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All Outputs (13)

Development, characterization, and evaluation of a simple polymicrobial colony biofilm model for testing of antimicrobial wound dressings (2024)
Journal Article
Robertson, S. N., Romero, M., Fenn, S., Riedi, P. L. K., & Cámara, M. (2024). Development, characterization, and evaluation of a simple polymicrobial colony biofilm model for testing of antimicrobial wound dressings. Journal of Applied Microbiology, 135(3), Article lxae042. https://doi.org/10.1093/jambio/lxae042

Chronic wound infections are generally of polymicrobial nature with aerobic and anaerobic bacteria, as well as fungi frequently observed in them. Wound treatment involves a series of steps, including debridement of the wound, flushing, and often the... Read More about Development, characterization, and evaluation of a simple polymicrobial colony biofilm model for testing of antimicrobial wound dressings.

Ciprofloxacin poly(β-amino ester) conjugates enhance antibiofilm activity and slow the development of resistance (2024)
Journal Article
Kasza, K., Richards, B., Jones, S., Romero, M., Robertson, S. N., Hardie, K. R., …Alexander, C. (2024). Ciprofloxacin poly(β-amino ester) conjugates enhance antibiofilm activity and slow the development of resistance. ACS Applied Materials and Interfaces, 16(5), 5412-5425. https://doi.org/10.1021/acsami.3c14357

To tackle the emerging antibiotic resistance crisis, novel antimicrobial approaches are urgently needed. Bacterial biofilms are a particular concern in this context as they are responsible for over 80% of bacterial infections and are inherently more... Read More about Ciprofloxacin poly(β-amino ester) conjugates enhance antibiofilm activity and slow the development of resistance.

Design, Synthesis, and Evaluation of New 1H-Benzo[d]imidazole Based PqsR Inhibitors as Adjuvant Therapy for Pseudomonas aeruginosa Infections (2024)
Journal Article
Soukarieh, F., Mashabi, A., Richardson, W., Oton, E. V., Romero, M., Dubern, J., …Cámara, M. (2024). Design, Synthesis, and Evaluation of New 1H-Benzo[d]imidazole Based PqsR Inhibitors as Adjuvant Therapy for Pseudomonas aeruginosa Infections. Journal of Medicinal Chemistry, 67(2), 1008-1023. https://doi.org/10.1021/acs.jmedchem.3c00973

Pseudomonas aeruginosa is one of the top priority pathogens that requires immediate attention according to the World Health Organisation (WHO). Due to the alarming shortage of novel antimicrobials, targeting quorum sensing (QS), a bacterial cell to c... Read More about Design, Synthesis, and Evaluation of New 1H-Benzo[d]imidazole Based PqsR Inhibitors as Adjuvant Therapy for Pseudomonas aeruginosa Infections.

Probing Interkingdom Signaling Molecules via Liquid Extraction Surface Analysis-Mass Spectrometry (2023)
Journal Article
Robertson, S. N., Soukarieh, F., White, T. M., Camara, M., Romero, M., & Griffiths, R. L. (2023). Probing Interkingdom Signaling Molecules via Liquid Extraction Surface Analysis-Mass Spectrometry. Analytical Chemistry, 95(11), 5079-5086. https://doi.org/10.1021/acs.analchem.2c05703

Previously, metabolites diffused or secreted from microbial samples have been analyzed via liquid chromatography–mass spectrometry (LC–MS) approaches following lengthy extraction protocols. Here, we present a model system for growing biofilms on disc... Read More about Probing Interkingdom Signaling Molecules via Liquid Extraction Surface Analysis-Mass Spectrometry.

Translational challenges and opportunities in biofilm science: a BRIEF for the future (2022)
Journal Article
Highmore, C. J., Melaugh, G., Morris, R. J., Parker, J., Direito, S. O. L., Romero, M., …Bamford, N. C. (2022). Translational challenges and opportunities in biofilm science: a BRIEF for the future. npj Biofilms and Microbiomes, 8, Article 68. https://doi.org/10.1038/s41522-022-00327-7

Biofilms are increasingly recognised as a critical global issue in a multitude of industries impacting health, food and water security, marine sector, and industrial processes resulting in estimated economic cost of $5 trillion USD annually. A major... Read More about Translational challenges and opportunities in biofilm science: a BRIEF for the future.

ToxR is a c-di-GMP binding protein that modulates surface-associated behaviour in Pseudomonas aeruginosa (2022)
Journal Article
Camara, M., Dubern, J., Romero, M., Mai-Prochnow, A., Messina, M., Trampari, E., …Heeb, S. (2022). ToxR is a c-di-GMP binding protein that modulates surface-associated behaviour in Pseudomonas aeruginosa. npj Biofilms and Microbiomes, 8, Article 64. https://doi.org/10.1038/s41522-022-00325-9

Pseudomonas aeruginosa uses multiple protein regulators that work in tandem to control the production of a wide range of virulence factors and facilitate rapid adaptation to diverse environmental conditions. In this opportunistic pathogen, ToxR was k... Read More about ToxR is a c-di-GMP binding protein that modulates surface-associated behaviour in Pseudomonas aeruginosa.

Design and Evaluation of New Quinazolin-4(3 H)-one Derived PqsR Antagonists as Quorum Sensing Quenchers in Pseudomonas aeruginosa (2021)
Journal Article
Soukarieh, F., Mashabi, A., Richardson, W., Oton, E. V., Romero, M., Roberston, S. N., …Cámara, M. (2021). Design and Evaluation of New Quinazolin-4(3 H)-one Derived PqsR Antagonists as Quorum Sensing Quenchers in Pseudomonas aeruginosa. ACS Infectious Diseases, 7(9), 2666-2685. https://doi.org/10.1021/acsinfecdis.1c00175

P. aeruginosa (PA) continues to pose a threat to global public health due to its high levels of antimicrobial resistance (AMR). The ongoing AMR crisis has led to an alarming shortage of effective treatments for resistant microbes, and hence there is... Read More about Design and Evaluation of New Quinazolin-4(3 H)-one Derived PqsR Antagonists as Quorum Sensing Quenchers in Pseudomonas aeruginosa.

Hit Identification of New Potent PqsR Antagonists as Inhibitors of Quorum Sensing in Planktonic and Biofilm Grown Pseudomonas aeruginosa (2020)
Journal Article
Soukarieh, F., Liu, R., Romero, M., Roberston, S. N., Richardson, W., Lucanto, S., …Stocks, M. J. (2020). Hit Identification of New Potent PqsR Antagonists as Inhibitors of Quorum Sensing in Planktonic and Biofilm Grown Pseudomonas aeruginosa. Frontiers in Chemistry, 8, Article 204. https://doi.org/10.3389/fchem.2020.00204

© Copyright © 2020 Soukarieh, Liu, Romero, Roberston, Richardson, Lucanto, Oton, Qudus, Mashabi, Grossman, Ali, Sou, Kukavica-Ibrulj, Levesque, Bergström, Halliday, Mistry, Emsley, Heeb, Williams, Cámara and Stocks. Current treatments for Pseudomonas... Read More about Hit Identification of New Potent PqsR Antagonists as Inhibitors of Quorum Sensing in Planktonic and Biofilm Grown Pseudomonas aeruginosa.

Internal embryonic development in a non-copulatory, egg-laying teleost, the three-spined stickleback, Gasterosteus aculeatus (2019)
Journal Article
Dean, L. L., Robertson, S., Mahmud, M., & Maccoll, A. D. C. (2019). Internal embryonic development in a non-copulatory, egg-laying teleost, the three-spined stickleback, Gasterosteus aculeatus. Scientific Reports, 9(1), Article 2395. https://doi.org/10.1038/s41598-019-38584-w

The switch from egg-laying to retaining and giving birth to live young is a major transition in the history of life. Despite its repeated evolution across the fishes, records of intermediate phenotypes are vanishingly rare, with only two known cases... Read More about Internal embryonic development in a non-copulatory, egg-laying teleost, the three-spined stickleback, Gasterosteus aculeatus.

A genetics-based approach confirms immune associations with life history across multiple populations of an aquatic vertebrate (Gasterosteus aculeatus) (2018)
Journal Article
Whiting, J. R., Magalhaes, I. S., Singkam, A. R., Robertson, S., D'Agostino, D., Bradley, J. E., & MacColl, A. D. (2018). A genetics-based approach confirms immune associations with life history across multiple populations of an aquatic vertebrate (Gasterosteus aculeatus). Molecular Ecology, 27(15), 3174-3191. https://doi.org/10.1111/mec.14772

Understanding how wild immune variation covaries with other traits can reveal how costs and trade?offs shape immune evolution in the wild. Divergent life history strategies may increase or alleviate immune costs, helping shape immune variation in a c... Read More about A genetics-based approach confirms immune associations with life history across multiple populations of an aquatic vertebrate (Gasterosteus aculeatus).

Eda haplotypes in three-spined stickleback are associated with variation in immune gene expression (2017)
Journal Article
Robertson, S., Bradley, J. E., & MacColl, A. D. (in press). Eda haplotypes in three-spined stickleback are associated with variation in immune gene expression. Scientific Reports, 7, https://doi.org/10.1038/srep42677

Haplotypes underlying local adaptation and speciation are predicted to have numerous phenotypic effects, but few genes involved have been identified, with much work to date concentrating on visible, morphological, phenotypes. The link between genes c... Read More about Eda haplotypes in three-spined stickleback are associated with variation in immune gene expression.

No evidence of local adaptation of immune responses to Gyrodactylus in three-spined stickleback (Gasterosteus aculeatus) (2016)
Journal Article
Robertson, S., Bradley, J. E., & MacColl, A. D. (2017). No evidence of local adaptation of immune responses to Gyrodactylus in three-spined stickleback (Gasterosteus aculeatus). Fish and Shellfish Immunology, 60, 275-281. https://doi.org/10.1016/j.fsi.2016.11.058

© 2016 The Authors Parasitism represents one of the most widespread lifestyles in the animal kingdom, with the potential to drive coevolutionary dynamics with their host population. Where hosts and parasites evolve together, we may find local adaptat... Read More about No evidence of local adaptation of immune responses to Gyrodactylus in three-spined stickleback (Gasterosteus aculeatus).

Measuring the immune system of the three-spined stickleback: investigating natural variation by quantifying immune expression in the laboratory and the wild (2015)
Journal Article
Robertson, S., Bradley, J. E., & MacColl, A. D. (2016). Measuring the immune system of the three-spined stickleback: investigating natural variation by quantifying immune expression in the laboratory and the wild. Molecular Ecology Resources, 16(3), https://doi.org/10.1111/1755-0998.12497

Current understanding of the immune system comes primarily from lab-based studies. There has been substantial interest in examining how it functions in the wild, but studies have been limited by a lack of appropriate assays and study species. The thr... Read More about Measuring the immune system of the three-spined stickleback: investigating natural variation by quantifying immune expression in the laboratory and the wild.