Research Repository

See what's under the surface


Functional genetic elements for controlling gene expression in Cupriavidus necator H16 (2018)
Journal Article
Alagesan, S., Hanko, E. K., Malys, N., Ehsaan, M., Winzer, K., & Minton, N. P. (2018). Functional genetic elements for controlling gene expression in Cupriavidus necator H16. Applied and Environmental Microbiology, doi:10.1128/AEM.00878-18. ISSN 0099-2240

A robust and predictable control of gene expression plays an important role in synthetic biology and biotechnology applications. Development and quantitative evaluation of functional genetic elements such as constitutive and inducible promoters, as w... Read More

A roadmap for gene system development in Clostridium (2016)
Journal Article
Minton, N. P., Ehsaan, M., Humphreys, C. M., Little, G. T., Baker, J., Henstra, A. M., …Zhang, Y. (2016). A roadmap for gene system development in Clostridium. Anaerobe, 41, doi:10.1016/j.anaerobe.2016.05.011. ISSN 1075-9964

Clostridium species are both heroes and villains. Some cause serious human and animal diseases, those present in the microbiota contribute to health and wellbeing, while others represent useful industrial chassis for the production of chemicals and f... Read More

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker (2012)
Journal Article
Heap, J. T., Ehsaan, M., Cooksley, C. M., Ng, Y., Cartman, S. T., Winzer, K., & Minton, N. P. (2012). Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker. Nucleic Acids Research, 40(8), doi:10.1093/nar/gkr1321. ISSN 0305-1048

Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection of double-crossover clones. To overcome the limited availability of... Read More