu, D., Falcone, F. H., Garnett, M., Smith, B., Horsley, H., …Stolnik, S. (2013). Uptake and transport of B12-conjugated nanoparticles in airway epithelium. Journal of Controlled Release, 172(1), https://doi.org/10.1016/j.jconrel.2013.08.028

Non-invasive delivery of biotherapeutics, as an attractive alternative to injections, could potentially be achieved through the mucosal surfaces, utilizing nanoscale therapeutic carriers. However, nanoparticles do not readily cross the mucosal barrie... Read More about Uptake and transport of B12-conjugated nanoparticles in airway epithelium.

Desktop 3D printing of controlled release pharmaceutical bilayer tablets (2013)
Journal Article
Khaled, S. A., Burley, J. C., Alexander, M. R., & Roberts, C. J. (2014). Desktop 3D printing of controlled release pharmaceutical bilayer tablets. International Journal of Pharmaceutics, 461(1-2), 105-111. https://doi.org/10.1016/j.ijpharm.2013.11.021

Three dimensional (3D) printing was used as a novel medicine formulation technique for production of viable tablets capable of satisfying regulatory tests and matching the release of standard commercial tablets. Hydroxypropyl methylcellulose (HPMC 22... Read More about Desktop 3D printing of controlled release pharmaceutical bilayer tablets.

Self-reporting Scaffolds for 3-Dimensional Cell Culture (2013)
Journal Article
Harrington, H., Rose, F. R., Aylott, J. W., & Ghaemmaghami, A. M. (2013). Self-reporting Scaffolds for 3-Dimensional Cell Culture. Journal of Visualized Experiments, https://doi.org/10.3791/50608

Culturing cells in 3D on appropriate scaffolds is thought to better mimic the in vivo microenvironment and increase cell-cell interactions. The resulting 3D cellular construct can often be more relevant to studying the molecular events and cell-cell... Read More about Self-reporting Scaffolds for 3-Dimensional Cell Culture.

Comparison of osteogenic differentiation of embryonic stem cells and primary osteoblasts revealed by responses to IL-1?, TNF-?, and IFN-? (2013)
Journal Article
Sidney, L. E., Kirkham, G. R., & Buttery, L. D. (2013). Comparison of osteogenic differentiation of embryonic stem cells and primary osteoblasts revealed by responses to IL-1?, TNF-?, and IFN-?. Stem Cells and Development, 23(6), https://doi.org/10.1089/scd.2013.0336

There are well-established approaches for osteogenic differentiation of embryonic stem cells (ESCs), but few show direct comparison with primary osteoblasts or demonstrate differences in response to external factors. Here, we show comparative analysi... Read More about Comparison of osteogenic differentiation of embryonic stem cells and primary osteoblasts revealed by responses to IL-1?, TNF-?, and IFN-?.

Dissolution methodology for taste masked oral dosage forms (2013)
Journal Article
Gittings, S., Turnbull, N., Roberts, C., & Gershkovich, P. (2014). Dissolution methodology for taste masked oral dosage forms. Journal of Controlled Release, 173, 32-42. doi:10.1016/j.jconrel.2013.10.030

Conventional adult dosage forms are often not suitable for the paediatric and geriatric populations due to either swallowing difficulties or patient repulsion and a requirement for tailored dosing to individual compliance or physiological needs. Alte... Read More about Dissolution methodology for taste masked oral dosage forms.

A fluorescence-based assay suitable for quantitative analysis of deadenylase enzyme activity (2013)
Journal Article
Maryati, M., Kaur, I., Jadhav, G. P., Olotu-Umoren, L., Oveh, B., Hashmi, L., …Winkler, G. S. (2014). A fluorescence-based assay suitable for quantitative analysis of deadenylase enzyme activity. Nucleic Acids Research, 42(5), https://doi.org/10.1093/nar/gkt972

In eukaryotic cells, the shortening and removal of the poly(A) tail of cytoplasmic mRNA by deadenylase enzymes is a critical step in post-transcriptional gene regulation. The ribonuclease activity of deadenylase enzymes is attributed to either a DEDD... Read More about A fluorescence-based assay suitable for quantitative analysis of deadenylase enzyme activity.

Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain (2013)
Journal Article
Kelly, S., Chapman, R., Woodhams, S., Sagar, D. R., Turner, J., Burston, J., …Chapman, V. (2014). Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain. Annals of the Rheumatic Diseases, 74, https://doi.org/10.1136/annrheumdis-2013-203413

Objectives Blockade of transient receptor potential vanilloid 1 (TRPV1) with systemic antagonists attenuates osteoarthritis (OA) pain behaviour in rat models, but on-target-mediated hyperthermia has halted clinical trials. The present study investiga... Read More about Increased function of pronociceptive TRPV1 at the level of the joint in a rat model of osteoarthritis pain.

The P body protein LSm1 contributes to stimulation of hepatitis C virus translation, but not replication, by microRNA-122 (2013)
Journal Article
Roberts, A. P., Doidge, R., Tarr, A. W., & Jopling, C. L. (2014). The P body protein LSm1 contributes to stimulation of hepatitis C virus translation, but not replication, by microRNA-122. Nucleic Acids Research, 42(2), 1257-1269. https://doi.org/10.1093/nar/gkt941

The P body protein LSm1 stimulates translation and replication of hepatitis C virus (HCV). As the liver-specific microRNA-122 (miR-122) is required for HCV replication and is associated with P bodies, we investigated whether regulation of HCV by LSm1... Read More about The P body protein LSm1 contributes to stimulation of hepatitis C virus translation, but not replication, by microRNA-122.

Adjuvant chemotherapy for brain tumors delivered via a novel intra-cavity moldable polymer matrix (2013)
Journal Article
Rahman, C. V., Smith, S. J., Morgan, P. S., Langmack, K. A., Clarke, P. A., Ritchie, A. A., …Rahman, R. (2013). Adjuvant chemotherapy for brain tumors delivered via a novel intra-cavity moldable polymer matrix. PLoS ONE, 8(10), Article e77435. https://doi.org/10.1371/journal.pone.0077435

INTRODUCTION Polymer-based delivery systems offer innovative intra-cavity administration of drugs, with the potential to better target micro-deposits of cancer cells in brain parenchyma beyond the resected cavity. Here we evaluate clinical utility... Read More about Adjuvant chemotherapy for brain tumors delivered via a novel intra-cavity moldable polymer matrix.

Nanoparticle transport in epithelial cells: pathway switching through bioconjugation (2013)
Journal Article
Fowler, R., Vllasaliu, D., Trillo, F. F., Garnett, M., Alexander, C., Horsley, H., …Stolnik, S. (in press). Nanoparticle transport in epithelial cells: pathway switching through bioconjugation. Small, 9(19), https://doi.org/10.1002/smll.201202623

The understanding and control of nanoparticle transport into and through cellular compartments is central to biomedical applications of nanotechnology. Here, it is shown that the transport pathway of 50 nm polystyrene nanoparticles decorated with vit... Read More about Nanoparticle transport in epithelial cells: pathway switching through bioconjugation.

Interconnectivity and permeability of supercritical fluid-foamed scaffolds and the effect of their structural properties on cell distribution (2013)
Journal Article
Reinwald, Y., Johal, R. K., Ghaemmaghami, A. M., Rose, F. R., Howdle, S. M., & Shakesheff, K. M. (2014). Interconnectivity and permeability of supercritical fluid-foamed scaffolds and the effect of their structural properties on cell distribution. Polymer, 55(1), 435-444. https://doi.org/10.1016/j.polymer.2013.09.041

This study aims to investigate interconnectivity and permeability of scCO2-foamed scaffolds and the influence of structural scaffold properties on cell distribution. Supercritical fluid technology was utilized to fabricated scaffolds from 37 kDa, 53... Read More about Interconnectivity and permeability of supercritical fluid-foamed scaffolds and the effect of their structural properties on cell distribution.

Conversion of a non-selective adenosine receptor antagonist into A 3-selective high affinity fluorescent probes using peptide-based linkers (2013)
Journal Article
Vernall, A. J., Stoddart, L. A., Briddon, S. J., Ng, H. W., Laughton, C. A., Doughty, S. W., …Kellam, B. (2013). Conversion of a non-selective adenosine receptor antagonist into A 3-selective high affinity fluorescent probes using peptide-based linkers. Organic and Biomolecular Chemistry, 11(34), 5673-5682. https://doi.org/10.1039/c3ob41221k

Advances in fluorescence-based imaging technologies have helped propel the study of real-time biological readouts and analysis across many different areas. In particular the use of fluorescent ligands as chemical tools to study proteins such as G pro... Read More about Conversion of a non-selective adenosine receptor antagonist into A 3-selective high affinity fluorescent probes using peptide-based linkers.

Optimisation and Use of Humanised RBL NF-AT-GFP and NF-AT-DsRed Reporter Cell Lines Suitable for High-Throughput Scale Detection of Allergic Sensitisation in Array Format and Identification of the ECM–Integrin Interaction as Critical Factor (2013)
Journal Article
Wang, X., Cato, P., Lin, H., Li, T., Wan, D., Alcocer, M. J., & Falcone, F. H. (2014). Optimisation and Use of Humanised RBL NF-AT-GFP and NF-AT-DsRed Reporter Cell Lines Suitable for High-Throughput Scale Detection of Allergic Sensitisation in Array Format and Identification of the ECM–Integrin Interaction as Critical Factor. Molecular Biotechnology, 56(2), https://doi.org/10.1007/s12033-013-9689-x

We have previously described a microarray platform combining live basophils with protein arrays suitable for high-throughput detection of sensitisation against allergens. During optimisation of this technique, we observed severe losses of adhering ce... Read More about Optimisation and Use of Humanised RBL NF-AT-GFP and NF-AT-DsRed Reporter Cell Lines Suitable for High-Throughput Scale Detection of Allergic Sensitisation in Array Format and Identification of the ECM–Integrin Interaction as Critical Factor.