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Engineering nanowires in bacteria to elucidate electron transport structural–functional relationships (2023)
Journal Article
Myers, B., Catrambone, F., Allen, S., Hill, P. J., Kovacs, K., & Rawson, F. J. (2023). Engineering nanowires in bacteria to elucidate electron transport structural–functional relationships. Scientific Reports, 13(1), Article 8843. https://doi.org/10.1038/s41598-023-35553-2

Bacterial pilin nanowires are protein complexes, suggested to possess electroactive capabilities forming part of the cells’ bioenergetic programming. Their role is thought to be linked to facilitating electron transfer between cells and the external... Read More about Engineering nanowires in bacteria to elucidate electron transport structural–functional relationships.

Development of p-Tau Differentiated Cell Model of Alzheimer’s Disease to Screen Novel Acetylcholinesterase Inhibitors (2022)
Journal Article
Uras, G., Li, X., Manca, A., Pantaleo, A., Bo, M., Xu, J., …Zhu, Z. (2022). Development of p-Tau Differentiated Cell Model of Alzheimer’s Disease to Screen Novel Acetylcholinesterase Inhibitors. International Journal of Molecular Sciences, 23(23), Article 14794. https://doi.org/10.3390/ijms232314794

Alzheimer’s disease (AD) is characterized by an initial accumulation of amyloid plaques and neurofibrillary tangles, along with the depletion of cholinergic markers. The currently available therapies for AD do not present any disease-modifying effect... Read More about Development of p-Tau Differentiated Cell Model of Alzheimer’s Disease to Screen Novel Acetylcholinesterase Inhibitors.

Design, Synthesis, and Biological Evaluation of Novel Chromanone Derivatives as Multifunctional Agents for the Treatment of Alzheimer's Disease (2022)
Journal Article
Li, X., Li, T., Zhan, F., Cheng, F., Lu, L., Zhang, B., …Yao, H. (2022). Design, Synthesis, and Biological Evaluation of Novel Chromanone Derivatives as Multifunctional Agents for the Treatment of Alzheimer's Disease. ACS Chemical Neuroscience, 13(23), 3488-3501. https://doi.org/10.1021/acschemneuro.2c00520

Based on a multitarget strategy, a series of novel chromanone-1-benzyl-1,2,3,6-tetrahydropyridin hybrids were identified for the potential treatment of Alzheimer's disease (AD). Biological evaluation demonstrated that these hybrids exhibited signific... Read More about Design, Synthesis, and Biological Evaluation of Novel Chromanone Derivatives as Multifunctional Agents for the Treatment of Alzheimer's Disease.

Discovery of novel hybrids containing clioquinol−1-benzyl-1,2,3,6-tetrahydropyridine as multi-target-directed ligands (MTDLs) against Alzheimer's disease (2022)
Journal Article
Li, X., Li, T., Zhang, P., Li, X., Lu, L., Sun, Y., …Xu, J. (2022). Discovery of novel hybrids containing clioquinol−1-benzyl-1,2,3,6-tetrahydropyridine as multi-target-directed ligands (MTDLs) against Alzheimer's disease. European Journal of Medicinal Chemistry, 244, Article 114841. https://doi.org/10.1016/j.ejmech.2022.114841

Based on the multitarget strategy, a series of novel clioquinol−1-benzyl-1,2,3,6-tetrahydropyridine hybrids were identified for the potential treatment of Alzheimer's disease (AD). Biological evaluation in vitro revealed that these hybrids exhibited... Read More about Discovery of novel hybrids containing clioquinol−1-benzyl-1,2,3,6-tetrahydropyridine as multi-target-directed ligands (MTDLs) against Alzheimer's disease.

Novel inhibitors of AChE and Aβ aggregation with neuroprotective properties as lead compounds for the treatment of Alzheimer's disease (2022)
Journal Article
Liu, Y., Uras, G., Onuwaje, I., Li, W., Yao, H., Xu, S., …Xu, J. (2022). Novel inhibitors of AChE and Aβ aggregation with neuroprotective properties as lead compounds for the treatment of Alzheimer's disease. European Journal of Medicinal Chemistry, 235, Article 114305. https://doi.org/10.1016/j.ejmech.2022.114305

A series of sulfone analogs of donepezil were designed and synthesized as novel acetylcholinesterase (AChE) inhibitors with the potent inhibiting Aβ aggregation and providing neuroprotective effects as potential modalities for Alzheimer's disease (AD... Read More about Novel inhibitors of AChE and Aβ aggregation with neuroprotective properties as lead compounds for the treatment of Alzheimer's disease.

In vivo Evaluation of a Newly Synthesized Acetylcholinesterase Inhibitor in a Transgenic Drosophila Model of Alzheimer’s Disease (2021)
Journal Article
Uras, G., Manca, A., Zhang, P., Markus, Z., Mack, N., Allen, S., …Zhu, Z. (2021). In vivo Evaluation of a Newly Synthesized Acetylcholinesterase Inhibitor in a Transgenic Drosophila Model of Alzheimer’s Disease. Frontiers in Neuroscience, 15, Article 691222. https://doi.org/10.3389/fnins.2021.691222

Alzheimer’s disease is a neurodegenerative disease characterized by disrupted memory, learning functions, reduced life expectancy, and locomotor dysfunction, as a result of the accumulation and aggregation of amyloid peptides that cause neuronal dama... Read More about In vivo Evaluation of a Newly Synthesized Acetylcholinesterase Inhibitor in a Transgenic Drosophila Model of Alzheimer’s Disease.

Discovery of Novel Tacrine–Pyrimidone Hybrids as Potent Dual AChE/GSK-3 Inhibitors for the Treatment of Alzheimer’s Disease (2021)
Journal Article
Yao, H., Uras, G., Zhang, P., Xu, S., Yin, Y., Liu, J., …Xu, J. (2021). Discovery of Novel Tacrine–Pyrimidone Hybrids as Potent Dual AChE/GSK-3 Inhibitors for the Treatment of Alzheimer’s Disease. Journal of Medicinal Chemistry, 64(11), 7483-7506. https://doi.org/10.1021/acs.jmedchem.1c00160

Based on a multitarget strategy, a series of novel tacrine–pyrimidone hybrids were identified for the potential treatment of Alzheimer’s disease (AD). Biological evaluation results demonstrated that these hybrids exhibited significant inhibitory acti... Read More about Discovery of Novel Tacrine–Pyrimidone Hybrids as Potent Dual AChE/GSK-3 Inhibitors for the Treatment of Alzheimer’s Disease.

Localized induction of gene expression in embryonic stem cell aggregates using holographic optical tweezers to create biochemical gradients (2019)
Journal Article
Kirkham, G. R., Ware, J., Upton, T., Allen, S., Shakesheff, K. M., & Buttery, L. D. (2020). Localized induction of gene expression in embryonic stem cell aggregates using holographic optical tweezers to create biochemical gradients. Regenerative Engineering and Translational Medicine, 6(3), 251–261. https://doi.org/10.1007/s40883-019-00114-5

Three dimensional (3D) cell models that mimic the structure and function of native tissues are enabling more detailed study of physiological and pathological mechanisms in vitro. We have previously demonstrated the ability to build and manipulate 3D... Read More about Localized induction of gene expression in embryonic stem cell aggregates using holographic optical tweezers to create biochemical gradients.

Synthetic glycopolymers as modulators of protein aggregation: influence of chemical composition, topology and concentration (2018)
Journal Article
Madeira do Ó, J., Mastrotto, F., Francini, N., Allen, S., van der Walle, C., Stolnik, S., & Mantovani, G. (in press). Synthetic glycopolymers as modulators of protein aggregation: influence of chemical composition, topology and concentration. Journal of Materials Chemistry B, 7(6), https://doi.org/10.1039/c7tb02720f

Novel drug excipients are required to achieve stable formulations of protein drug candidates. Synthetic glycopolymers have been shown in some cases to improve protein formulation stability, although their structure function relationship remains unkno... Read More about Synthetic glycopolymers as modulators of protein aggregation: influence of chemical composition, topology and concentration.

Engineered Polymer–Transferrin Conjugates as Self-Assembling Targeted Drug Delivery Systems (2017)
Journal Article
Makwana, H., Mastrotto, F., Magnusson, J. P., Sleep, D., Hay, J., Nicholls, K. J., …Alexander, C. (2017). Engineered Polymer–Transferrin Conjugates as Self-Assembling Targeted Drug Delivery Systems. Biomacromolecules, 18(5), 1532-1543. https://doi.org/10.1021/acs.biomac.7b00101

Polymer–protein conjugates can be engineered to self-assemble into discrete and well-defined drug delivery systems, which combine the advantages of receptor targeting and controlled drug release. We designed specific conjugates of the iron-binding an... Read More about Engineered Polymer–Transferrin Conjugates as Self-Assembling Targeted Drug Delivery Systems.

The effect of protein concentration on the viscosity of a recombinant albumin solution formulation (2016)
Journal Article
Gonçalves, A. D., Alexander, C., Roberts, C. J., Spain, S. G., Uddin, S., & Allen, S. (2016). The effect of protein concentration on the viscosity of a recombinant albumin solution formulation. RSC Advances, 6(18), 15143-15154. https://doi.org/10.1039/c5ra21068b

The effect of protein concentration on solution viscosity in a commercially available biopharmaceutical formulation of recombinant albumin (rAlbumin) was studied. The level of protein aggregation with concentration and its impact on solution viscosit... Read More about The effect of protein concentration on the viscosity of a recombinant albumin solution formulation.

Phosphonium polymethacrylates for siRNA delivery: effect of polymer and RNA structural parameters on polyplex assembly and gene knockdown (2015)
Journal Article
Loczenski Rose, V., Shubber, S., Sajeesh, S., Spain, S. G., Puri, S., Allen, S., …Mantovani, G. (2015). Phosphonium polymethacrylates for siRNA delivery: effect of polymer and RNA structural parameters on polyplex assembly and gene knockdown. Biomacromolecules, 16(11), https://doi.org/10.1021/acs.biomac.5b00898

Synthetic polymers containing quaternary phosphonium salts are an emerging class of materials for the delivery of oligo/polynucleotides. In this work, cationic phosphonium salt-containing polymethacrylates –and their corresponding ammonium analogues–... Read More about Phosphonium polymethacrylates for siRNA delivery: effect of polymer and RNA structural parameters on polyplex assembly and gene knockdown.

Precision assembly of complex cellular microenvironments using holographic optical tweezers (2015)
Journal Article
Kirkham, G. R., Britchford, E., Upton, T., Ware, J., Gibson, G., Devaud, Y., …Shakesheff, K. M. (2015). Precision assembly of complex cellular microenvironments using holographic optical tweezers. Scientific Reports, 5, Article 8577. https://doi.org/10.1038/srep08577

The accurate study of cellular microenvironments is limited by the lack of technologies that can manipulate cells in 3D at a sufficiently small length scale. The ability to build and manipulate multicellular microscopic structures will facilitate a m... Read More about Precision assembly of complex cellular microenvironments using holographic optical tweezers.

Analysis of leaf surfaces using scanning ion conductance microscopy (2015)
Journal Article
Walker, S. C., Allen, S., Bell, G., & Roberts, C. J. (2015). Analysis of leaf surfaces using scanning ion conductance microscopy. Journal of Microscopy, 258(2), https://doi.org/10.1111/jmi.12225

Leaf surfaces are highly complex functional systems with well defined chemistry and structure dictating the barrier and transport properties of the leaf cuticle. It is a significant imaging challenge to analyse the very thin and often complex wax-lik... Read More about Analysis of leaf surfaces using scanning ion conductance microscopy.

Study of NAP adsorption and assembly on the surface of HOPG (2014)
Journal Article
Korolkov, V. V., Allen, S., Roberts, C. J., Gozes, I., & Tendler, S. J. (2014). Study of NAP adsorption and assembly on the surface of HOPG. Peptides, 62, 55-58. https://doi.org/10.1016/j.peptides.2014.09.017

NAP is an octapeptide that has demonstrated a neuroprotective/therapeutic efficacy at very low concentrations in preclinical studies and in a number of clinical trials. Yet little is known about its structural organization at low concentrations. Here... Read More about Study of NAP adsorption and assembly on the surface of HOPG.

Bimolecular porous supramolecular networks deposited from solution on layered materials: graphite, boron nitride and molybdenum disulphide (2014)
Journal Article
Korolkov, V. V., Svatek, S. A., Allen, S., Roberts, C. J., Tendler, S. J., Taniguchi, T., …Beton, P. H. (2014). Bimolecular porous supramolecular networks deposited from solution on layered materials: graphite, boron nitride and molybdenum disulphide. Chemical Communications, 50(64), https://doi.org/10.1039/C4CC03720K

A two-dimensional porous network formed from perylene tetracarboxylic diimide (PTCDI) and melamine may be deposited from solution on the surfaces of highly oriented pyrolytic graphite (HOPG), hexagonal boron nitride (hBN) and molybdenum disulphide (... Read More about Bimolecular porous supramolecular networks deposited from solution on layered materials: graphite, boron nitride and molybdenum disulphide.

Atomic force microscopy study of human amylin (20-29) fibrils (2005)
Journal Article
Sedman, V. L., Allen, S., Chan, W. C., Davies, M. C., Roberts, C. J., Tendler, S. J., & Williams, P. M. (2005). Atomic force microscopy study of human amylin (20-29) fibrils. Protein and Peptide Letters, 12(1), 79-83. https://doi.org/10.2174/0929866053406129

Here we present atomic force microscopy images of the fibrils formed by human amylin(20-29). This peptide is a fragment of the polypeptide amylin, the major proteinaceous component of amyloid deposits found in cases of type-II diabetes mellitus. Our... Read More about Atomic force microscopy study of human amylin (20-29) fibrils.