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Oxygen-dependent proteolysis regulates the stability of angiosperm polycomb repressive complex 2 subunit VERNALIZATION2 (2018)
Journal Article
Gibbs, D. J., Tedds, H. M., Labandera, A., Bailey, M., White, M. D., Hartman, S., …Holdsworth, M. J. (2018). Oxygen-dependent proteolysis regulates the stability of angiosperm polycomb repressive complex 2 subunit VERNALIZATION2. Nature Communications, 9, doi:10.1038/s41467-018-07875-7

The polycomb repressive complex 2 (PRC2) regulates epigenetic gene repression in eukaryotes. Mechanisms controlling its developmental specificity and signal-responsiveness are poorly understood. Here, we identify an oxygen-sensitive N-terminal (N-) d... Read More

Genetic interactions between ABA signalling and the Arg/N-end rule pathway during Arabidopsis seedling establishment (2018)
Journal Article
Zhang, H., Gannon, L., Jones, P. D., Rundle, C. A., Hassall, K. L., Gibbs, D. J., …Theodoulou, F. L. (2018). Genetic interactions between ABA signalling and the Arg/N-end rule pathway during Arabidopsis seedling establishment. Scientific Reports, 8(1), doi:10.1038/s41598-018-33630-5

The Arg/N-end rule pathway of ubiquitin-mediated proteolysis has multiple functions throughout plant development, notably in the transition from dormant seed to photoautotrophic seedling. PROTEOLYSIS6 (PRT6), an N-recognin E3 ligase of the Arg/N-end... Read More

Distinct branches of the N-end rule pathway modulate the plant immune response (2018)
Journal Article
Vicente, J., Mendiondo, G. M., Pauwels, J., Pastor, V., Izquierdo, Y., Naumann, C., …Holdsworth, M. J. (2018). Distinct branches of the N-end rule pathway modulate the plant immune response. New Phytologist, doi:10.1111/nph.15387. ISSN 0028-646X

The N‐end rule pathway is a highly conserved constituent of the ubiquitin proteasome system, yet little is known about its biological roles. Here we explored the role of the N‐end rule pathway in the plant immune response. We investigated the geneti... Read More

The Cys-Arg/N-end rule pathway is a general sensor of abiotic stress in flowering plants (2017)
Journal Article
Vicente, J., Mendiondo, G. M., Movahedi, M., Peirats-Llobet, M., Juan, Y., Shen, Y., …Holdsworth, M. J. (2017). The Cys-Arg/N-end rule pathway is a general sensor of abiotic stress in flowering plants. Current Biology, 27(20), doi:10.1016/j.cub.2017.09.006

Abiotic stresses impact negatively on plant growth, profoundly affecting yield and quality of crops. Although much is known about plant responses, very little is understood at the molecular level about the initial sensing of environmental stress. In... Read More

Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot (2016)
Journal Article
Gravot, A., Richard, G., Lime, T., Lemarié, S., Jubault, M., Lariagon, C., …Manzanares-Dauleux, M. J. (2016). Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot. BMC Plant Biology, 16, doi:10.1186/s12870-016-0941-y. ISSN 1471-2229

Background The induction of alcohol fermentation in roots is a plant adaptive response to flooding stress and oxygen deprivation. Available transcriptomic data suggest that fermentation-related genes are also frequently induced in roots infected w... Read More

From start to finish: amino-terminal protein modifications as degradation signals in plants (2016)
Journal Article
Gibbs, D. J., Bailey, M., Tedds, H. M., & Holdsworth, M. J. (2016). From start to finish: amino-terminal protein modifications as degradation signals in plants. New Phytologist, 211(4), 1188-1194. doi:10.1111/nph.14105

The amino- (N-) terminus (Nt) of a protein can undergo a diverse array of co- and posttranslational modifications. Many of these create degradation signals (N-degrons) that mediate protein destruction via the N-end rule pathway of ubiquitin-mediated... Read More

Group VII ethylene response factors coordinate oxygen and nitric oxide signal transduction and stress responses in plants (2015)
Journal Article
Gibbs, D. J., Vincente Conde, J., Berckhan, S., Prasad, G., Mendiondo, G. M., & Holdsworth, M. J. (2015). Group VII ethylene response factors coordinate oxygen and nitric oxide signal transduction and stress responses in plants. Plant Physiology, 169(1), doi:10.1104/pp.15.00338

The group VII ethylene response factors (ERFVIIs) are plant-specific transcription factors that have emerged as important regulators of abiotic and biotic stress responses, in particular, low-oxygen stress. A defining feature of ERFVIIs is their cons... Read More

Nitric oxide sensing in plants is mediated by proteolytic control of Group VII ERF transcription factors (2014)
Journal Article
Gibbs, D. J., Md Isa, N., Movahedi, M., Lozano-Juste, J., Mendiondo, G. M., Berckhan, S., …Holdsworth, M. J. (2014). Nitric oxide sensing in plants is mediated by proteolytic control of Group VII ERF transcription factors. Molecular Cell, 53(3), doi:10.1016/j.molcel.2013.12.020

Nitric oxide (NO) is an important signaling compound in prokaryotes and eukaryotes. In plants, NO regulates critical developmental transitions and stress responses. Here, we identify a mechanism for NO sensing that coordinates responses throughout de... Read More