Use of the Affymetrix human GeneChip array and genomic DNA hybridisation probe selection to study ovine transcriptomes

Graham, N. S.; May, S. T.; Daniel, Z. C.T.R.; Emmerson, Z. F.; Brameld, J. M.; Parr, T.

doi:10.1017/S1751731110002533

Sodium hyperaccumulators in the Caryophyllales are characterized by both abnormally large shoot sodium concentrations and [Na]shoot/[Na]root quotients greater than unity (2021)
Journal Article
Neugebauer, K., Broadley, M. R., El-Serehy, H. A., George, T. S., Graham, N. S., Thompson, J. A., …White, P. J. (2022). Sodium hyperaccumulators in the Caryophyllales are characterized by both abnormally large shoot sodium concentrations and [Na]shoot/[Na]root quotients greater than unity. Annals of Botany, 129(1), 65-78. https://doi.org/10.1093/aob/mcab126

BACKGROUND AND AIMS: Some Caryophyllales species accumulate abnormally large shoot sodium (Na) concentrations in non-saline environments. It is not known whether this is a consequence of altered Na partitioning between roots and shoots. This paper te... Read More about Sodium hyperaccumulators in the Caryophyllales are characterized by both abnormally large shoot sodium concentrations and [Na]shoot/[Na]root quotients greater than unity.

Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa (2021)
Journal Article
Alcock, T. D., Thomas, C. L., Ó Lochlainn, S., Pongrac, P., Wilson, M., Moore, C., …Broadley, M. R. (2021). Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa. Plant Physiology, 186(3), 1616-1631. https://doi.org/10.1093/PLPHYS/KIAB150

Magnesium (Mg) and calcium (Ca) are essential mineral nutrients poorly supplied in many human food systems. In grazing livestock, Mg and Ca deficiencies are costly welfare issues. Here, we report a Brassica rapa loss-of-function schengen3 (sgn3) muta... Read More about Magnesium and calcium overaccumulate in the leaves of a schengen3 mutant of Brassica rapa.

Magnesium biofortification of Italian ryegrass (Lolium multiflorum L.) via agronomy and breeding as a potential way to reduce grass tetany in grazing ruminants (2019)
Journal Article
Kumssa, D. B., Lovatt, J. A., Graham, N. S., Palmer, S., Hayden, R., Wilson, L., …Broadley, M. R. (2020). Magnesium biofortification of Italian ryegrass (Lolium multiflorum L.) via agronomy and breeding as a potential way to reduce grass tetany in grazing ruminants. Plant and Soil, 457, 25–41. https://doi.org/10.1007/s11104-019-04337-x

© 2019, The Author(s). Aim: Magnesium (Mg) deficiency (known as grass tetany) is a serious metabolic disorder that affects grazing ruminants. We tested whether Mg-fertiliser can increase Mg concentration of Italian ryegrasses (Lolium multiflorum L.)... Read More about Magnesium biofortification of Italian ryegrass (Lolium multiflorum L.) via agronomy and breeding as a potential way to reduce grass tetany in grazing ruminants.

Uptake competition between selenium and sulphur fertilizers in sequential harvests of ryegrass: A stable isotope study (2019)
Conference Proceeding
Jiang, L., Young, S. D., Bailey, E. H., Broadley, M. R., Graham, N. S., & McGrath, S. P. (2019). Uptake competition between selenium and sulphur fertilizers in sequential harvests of ryegrass: A stable isotope study. In G. Bañuelos, Z. Lin, D. Liang, & X. Yin (Eds.), Selenium Research for Environment and Human Health: Perspectives, Technologies and Advancements - Proceedings of the 6th International Conference on Selenium in the Environment and Human Health, 2019 (57-58). https://doi.org/10.1201/9780429423482-27

The demand for sulphur (S) fertilizers has increased due to declining S emissions from coal burning and subsequent deposition on agricultural land. Slow-release S may only be beneficial for grass Selenium (Se) status initially and less beneficial for... Read More about Uptake competition between selenium and sulphur fertilizers in sequential harvests of ryegrass: A stable isotope study.

Overexpression of the MYB29 transcription factor affects aliphatic glucosinolate synthesis in Brassica oleracea (2019)
Journal Article
Zuluaga, D. L., Graham, N. S., Klinder, A., van Ommen Kloeke, A. E., Marcotrigiano, A. R., Wagstaff, C., …Aarts, M. G. (2019). Overexpression of the MYB29 transcription factor affects aliphatic glucosinolate synthesis in Brassica oleracea. Plant Molecular Biology, 101(1-2), 65–79. https://doi.org/10.1007/s11103-019-00890-2

Isothiocyanates, the bio-active hydrolysis products of glucosinolates, naturally produced by several Brassicaceae species, play an important role in human health and agriculture. This study aims at correlating the content of aliphatic glucosinolates... Read More about Overexpression of the MYB29 transcription factor affects aliphatic glucosinolate synthesis in Brassica oleracea.

Species-wide variation in shoot nitrate concentration, and genetic loci controlling nitrate, phosphorous and potassium accumulation in Brassica napus L (2018)
Journal Article
Alcock, T. D., Havlickova, L., He, Z., Wilson, L., Bancroft, I., White, P. J., …Graham, N. S. (2018). Species-wide variation in shoot nitrate concentration, and genetic loci controlling nitrate, phosphorous and potassium accumulation in Brassica napus L. Frontiers in Plant Science, 9, Article 1487. https://doi.org/10.3389/fpls.2018.01487

A PSTOL-like gene, TaPSTOL, controls a number of agronomically important traits in wheat (2018)
Journal Article
Milner, M. J., Howells, R. M., Craze, M., Bowden, S., Graham, N., & Wallington, E. J. (2018). A PSTOL-like gene, TaPSTOL, controls a number of agronomically important traits in wheat. BMC Plant Biology, 18(1), Article 115. https://doi.org/10.1186/s12870-018-1331-4

Background Phosphorus (P) is an essential macronutrient for plant growth, and is required in large quantities by elite varieties of crops to maintain yields. Approximately 70% of global cultivated land suffers from P deficiency, and it has recentl... Read More about A PSTOL-like gene, TaPSTOL, controls a number of agronomically important traits in wheat.

Physiological profile of CAX1a TILLING mutants of Brassica rapa exposed to different calcium doses (2018)
Journal Article
Navarro-León, E., Ruiz, J. M., Graham, N., & Blasco, B. (in press). Physiological profile of CAX1a TILLING mutants of Brassica rapa exposed to different calcium doses. Plant Science, https://doi.org/10.1016/j.plantsci.2018.04.019

Calcium (Ca) is an essential macronutrient for plants and its homeostasis is basic for many processes in plants. Therefore, both Ca deficiency and toxicity constitute potential issues for crops. CAX1 transporter is a potential target to obtain plants... Read More about Physiological profile of CAX1a TILLING mutants of Brassica rapa exposed to different calcium doses.

Identification of candidate genes for calcium and magnesium accumulation in Brassica napus L. by association genetics (2017)
Journal Article
Alcock, T. D., Havlickova, L., He, Z., Bancroft, I., White, P. J., Broadley, M. R., & Graham, N. S. (in press). Identification of candidate genes for calcium and magnesium accumulation in Brassica napus L. by association genetics. Frontiers in Plant Science, 8, Article 2081. https://doi.org/10.3389/fpls.2017.01968

Calcium (Ca) and magnesium (Mg) are essential plant nutrients and vital for human and animal nutrition. Biofortification of crops has previously been suggested to alleviate widespread human Ca and Mg deficiencies. In this study, new candidate genes i... Read More about Identification of candidate genes for calcium and magnesium accumulation in Brassica napus L. by association genetics.

Development of high-throughput methods to screen disease caused by Rhizoctonia solani AG 2-1 in oilseed rape (2017)
Journal Article
Drizou, F., Graham, N. S., Bruce, T. J. A., & Ray, R. V. (2017). Development of high-throughput methods to screen disease caused by Rhizoctonia solani AG 2-1 in oilseed rape. Plant Methods, 13(1), https://doi.org/10.1186/s13007-017-0195-1

Background: Rhizoctonia solani (Kühn) is a soil-borne, necrotrophic fungus causing damping off, root rot and stem canker in many cultivated plants worldwide. Oilseed rape (OSR, Brassica napus) is the primary host for anastomosis group (AG) 2-1 of R.... Read More about Development of high-throughput methods to screen disease caused by Rhizoctonia solani AG 2-1 in oilseed rape.

Root morphology and seed and leaf ionomic traits in a Brassica napus L. diversity panel show wide phenotypic variation and are characteristic of crop habit (2016)
Journal Article
Thomas, C., Alcock, T., Graham, N., Hayden, R., Matterson, S., Wilson, L., …Broadley, M. R. (2016). Root morphology and seed and leaf ionomic traits in a Brassica napus L. diversity panel show wide phenotypic variation and are characteristic of crop habit. BMC Plant Biology, 16(1), Article 214. https://doi.org/10.1186/s12870-016-0902-5

Background Mineral nutrient uptake and utilisation by plants are controlled by many traits relating to root morphology, ion transport, sequestration and translocation. The aims of this study were to determine the phenotypic diversity in root morph... Read More about Root morphology and seed and leaf ionomic traits in a Brassica napus L. diversity panel show wide phenotypic variation and are characteristic of crop habit.

QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems (2016)
Journal Article
Zhang, Y., Thomas, C. L., Xiang, J., Long, Y., Wang, X., Zou, J., …Meng, J. (2016). QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems. Scientific Reports, 6(1), Article 33113. https://doi.org/10.1038/srep33113

© The Author(s) 2016. A high-density SNP-based genetic linkage map was constructed and integrated with a previous map in the Tapidor x Ningyou7 (TNDH) Brassica napus population, giving a new map with a total of 2041 molecular markers and an average m... Read More about QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems.

High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.) (2016)
Journal Article
Thomas, C. L., Graham, N. .., Hayden, R., Meacham, M. C., Neugebauer, K., Nightingale, M., …Broadley, M. R. (in press). High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.). Annals of Botany, 118(4), https://doi.org/10.1093/aob/mcw046

Background and Aims. Root traits can be selected for crop improvement. Techniques such as soil excavations can be used to screen root traits in the field, but are limited to genotypes that are well-adapted to field conditions. The aim of this study w... Read More about High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.).

Genetical and comparative genomics of Brassica under altered Ca supply identifies Arabidopsis Ca-transporter orthologs (2014)
Journal Article
Graham, N. S., Hammond, J. P., Lysenko, A., Mayes, S., Ó Lochlainn, S., Blasco, B., …Broadley, M. R. (in press). Genetical and comparative genomics of Brassica under altered Ca supply identifies Arabidopsis Ca-transporter orthologs. Plant Cell, 26(7),

Although Ca transport in plants is highly complex, the overexpression of vacuolar Ca2+ transporters in crops is a promising new technology to improve dietary Ca supplies through biofortification. Here, we sought to identify novel targets for increasi... Read More about Genetical and comparative genomics of Brassica under altered Ca supply identifies Arabidopsis Ca-transporter orthologs.

Use of the Affymetrix human GeneChip array and genomic DNA hybridisation probe selection to study ovine transcriptomes (2011)
Journal Article
Graham, N. S., May, S. T., Daniel, Z. C., Emmerson, Z. F., Brameld, J. M., & Parr, T. (2011). Use of the Affymetrix human GeneChip array and genomic DNA hybridisation probe selection to study ovine transcriptomes. animal, 5(6), 861-866. https://doi.org/10.1017/S1751731110002533

Affymetrix GeneChip microarrays are a powerful tool to study global gene expression profiles and have been used on many species. However, no sheep-specific Affymetrix GeneChip is currently available and the bovine array is fairly limited in its cover... Read More about Use of the Affymetrix human GeneChip array and genomic DNA hybridisation probe selection to study ovine transcriptomes.

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