All Outputs (9)

TEM tomography of pores with application to computational nanoscale flows in nanoporous silicon nitride (NPN) (2018)
Journal Article
Madejski, G., Lucas, K., Pascut, F. C., Webb, K. F., & McGrath, J. L. (2018). TEM tomography of pores with application to computational nanoscale flows in nanoporous silicon nitride (NPN). Membranes, 8(2), Article 26. https://doi.org/10.3390/membranes8020026

Silicon nanomembrane technologies (NPN, pnc-Si, and others) have been used commercially as electron microscopy (EM) substrates, and as filters with nanometer-resolution size cut-offs. Combined with EM, these materials provide a platform for catching... Read More about TEM tomography of pores with application to computational nanoscale flows in nanoporous silicon nitride (NPN).

Rapid one-step biotinylation of biological and non-biological surfaces (2018)
Journal Article
Henry, S., Williams, E., Barr, K., Korchagina, E., Tuzikov, A., Ilyushina, N., …Bovin, N. (2018). Rapid one-step biotinylation of biological and non-biological surfaces. Scientific Reports, 8(1), Article 2845. https://doi.org/10.1038/s41598-018-21186-3

We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minu... Read More about Rapid one-step biotinylation of biological and non-biological surfaces.

Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells (2018)
Journal Article
Petrova, R. S., Webb, K. F., Vaghefi, E., Walker, K., Schey, K. L., & Donaldson, P. J. (2018). Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells. American Journal of Physiology - Cell Physiology, 314(2), C191-C201. https://doi.org/10.1152/ajpcell.00214.2017

Although the functionality of the lens water channels aquaporin 1 (AQP1; epithelium) and AQP0 (fiber cells) is well established, less is known about the role of AQP5 in the lens. Since in other tissues AQP5 functions as a regulated water channel with... Read More about Dynamic functional contribution of the water channel AQP5 to the water permeability of peripheral lens fiber cells.

Cell imaging by phonon microscopy: sub-optical wavelength ultrasound for non-invasive imaging (2017)
Journal Article
Perez-Cota, F., Smith, R. J., Moradi, E., Leija, A. L., Velickovic, K., Marques, L., …Clark, M. (2017). Cell imaging by phonon microscopy: sub-optical wavelength ultrasound for non-invasive imaging

The mechanical properties of cells play an important role in cell function and behavior. This paper presents recent developments that have enabled the use of laser-generated phonons (ultrasound) with sub-optical wavelengths to look inside living cell... Read More about Cell imaging by phonon microscopy: sub-optical wavelength ultrasound for non-invasive imaging.

High resolution 3D imaging of living cells with sub-optical wavelength phonons (2016)
Journal Article
Perez-Cota, F., Smith, R. J., Moradi, E., Marques, L., Webb, K. F., & Clark, M. (2016). High resolution 3D imaging of living cells with sub-optical wavelength phonons. Scientific Reports, 6, Article 39326. https://doi.org/10.1038/srep39326

Label-free imaging of living cells below the optical diffraction limit poses great challenges for optical microscopy. Biologically relevant structural information remains below the Rayleigh limit and beyond the reach of conventional microscopes. Supe... Read More about High resolution 3D imaging of living cells with sub-optical wavelength phonons.

Thin-film transducers for the detection and imaging of Brillouin oscillations in transmission on cultured cells (2015)
Journal Article
Perez-Cota, F., Smith, R. J., Moradi, E., Webb, K. F., & Clark, M. (in press). Thin-film transducers for the detection and imaging of Brillouin oscillations in transmission on cultured cells. Journal of Physics: Conference Series, 684(1), https://doi.org/10.1088/1742-6596/684/1/012003

Mechanical imaging and characterisation of biological cells has been a subject of interest for the last twenty years. Ultrasonic imaging based on the scanning acoustic microscope (SAM) and mechanical probing have been extensively reported. Large acou... Read More about Thin-film transducers for the detection and imaging of Brillouin oscillations in transmission on cultured cells.

Thin-film optoacoustic transducers for the subcellular Brillouin oscillation imaging of individual biological cells (2015)
Journal Article
Perez-Cota, F., Smith, R. J., Moradi, E., Marques, L., Webb, K. F., & Clark, M. (2015). Thin-film optoacoustic transducers for the subcellular Brillouin oscillation imaging of individual biological cells. Applied Optics, 54(28), https://doi.org/10.1364/AO.54.008388

Mechanical characterisation and imaging of biological tissue has piqued interest in the applicability to cell and tissue biology. One method, based on detection of Brillouin oscillations, has already lead to demonstrations on biological cells using u... Read More about Thin-film optoacoustic transducers for the subcellular Brillouin oscillation imaging of individual biological cells.

Optically excited nanoscale ultrasonic transducers (2015)
Journal Article
Smith, R. J., Cota, F. P., Marques, L., Chen, X., Arca, A., Webb, K., …Clark, M. (2015). Optically excited nanoscale ultrasonic transducers. Journal of the Acoustical Society of America, 137(1), 219-227. https://doi.org/10.1121/1.4904487

© 2015 Acoustical Society of America. In order to work at higher ultrasonic frequencies, for instance, to increase the resolution, it is necessary to fabricate smaller and higher frequency transducers. This paper presents an ultrasonic transducer cap... Read More about Optically excited nanoscale ultrasonic transducers.

Condenser-free contrast methods for transmitted-light microscopy (2014)
Journal Article
Webb, K. F. (2014). Condenser-free contrast methods for transmitted-light microscopy. Journal of Microscopy, 257(1), 8-22. https://doi.org/10.1111/jmi.12181

Phase contrast microscopy allows the study of highly transparent yet detail-rich specimens by producing intensity contrast from phase objects within the sample. Presented here is a generalized phase contrast illumination schema in which condenser opt... Read More about Condenser-free contrast methods for transmitted-light microscopy.