Research Repository

See what's under the surface


Characterisation of insulin analogues therapeutically available to patients (2018)
Journal Article
Adams, G. G., Meal, A., Morgan, P. S., Alzahrani, Q. E., Zobel, H., Lithgo, R., …Chayen, N. (2018). Characterisation of insulin analogues therapeutically available to patients. PLoS ONE, 13(3), doi:10.1371/journal.pone.0195010. ISSN 1932-6203

The structure and function of clinical dosage insulin and its analogues were assessed. This included ‘native insulins’ (human recombinant, bovine, porcine), ‘fast-acting analogues’ (aspart, glulisine, lispro) and ‘slow-acting analogues’ (glargine, de... Read More

Glargine and degludec: solution behaviour of higher dose synthetic insulins (2017)
Journal Article
Adams, G. G., Alzahrani, Q., Jiwani, S. I., Meal, A., Morgan, P. S., Coffey, F., …Gillis, R. B. (in press). Glargine and degludec: solution behaviour of higher dose synthetic insulins. Scientific Reports, 7(1), doi:10.1038/s41598-017-06642-w. ISSN 2045-2322

Single, double and triple doses of the synthetic insulins glargine and degludec currently used in patient therapy are characterised using macromolecular hydrodynamic techniques (dynamic light scattering and analytical ultracentrifugation) in an attem... Read More

Genetic improvement of tomato by targeted control of fruit softening (2016)
Journal Article
Uluisik, S., Chapman, N. H., Smith, R., Poole, M., Adams, G., Gillis, R. B., …Seymour, G. B. (2016). Genetic improvement of tomato by targeted control of fruit softening. Nature Biotechnology, 34, 950-952. doi:10.1038/nbt.3602

Controlling the rate of softening to extend shelf life was a key target for researchers engineering genetically modified (GM) tomatoes in the 1990s, but only modest improvements were achieved. Hybrids grown nowadays contain 'non-ripening mutations' t... Read More

Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans (2016)
Journal Article
Gillis, R. B., Adams, G. G., Besong, D. T., Machová, E., Ebringerová, A., Rowe, A. J., …Patel, T. R. (2017). Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans. European Biophysics Journal, 46(3), doi:10.1007/s00249-016-1159-5. ISSN 0175-7571

Polysaccharides, the most abundant biopolymers, are required for a host of activities in lower organisms, animals, and plants. Their solution characterization is challenging due to their complex shape, heterogeneity, and size. Here, recently develope... Read More

A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic (2016)
Journal Article
Gillis, R. B., Adams, G. G., Alzahrani, Q., Harding, S. E., & Allen Bush, C. (2016). A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic. Biopolymers, 105(9), doi:10.1002/bip.22823. ISSN 0006-3525

Under investigation are the structural properties of gum arabic, an industrially important biopolymer for use as a stabilizer or in drug delivery, using Analytical Ultracentrifugation—a well-established, matrix-free probe for macromolecular size and... Read More

Molecular weight analysis of starches: which technique? (2016)
Journal Article
Harding, S. E., Adams, G. G., & Gillis, R. B. (2016). Molecular weight analysis of starches: which technique?. Starch/Stärke, 68(9-10), doi:10.1002/star.201600042. ISSN 0038-9056

A number of methods are available to researchers for estimating molecular weights (molar masses) and molecular weight distributions of starches − or those in solution, using an appropriate solvent/solubilisation protocol. We outline the methods avail... Read More

Coiled coil type neoglycoproteins presenting three lactose residues (2016)
Journal Article
Sweeney, S. M., Bullen, G. A., Gillis, R. B., Adams, G. G., Rowe, A. J., Harding, S. E., …Murphy, P. V. (2016). Coiled coil type neoglycoproteins presenting three lactose residues. Tetrahedron Letters, 57(13), doi:10.1016/j.tetlet.2016.02.005. ISSN 0040-4039

Scaffold design, synthesis and application are relevant for biomedical research. For example, multivalent interactions, such as those between cell surface glycoproteins and lectins can influence the potency and duration of signalling. The spacing bet... Read More

Location dependent coordination chemistry and MRI relaxivity, in de novo designed lanthanide coiled coils (2015)
Journal Article
Berwick, M. R., Slope, L. N., Smith, C. F., King, S. M., Newton, S. L., Gillis, R. B., …Peacock, A. F. A. (in press). Location dependent coordination chemistry and MRI relaxivity, in de novo designed lanthanide coiled coils. Chemical Science, 7(3), doi:10.1039/c5sc04101e. ISSN 2041-6520

Herein, we establish for the first time the design principles for lanthanide coordination within coiled coils, and the important consequences of binding site translation. By interrogating design requirements and by systematically translating binding... Read More

Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins (2015)
Book
Harding, S. E., Adams, G. G., Almutairi, F., Alzahrani, Q., Erten, T., Samil Kök, M., & Gillis, R. B. (2015). Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins. In Analytical UltracentrifugationAcademic Press. doi:10.1016/bs.mie.2015.06.043

Although like proteins, polysaccharides are synthesized by enzymes, unlike proteins there is no template. This means that they are polydisperse, do not generally have compact folded structures, and are often very large with greater nonideality behavi... Read More