Research Repository

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Macrophage-derived IL-1β and TNF-α regulate arginine metabolism in neuroblastoma (2018)
Journal Article
Fultang, L., Gamble, L. D., Gneo, L., Berry, A. M., Egan, S. A., De Bie, F., …Mussai, F. (2018). Macrophage-derived IL-1β and TNF-α regulate arginine metabolism in neuroblastoma. Cancer Research, doi:10.1158/0008-5472.can-18-2139

Neuroblastoma is the most common childhood solid tumor, yet the prognosis for high-risk disease remains poor. We demonstrate here that arginase 2 (ARG2) drives neuroblastoma cell proliferation via regulation of arginine metabolism. Targeting arginine... Read More

Metabolic therapy with PEG-arginase induces a sustained complete remission in immunotherapy-resistant melanoma (2018)
Journal Article
De Santo, C., Cheng, P., Beggs, A., Egan, S., Bessudo, A., & Mussai, F. (2018). Metabolic therapy with PEG-arginase induces a sustained complete remission in immunotherapy-resistant melanoma. Journal of Hematology and Oncology, 11, doi:10.1186/s13045-018-0612-6. ISSN 1756-8722

Background Metastatic melanoma is an aggressive skin cancer with a poor prognosis. Current treatment strategies for high-stage melanoma are based around the use of immunotherapy with immune checkpoint inhibitors such as anti-PDL1 or anti-CTLA4 ant... Read More

The bio-physics of condensation of divalent cations into the bacterial wall has implications for growth of Gram-positive bacteria (2016)
Journal Article
Rauch, C., Cherkaoui-Rbati, M., Egan, S. A., & Leigh, J. A. (2017). The bio-physics of condensation of divalent cations into the bacterial wall has implications for growth of Gram-positive bacteria. BBA - Biomembranes, 1859(2), doi:10.1016/j.bbamem.2016.12.002. ISSN 0005-2736

Background: The anionic-polyelectrolyte nature of the wall of Gram-positive bacteria has long been suspected to be involved in homeostasis of essential cations and bacterial growth. A better understanding of the coupling between the biophysics and th... Read More

Virulence related sequences; insights provided by comparative genomics of Streptococcus uberis of differing virulence (2015)
Journal Article
Hossain, M., Egan, S. A., Coffey, T., Ward, P. N., Wilson, R., Leigh, J., & Emes, R. (2015). Virulence related sequences; insights provided by comparative genomics of Streptococcus uberis of differing virulence. BMC Genomics, 16, doi:10.1186/s12864-015-1512-6

Background Streptococcus uberis, a Gram-positive, catalase-negative member of the family Streptococcaceae is an important environmental pathogen responsible for a significant proportion of subclinical and clinical bovine intramammary infections. C... Read More

Virulence related sequences: insights provided by comparative genomics of Streptococcus uberis of differing virulence (2015)
Journal Article
Hossain, M., Egan, S. A., Coffey, T., Ward, P. N., Wilson, R., Leigh, J. A., & Emes, R. D. (2015). Virulence related sequences: insights provided by comparative genomics of Streptococcus uberis of differing virulence. BMC Genomics, 16, doi:10.1186/s12864-015-1512-6. ISSN 1471-2164

Background: Streptococcus uberis, a Gram-positive, catalase-negative member of the family Streptococcaceae is an important environmental pathogen responsible for a significant proportion of subclinical and clinical bovine intramammary infections. Cur... Read More

Virulence related sequences; insights provided by comparative genomics of Streptococcus uberis of differing virulence (2015)
Journal Article
Hossain, M., Emes, R., Coffey, T., Egan, S., Ward, P. N., Wilson, R., & Leigh, J. A. (2015). Virulence related sequences; insights provided by comparative genomics of Streptococcus uberis of differing virulence. BMC Genomics, 16, doi:10.1186/s12864-015-1512-6

Background Streptococcus uberis, a Gram-positive, catalase-negative member of the family Streptococcaceae is an important environmental pathogen responsible for a significant proportion of subclinical and clinical bovine intramammary infections. C... Read More

Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System (2015)
Journal Article
Blanchard, A. M., Leigh, J. A., Egan, S. A., & Emes, R. D. (2015). Transposon insertion mapping with PIMMS, Pragmatic Insertional Mutation Mapping System. Frontiers in Genetics, 6, doi:10.3389/fgene.2015.00139. ISSN 1664-8021

The PIMMS (Pragmatic Insertional Mutation Mapping System) pipeline has beendeveloped for simple conditionally essential genome discovery experiments in bacteria.Capable of using raw sequence data files alongside a FASTA sequence of thereference genom... Read More

Arginine dependence of acute myeloid leukaemia blast proliferation: a novel therapeutic target (2015)
Journal Article
Mussai, F., Egan, S. A., Higginbotham-Jones, J., Perry, T., Beggs, A., Odintsova, E., …De Santo, C. (2015). Arginine dependence of acute myeloid leukaemia blast proliferation: a novel therapeutic target. Blood, 125(15), doi:10.1182/blood-2014-09-600643. ISSN 0006-4971

Acute myeloid leukemia (AML) is one of the most common acute leukemias in adults and children, yet significant numbers of patients relapse and die of disease. In this study, we identify the dependence of AML blasts on arginine for proliferation. We s... Read More

Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif (2009)
Journal Article
Egan, S. A., Kurian, D., Ward, P. N., Hunt, L., & Leigh, J. A. (2009). Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif. Journal of Proteome Research, 9(2), doi:10.1021/pr901025w. ISSN 1535-3893

Sortase (a transamidase) has been shown to be responsible for the covalent attachment of proteins to the bacterial cell wall. Anchoring is effected on secreted proteins containing a specific cell wall motif toward their C-terminus; that for sortase A... Read More