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Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes (2023)
Journal Article
Tutt, D. A. R., Guven-Ates, G., Kwong, W. Y., Simmons, R., Sang, F., Silvestri, G., …Sinclair, K. D. (2023). Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes. Frontiers in Endocrinology, 14, Article 1280847. https://doi.org/10.3389/fendo.2023.1280847

Background: In vitro maturation (IVM) of germinal vesicle intact oocytes prior to in vitro fertilization (IVF) is practiced widely in animals. In human assisted reproduction it is generally reserved for fertility preservation or where ovarian stimula... Read More about Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes.

Enhanced progesterone support during stimulated cycles of transvaginal follicular aspiration improves bovine in vitro embryo production (2023)
Journal Article
Simmons, R., Tutt, D. A., Tutt, D. A., Guven-Ates, G., Kwong, W. Y., Labrecque, R., …Sinclair, K. D. (2023). Enhanced progesterone support during stimulated cycles of transvaginal follicular aspiration improves bovine in vitro embryo production. Theriogenology, 199, 77-85. https://doi.org/10.1016/j.theriogenology.2023.01.003

The in vitro production (IVP) of cattle embryos requires that germinal-vesicle stage oocytes undergo a period of maturation in vitro prior to fertilization and culture to the blastocyst stage. Success of IVP in taurine cattle is enhanced following ov... Read More about Enhanced progesterone support during stimulated cycles of transvaginal follicular aspiration improves bovine in vitro embryo production.

Interspecific Variation in One-Carbon Metabolism within the Ovarian Follicle, Oocyte, and Preimplantation Embryo: Consequences for Epigenetic Programming of DNA Methylation (2021)
Journal Article
Clare, C. E., Pestinger, V., Kwong, W. Y., Tutt, D. A. R., Xu, J., Byrne, H. M., …Sinclair, K. D. (2021). Interspecific Variation in One-Carbon Metabolism within the Ovarian Follicle, Oocyte, and Preimplantation Embryo: Consequences for Epigenetic Programming of DNA Methylation. International Journal of Molecular Sciences, 22(4), Article 1838. https://doi.org/10.3390/ijms22041838

One-carbon (1C) metabolism provides methyl groups for the synthesis and/or methylation 20 of purines and pyrimidines, biogenic amines, proteins and phospholipids. Our understanding of 21 how 1C pathways operate, however, pertains mostly to the (rat)... Read More about Interspecific Variation in One-Carbon Metabolism within the Ovarian Follicle, Oocyte, and Preimplantation Embryo: Consequences for Epigenetic Programming of DNA Methylation.

Analysis of bovine blastocysts indicates ovarian stimulation does not induce chromosome errors, nor discordance between inner-cell mass and trophectoderm lineages (2020)
Journal Article
Tutt, D. A., Silvestri, G., Serrano-Albal, M., Simmons, R. J., Kwong, W. Y., Guven-Ates, G., …Sinclair, K. D. (2021). Analysis of bovine blastocysts indicates ovarian stimulation does not induce chromosome errors, nor discordance between inner-cell mass and trophectoderm lineages. Theriogenology, 161, 108-119. https://doi.org/10.1016/j.theriogenology.2020.11.021

© 2020 The Authors Contemporary systems for oocyte retrieval and culture of both cattle and human embryos are suboptimal with respect to pregnancy outcomes following transfer. In humans, chromosome abnormalities are the leading cause of early pregnan... Read More about Analysis of bovine blastocysts indicates ovarian stimulation does not induce chromosome errors, nor discordance between inner-cell mass and trophectoderm lineages.

Added dietary cobalt or vitamin B12, or injecting vitamin B12 does not improve performance or indicators of ketosis in pre- and post-partum Holstein-Friesian dairy cows (2018)
Journal Article
Weerathilake, W. A. D. V., Brassington, A. H., Williams, S. J., Kwong, W. Y., Sinclair, L. A., & Sinclair, K. D. (2019). Added dietary cobalt or vitamin B12, or injecting vitamin B12 does not improve performance or indicators of ketosis in pre- and post-partum Holstein-Friesian dairy cows. animal, 13(4), 750-759. https://doi.org/10.1017/S175173111800232X

Vitamin B12 is synthesised in the rumen from cobalt (Co) and has a major role in metabolism in the peri-paturient period, although few studies have evaluated the effect of the dietary inclusion of Co, vitamin B12 or injecting vitamin B12 on the metab... Read More about Added dietary cobalt or vitamin B12, or injecting vitamin B12 does not improve performance or indicators of ketosis in pre- and post-partum Holstein-Friesian dairy cows.

Physiological responses of cultured bovine granulosa cells to elevated temperatures under low and high oxygen in the presence of different concentrations of melatonin (2017)
Journal Article
Zeebaree, B. K., Kwong, W. Y., Mann, G. E., Gutierrez, C. G., & Sinclair, K. D. (2018). Physiological responses of cultured bovine granulosa cells to elevated temperatures under low and high oxygen in the presence of different concentrations of melatonin. Theriogenology, 105, https://doi.org/10.1016/j.theriogenology.2017.09.014

Our understanding of the effects of temperature on granulosa cell (GC) physiology is primarily limited to in vitro studies conducted under atmospheric (approx 20% O2) conditions. In the current series of factorial experiments we identify important ef... Read More about Physiological responses of cultured bovine granulosa cells to elevated temperatures under low and high oxygen in the presence of different concentrations of melatonin.