Research Repository

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Functional identification of the prnABCD operon and its regulation in Serratia plymuthica (2018)
Journal Article
Liu, X., Yu, X., Yang, Y., Heeb, S., Gao, S., Chan, K. G., …Gao, K. (in press). Functional identification of the prnABCD operon and its regulation in Serratia plymuthica. Applied Microbiology and Biotechnology, doi:10.1007/s00253-018-8857-0. ISSN 0175-7598

The antibiotic pyrrolnitrin (PRN) is a tryptophan-derived secondary metabolite that plays an important role in the biocontrol of plant diseases due to its broad-spectrum of antimicrobial activities. The PRN biosynthetic gene cluster remains to be cha... Read More

Optimised chronic infection models demonstrate that siderophore ‘cheating’ in Pseudomonas aeruginosa is context specific (2017)
Journal Article
Harrison, F., McNally, A., da Silva, A. C., Heeb, S., & Diggle, S. P. (in press). Optimised chronic infection models demonstrate that siderophore ‘cheating’ in Pseudomonas aeruginosa is context specific. ISME Journal, doi:10.1038/ismej.2017.103. ISSN 1751-7362

The potential for siderophore mutants of Pseudomonas aeruginosa to attenuate virulence during infection, and the possibility of exploiting this for clinical ends, have attracted much discussion. This has largely been based on the results of in vitro... Read More

Identification of a Pseudomonas aeruginosa PAO1 DNA methyltransferase, its Targets, and physiological roles (2017)
Journal Article
Doberenz, S., Eckweiler, D., Reichert, O., Jensen, V., Bunk, B., Spröer, C., …Häussler, S. (2017). Identification of a Pseudomonas aeruginosa PAO1 DNA methyltransferase, its Targets, and physiological roles. mBio, 8(1), doi:10.1128/mBio.02312-16. ISSN 2150-7511

DNA methylation is widespread among prokaryotes, and most DNA methylation reactions are catalyzed by adenine DNA methyltransferases, which are part of restriction-modification (R-M) systems. R-M systems are known for their role in the defense against... Read More

RsmW, Pseudomonas aeruginosa small non-coding RsmA-binding RNA upregulated in biofilm versus planktonic growth conditions (2016)
Journal Article
Miller, C. L., Romero, M., Karna, S. R., Chen, T., Heeb, S., & Leung, K. P. (2016). RsmW, Pseudomonas aeruginosa small non-coding RsmA-binding RNA upregulated in biofilm versus planktonic growth conditions. BMC Microbiology, 16, doi:10.1186/s12866-016-0771-y. ISSN 1471-2180

BACKGROUND: Biofilm development, specifically the fundamentally adaptive switch from acute to chronic infection phenotypes, requires global regulators and small non-coding regulatory RNAs (sRNAs). This work utilized RNA-sequencing (RNA-seq) to detect... Read More

Synthesis and cell-free cloning of DNA libraries using programmable microfluidics (2015)
Journal Article
Ben Yehezkel, T., Rival, A., Raz, O., Cohen, R., Marx, Z., Cámara, M., …Shapiro, E. (2016). Synthesis and cell-free cloning of DNA libraries using programmable microfluidics. Nucleic Acids Research, 44(4), doi:10.1093/nar/gkv1087. ISSN 0305-1048

Microfluidics may revolutionize our ability to write synthetic DNA by addressing several fundamental limitations associated with generating novel genetic constructs. Here we report the first de novo synthesis and cell-free cloning of custom DNA libra... Read More

Clinical utilization of genomics data produced by the international Pseudomonas aeruginosa consortium (2015)
Journal Article
Freschi, L., Jeukens, J., Kukavica-Ibrulj, I., Boyle, B., Dupont, M., Laroche, J., …Levesque, R. C. (in press). Clinical utilization of genomics data produced by the international Pseudomonas aeruginosa consortium. Frontiers in Microbiology, 6(1036), doi:10.3389/fmicb.2015.01036. ISSN 1664-302X

The International Pseudomonas aeruginosa Consortium is sequencing over 1000 genomes and building an analysis pipeline for the study of Pseudomonas genome evolution, antibiotic resistance and virulence genes. Metadata, including genomic and phenotypic... Read More

Biotic inactivation of the Pseudomonas aeruginosa quinolone signal molecule (2015)
Journal Article
Soh, E. Y., Chhabra, S. R., Halliday, N., Heeb, S., Müller, C., Birmes, F. S., …Williams, P. (2015). Biotic inactivation of the Pseudomonas aeruginosa quinolone signal molecule. Environmental Microbiology, 17(11), doi:10.1111/1462-2920.12857. ISSN 1462-2912

In Pseudomonas aeruginosa, quorum sensing (QS) regulates the production of secondary metabolites, many of which are antimicrobials that impact on polymicrobial community composition. Consequently, quenching QS modulates the environmental impact of P.... Read More