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Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes (2023)
Journal Article
Tutt, D. A. R., Guven-Ates, G., Kwong, W. Y., Simmons, R., Sang, F., Silvestri, G., …Sinclair, K. D. (2023). Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes. Frontiers in Endocrinology, 14, Article 1280847. https://doi.org/10.3389/fendo.2023.1280847

Background: In vitro maturation (IVM) of germinal vesicle intact oocytes prior to in vitro fertilization (IVF) is practiced widely in animals. In human assisted reproduction it is generally reserved for fertility preservation or where ovarian stimula... Read More about Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes.

Sexually dimorphic impact of preconceptional and gestational exposure to a real-life environmental chemical mixture (biosolids) on offspring growth dynamics and puberty in sheep (2023)
Journal Article
Evans, N. P., Bellingham, M., Elcombe, C. S., Ghasemzadeh-Hasankolaei, M., Lea, R. G., Sinclair, K. D., & Padmanabhan, V. (2023). Sexually dimorphic impact of preconceptional and gestational exposure to a real-life environmental chemical mixture (biosolids) on offspring growth dynamics and puberty in sheep. Environmental Toxicology and Pharmacology, 102, Article 104257. https://doi.org/10.1016/j.etap.2023.104257

Humans are ubiquitously exposed to complex mixtures of environmental chemicals (ECs). This study characterised changes in post-natal and peripubertal growth, and the activation of the reproductive axis, in male and female offspring of sheep exposed t... Read More about Sexually dimorphic impact of preconceptional and gestational exposure to a real-life environmental chemical mixture (biosolids) on offspring growth dynamics and puberty in sheep.

Developmental exposure to a real-life environmental chemical mixture alters testicular transcription factor expression in neonatal and pre-pubertal rams, with morphological changes persisting into adulthood (2023)
Journal Article
Elcombe, C. S., Monteiro, A., Ghasemzadeh-Hasankolaei, M., Padmanabhan, V., Lea, R., Sinclair, K. D., …Bellingham, M. (2023). Developmental exposure to a real-life environmental chemical mixture alters testicular transcription factor expression in neonatal and pre-pubertal rams, with morphological changes persisting into adulthood. Environmental Toxicology and Pharmacology, 100, Article 104152. https://doi.org/10.1016/j.etap.2023.104152

Environmental chemical (EC) exposure may be impacting male reproductive health. The translationally relevant biosolids treated pasture (BTP) sheep model was used to investigate gestational low-level EC mixture exposure on the testes of F1 male offspr... Read More about Developmental exposure to a real-life environmental chemical mixture alters testicular transcription factor expression in neonatal and pre-pubertal rams, with morphological changes persisting into adulthood.

Enhanced progesterone support during stimulated cycles of transvaginal follicular aspiration improves bovine in vitro embryo production (2023)
Journal Article
Simmons, R., Tutt, D. A., Tutt, D. A., Guven-Ates, G., Kwong, W. Y., Labrecque, R., …Sinclair, K. D. (2023). Enhanced progesterone support during stimulated cycles of transvaginal follicular aspiration improves bovine in vitro embryo production. Theriogenology, 199, 77-85. https://doi.org/10.1016/j.theriogenology.2023.01.003

The in vitro production (IVP) of cattle embryos requires that germinal-vesicle stage oocytes undergo a period of maturation in vitro prior to fertilization and culture to the blastocyst stage. Success of IVP in taurine cattle is enhanced following ov... Read More about Enhanced progesterone support during stimulated cycles of transvaginal follicular aspiration improves bovine in vitro embryo production.

Developmental programming: Preconceptional and gestational exposure of sheep to a real-life environmental chemical mixture alters maternal metabolome in a fetal sex-specific manner (2022)
Journal Article
Thangaraj, S. V., Kachman, M., Halloran, K. M., Sinclair, K. D., Lea, R., Bellingham, M., …Padmanabhan, V. (2023). Developmental programming: Preconceptional and gestational exposure of sheep to a real-life environmental chemical mixture alters maternal metabolome in a fetal sex-specific manner. Science of the Total Environment, 864, Article 161054. https://doi.org/10.1016/j.scitotenv.2022.161054

Background Everyday, humans are exposed to a mixture of environmental chemicals some of which have endocrine and/or metabolism disrupting actions which may contribute to non-communicable diseases. The adverse health impacts of real-world chemical ex... Read More about Developmental programming: Preconceptional and gestational exposure of sheep to a real-life environmental chemical mixture alters maternal metabolome in a fetal sex-specific manner.

Developmental exposure to real-life environmental chemical mixture programs a testicular dysgenesis syndrome-like phenotype in prepubertal lambs (2022)
Journal Article
Elcombe, C. S., Monteiro, A., Elcombe, M. R., Ghasemzadeh-Hasankolaei, M., Sinclair, K. D., Lea, R., …Bellingham, M. (2022). Developmental exposure to real-life environmental chemical mixture programs a testicular dysgenesis syndrome-like phenotype in prepubertal lambs. Environmental Toxicology and Pharmacology, 94, Article 103913. https://doi.org/10.1016/j.etap.2022.103913

Current declines in male reproductive health may, in part, be driven by anthropogenic environmental chemical (EC) exposure. Using a biosolids treated pasture (BTP) sheep model, this study examined the effects of gestational exposure to a translationa... Read More about Developmental exposure to real-life environmental chemical mixture programs a testicular dysgenesis syndrome-like phenotype in prepubertal lambs.

Effects of single or serial embryo splitting on the development and morphokinetics of in vitro produced bovine embryos (2022)
Journal Article
Silvestri, G., Turner, K. J., Silcock, J. L., Sinclair, K. D., & Griffin, D. K. (2022). Effects of single or serial embryo splitting on the development and morphokinetics of in vitro produced bovine embryos. The European Zoological Journal, 89(1), 680-689. https://doi.org/10.1080/24750263.2022.2077994

Embryo splitting can be used in cattle in vitro production (IVP) to improve embryo availability and to increase selection intensity. Despite this widespread utility, a comparative investigation of the viability of IVP embryos split at Day 2 (2-cell s... Read More about Effects of single or serial embryo splitting on the development and morphokinetics of in vitro produced bovine embryos.

Maternal obesity during pregnancy leads to derangements in one-carbon metabolism and the gut microbiota: implications for fetal development and offspring wellbeing (2022)
Journal Article
Rubini, E., Schenkelaars, N., Rousian, M., Sinclair, K. D., Wekema, L., Faas, M. M., …Schoenmakers, S. (2022). Maternal obesity during pregnancy leads to derangements in one-carbon metabolism and the gut microbiota: implications for fetal development and offspring wellbeing. American Journal of Obstetrics and Gynecology, 227(3), 392-400. https://doi.org/10.1016/j.ajog.2022.04.013

A healthy diet prior to and during pregnancy is beneficial in acquiring essential B vitamins involved in one-carbon metabolism, and to maintain a healthy gut microbiota. Each play important roles in fetal development, immune-system remodeling and pre... Read More about Maternal obesity during pregnancy leads to derangements in one-carbon metabolism and the gut microbiota: implications for fetal development and offspring wellbeing.

First Trimester Maternal Homocysteine and Embryonic and Fetal Growth: The Rotterdam Periconception Cohort (2022)
Journal Article
Rubini, E., Snoek, K. M., Schoenmakers, S., Willemsen, S. P., Sinclair, K. D., Rousian, M., & Steegers-Theunissen, R. P. (2022). First Trimester Maternal Homocysteine and Embryonic and Fetal Growth: The Rotterdam Periconception Cohort. Nutrients, 14(6), Article 1129. https://doi.org/10.3390/nu14061129

Homocysteine is a marker for derangements in one-carbon metabolism. Elevated homocysteine may represent a causal link between poor maternal nutrition and impaired embryonic and fetal development. We sought to investigate associations between referenc... Read More about First Trimester Maternal Homocysteine and Embryonic and Fetal Growth: The Rotterdam Periconception Cohort.

Ovine fetal testis stage-specific sensitivity to environmental chemical mixtures (2022)
Journal Article
Lea, R. G., Mandon-Pépin, B., Loup, B., Poumerol, E., Jouneau, L., Egbowon, B. F., …Sinclair, K. D. (2022). Ovine fetal testis stage-specific sensitivity to environmental chemical mixtures. Reproduction, 163(2), 119-131. https://doi.org/10.1530/REP-21-0235

Exposure of the fetal testis to numerous individual environmental chemicals (ECs) is frequently associated with dysregulated development, leading to impaired adult reproductive competence. However, 'real-life' exposure involves complex mixtures of EC... Read More about Ovine fetal testis stage-specific sensitivity to environmental chemical mixtures.

Maternal one-carbon metabolism during the periconceptional period and human foetal brain growth: A systematic review (2021)
Journal Article
Rubini, E., Baijens, I. M., Horánszky, A., Schoenmakers, S., Sinclair, K. D., Zana, M., …Rousian, M. (2021). Maternal one-carbon metabolism during the periconceptional period and human foetal brain growth: A systematic review. Genes, 12(10), Article 1634. https://doi.org/10.3390/genes12101634

The maternal environment during the periconceptional period influences foetal growth and development, in part, via epigenetic mechanisms moderated by one-carbon metabolic pathways. During embryonic development, one-carbon metabolism is involved in br... Read More about Maternal one-carbon metabolism during the periconceptional period and human foetal brain growth: A systematic review.

Preimplantation genetic testing for aneuploidy improves live birth rates with in vitro produced bovine embryos: A blind retrospective study (2021)
Journal Article
Silvestri, G., Canedo-Ribeiro, C., Serrano-Albal, M., Labrecque, R., Blondin, P., Larmer, S. G., …Griffin, D. K. (2021). Preimplantation genetic testing for aneuploidy improves live birth rates with in vitro produced bovine embryos: A blind retrospective study. Cells, 10(9), Article 2284. https://doi.org/10.3390/cells10092284

Approximately one million in vitro produced (IVP) cattle embryos are transferred world-wide each year as a way to improve the rates of genetic gain. The most advanced programmes also apply genomic selection at the embryonic stage by SNP genotyping an... Read More about Preimplantation genetic testing for aneuploidy improves live birth rates with in vitro produced bovine embryos: A blind retrospective study.

Interspecific Variation in One-Carbon Metabolism within the Ovarian Follicle, Oocyte, and Preimplantation Embryo: Consequences for Epigenetic Programming of DNA Methylation (2021)
Journal Article
Clare, C. E., Pestinger, V., Kwong, W. Y., Tutt, D. A. R., Xu, J., Byrne, H. M., …Sinclair, K. D. (2021). Interspecific Variation in One-Carbon Metabolism within the Ovarian Follicle, Oocyte, and Preimplantation Embryo: Consequences for Epigenetic Programming of DNA Methylation. International Journal of Molecular Sciences, 22(4), Article 1838. https://doi.org/10.3390/ijms22041838

One-carbon (1C) metabolism provides methyl groups for the synthesis and/or methylation 20 of purines and pyrimidines, biogenic amines, proteins and phospholipids. Our understanding of 21 how 1C pathways operate, however, pertains mostly to the (rat)... Read More about Interspecific Variation in One-Carbon Metabolism within the Ovarian Follicle, Oocyte, and Preimplantation Embryo: Consequences for Epigenetic Programming of DNA Methylation.

Analysis of bovine blastocysts indicates ovarian stimulation does not induce chromosome errors, nor discordance between inner-cell mass and trophectoderm lineages (2020)
Journal Article
Tutt, D. A., Silvestri, G., Serrano-Albal, M., Simmons, R. J., Kwong, W. Y., Guven-Ates, G., …Sinclair, K. D. (2021). Analysis of bovine blastocysts indicates ovarian stimulation does not induce chromosome errors, nor discordance between inner-cell mass and trophectoderm lineages. Theriogenology, 161, 108-119. https://doi.org/10.1016/j.theriogenology.2020.11.021

© 2020 The Authors Contemporary systems for oocyte retrieval and culture of both cattle and human embryos are suboptimal with respect to pregnancy outcomes following transfer. In humans, chromosome abnormalities are the leading cause of early pregnan... Read More about Analysis of bovine blastocysts indicates ovarian stimulation does not induce chromosome errors, nor discordance between inner-cell mass and trophectoderm lineages.

Polycystic ovary syndrome: A brain disorder characterized by eating problems originating during puberty and adolescence (2020)
Journal Article
Steegers-Theunissen, R. P. M., Wiegel, R. E., Jansen, P. W., Laven, J. S. E., & Sinclair, K. D. (2020). Polycystic ovary syndrome: A brain disorder characterized by eating problems originating during puberty and adolescence. International Journal of Molecular Sciences, 21(21), Article 8211. https://doi.org/10.3390/ijms21218211

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Polycystic ovary syndrome (PCOS) is an endocrine condition associated with reproductive and psychiatric disorders, and with obesity. Eating disorders, such as bulimia and recurrent dieting, ar... Read More about Polycystic ovary syndrome: A brain disorder characterized by eating problems originating during puberty and adolescence.

Comprehensive and quantitative profiling of B vitamins and related compounds in the mammalian liver (2019)
Journal Article
Xu, J., Clare, C. E., Brassington, A. H., Sinclair, K., & Barrett, D. (2020). Comprehensive and quantitative profiling of B vitamins and related compounds in the mammalian liver. Journal of Chromatography B, 1136, https://doi.org/10.1016/j.jchromb.2019.121884

A method for the simultaneous quantification of B vitamins and related amines in one-carbon (1C) metabolism would benefit the study of diet and genetic/epigenetic regulation of mammalian development and health. We present a validated method for the s... Read More about Comprehensive and quantitative profiling of B vitamins and related compounds in the mammalian liver.

Karyomapping for simultaneous genomic evaluation and aneuploidy screening of preimplantation bovine embryos: The first live-born calves (2018)
Journal Article
Handyside, A. H., Turner, K. J., Silvestri, G., Black, D. H., Dobson, G., Smith, C., …Griffin, D. K. (2019). Karyomapping for simultaneous genomic evaluation and aneuploidy screening of preimplantation bovine embryos: The first live-born calves. Theriogenology, 125, 249-258. https://doi.org/10.1016/j.theriogenology.2018.11.014

© 2018 The Authors In cattle breeding, the development of genomic selection strategies based on single nucleotide polymorphism (SNP) interrogation has led to improved rates of genetic gain. Additionally, the application of genomic selection to in-vit... Read More about Karyomapping for simultaneous genomic evaluation and aneuploidy screening of preimplantation bovine embryos: The first live-born calves.

One-Carbon Metabolism: Linking Nutritional Biochemistry to Epigenetic Programming of Long-Term Development (2018)
Journal Article
Clare, C. E., Brassington, A. H., Kwong, W. Y., & Sinclair, K. D. (2019). One-Carbon Metabolism: Linking Nutritional Biochemistry to Epigenetic Programming of Long-Term Development. Annual Review of Animal Biosciences, 7(1), 263-287. https://doi.org/10.1146/annurev-animal-020518-115206

One-carbon (1C) metabolism comprises a series of interlinking metabolic pathways that include the methionine and folate cycles that are central to cellular function, providing 1C units (methyl groups) for the synthesis of DNA, polyamines, amino acids... Read More about One-Carbon Metabolism: Linking Nutritional Biochemistry to Epigenetic Programming of Long-Term Development.

Added dietary cobalt or vitamin B12, or injecting vitamin B12 does not improve performance or indicators of ketosis in pre- and post-partum Holstein-Friesian dairy cows (2018)
Journal Article
Weerathilake, W. A. D. V., Brassington, A. H., Williams, S. J., Kwong, W. Y., Sinclair, L. A., & Sinclair, K. D. (2019). Added dietary cobalt or vitamin B12, or injecting vitamin B12 does not improve performance or indicators of ketosis in pre- and post-partum Holstein-Friesian dairy cows. animal, 13(4), 750-759. https://doi.org/10.1017/S175173111800232X

Vitamin B12 is synthesised in the rumen from cobalt (Co) and has a major role in metabolism in the peri-paturient period, although few studies have evaluated the effect of the dietary inclusion of Co, vitamin B12 or injecting vitamin B12 on the metab... Read More about Added dietary cobalt or vitamin B12, or injecting vitamin B12 does not improve performance or indicators of ketosis in pre- and post-partum Holstein-Friesian dairy cows.