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Comparison and optimization of ten phage encoded serine integrases for genome engineering in Saccharomyces cerevisiae (2016)
Journal Article
Xu, Z., & Brown, W. (2016). Comparison and optimization of ten phage encoded serine integrases for genome engineering in Saccharomyces cerevisiae. BMC Biotechnology, 16, doi:10.1186/s12896-016-0241-5

Background: Phage-encoded serine integrases, such as ϕC31 integrase, are widely used for genome engineering but have not been optimized for use in Saccharomyces cerevisiae although this organism is a widely used organism in biotechnology. Results:... Read More

Kinetochore assembly and heterochromatin formation occur autonomously in Schizosaccharomyces pombe (2014)
Journal Article
Brown, W. R., Thomas, G., Lee, N. C., Blythe, M., Liti, G., Warringer, J., & Loose, M. W. (2014). Kinetochore assembly and heterochromatin formation occur autonomously in Schizosaccharomyces pombe. Proceedings of the National Academy of Sciences, 111(5), doi:10.1073/pnas.1216934111. ISSN 0027-8424

Kinetochores in multicellular eukaryotes are usually associated with heterochromatin. Whether this heterochromatin simply promotes the cohesion necessary for accurate chromosome segregation at cell division or whether it also has a role in kinetochor... Read More

Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome (2013)
Journal Article
for the integration of DNA into the human genome. BMC Biotechnology, 13, doi:10.1186/1472-6750-13-87. ISSN 1472-6750

Background: Phage-encoded serine integrases, such as φC31 integrase, are widely used for genome engineering. Fifteen such integrases have been described but their utility for genome engineering has not been compared in uniform assays. Results: We h... Read More