DNA methylation changes during long-term in vitro cell culture are caused by epigenetic drift
(2021)
Journal Article
Franzen, J., Georgomanolis, T., Selich, A., Kuo, C., Stöger, R., Brant, L., …Wagner, W. (2021). DNA methylation changes during long-term in vitro cell culture are caused by epigenetic drift. Communications Biology, 4(1), Article 598. https://doi.org/10.1038/s42003-021-02116-y
Culture expansion of primary cells evokes highly reproducible DNA methylation (DNAm) changes. We have identified CG dinucleotides (CpGs) that become continuously hyper- or hypomethylated during long-term culture of mesenchymal stem cells (MSCs) and o... Read More about DNA methylation changes during long-term in vitro cell culture are caused by epigenetic drift.