All Outputs (23)

ERFVII action and modulation through oxygen-sensing in Arabidopsis thaliana (2023)
Journal Article
Zubrycka, A., Dambire, C., Dalle Carbonare, L., Sharma, G., Boeckx, T., Swarup, K., …Holdsworth, M. J. (2023). ERFVII action and modulation through oxygen-sensing in Arabidopsis thaliana. Nature Communications, 14, Article 4665. https://doi.org/10.1038/s41467-023-40366-y

Oxygen is a key signalling component of plant biology, and whilst an oxygen-sensing mechanism was previously described in Arabidopsis thaliana, key features of the associated PLANT CYSTEINE OXIDASE (PCO) N-degron pathway and Group VII ETHYLENE RESPON... Read More about ERFVII action and modulation through oxygen-sensing in Arabidopsis thaliana.

A phloem‐localized Arabidopsis metacaspase (AtMC3) improves drought tolerance (2023)
Journal Article
Pitsili, E., Rodriguez‐Trevino, R., Ruiz‐Solani, N., Demir, F., Kastanaki, E., Dambire, C., …Coll, N. S. (2023). A phloem‐localized Arabidopsis metacaspase (AtMC3) improves drought tolerance. New Phytologist, https://doi.org/10.1111/nph.19022

Increasing drought phenomena pose a serious threat to agricultural productivity. Although plants have multiple ways to respond to the complexity of drought stress, the underlying mechanisms of stress sensing and signaling remain unclear. The role of... Read More about A phloem‐localized Arabidopsis metacaspase (AtMC3) improves drought tolerance.

An oxygen-sensing mechanism for angiosperm adaptation to altitude (2022)
Journal Article
Abbas, M., Sharma, G., Dambire, C., Marquez, J., Alonso-Blanco, C., Proaño, K., & Holdsworth, M. J. (2022). An oxygen-sensing mechanism for angiosperm adaptation to altitude. Nature, https://doi.org/10.1038/s41586-022-04740-y

Flowering plants (angiosperms) can grow at extreme altitudes, and have been observed growing as high as 6,400 metres above sea level1,2; however, the molecular mechanisms that enable plant adaptation specifically to altitude are unknown. One distingu... Read More about An oxygen-sensing mechanism for angiosperm adaptation to altitude.

Allelic shift in cis-elements of the transcription factor RAP2.12 underlies adaptation associated with humidity in Arabidopsis thaliana (2022)
Journal Article
Lou, S., Guo, X., Liu, L., Song, Y., Zhang, L., Jiang, Y., …Liu, J. (2022). Allelic shift in cis-elements of the transcription factor RAP2.12 underlies adaptation associated with humidity in Arabidopsis thaliana. Science Advances, 8(18), Article eabn8281. https://doi.org/10.1126/sciadv.abn8281

Populations of widespread species are usually geographically distributed through contrasting stresses, but underlying genetic mechanisms controlling this adaptation remain largely unknown. Here, we show that in Arabidopsis thaliana, allelic changes i... Read More about Allelic shift in cis-elements of the transcription factor RAP2.12 underlies adaptation associated with humidity in Arabidopsis thaliana.

Mitochondrial retrograde signaling through UCP1-mediated inhibition of the plant oxygen-sensing pathway (2022)
Journal Article
Barreto, P., Dambire, C., Sharma, G., Vicente, J., Osborne, R., De Carvalho, J. E., …Arruda, P. (2022). Mitochondrial retrograde signaling through UCP1-mediated inhibition of the plant oxygen-sensing pathway. Current Biology, 32(6), 1403-1411.e4. https://doi.org/10.1016/j.cub.2022.01.037

Mitochondrial retrograde signaling is an important component of intracellular stress signaling in eukaryotes. UNCOUPLING PROTEIN (UCP)1 is an abundant plant inner-mitochondrial membrane protein with multiple functions including uncoupled respiration... Read More about Mitochondrial retrograde signaling through UCP1-mediated inhibition of the plant oxygen-sensing pathway.

A Yeast-Based Functional Assay to Study Plant N-Degron – N-Recognin Interactions (2022)
Journal Article
Kozlic, A., Winter, N., Telser, T., Reimann, J., Rose, K., Nehlin, L., …Bachmair, A. (2022). A Yeast-Based Functional Assay to Study Plant N-Degron – N-Recognin Interactions. Frontiers in Plant Science, 12, Article 806129. https://doi.org/10.3389/fpls.2021.806129

The N-degron pathway is a branch of the ubiquitin-proteasome system where amino-terminal residues serve as degradation signals. In a synthetic biology approach, we expressed ubiquitin ligase PRT6 and ubiquitin conjugating enzyme 2 (AtUBC2) from Arabi... Read More about A Yeast-Based Functional Assay to Study Plant N-Degron – N-Recognin Interactions.

Comparative Biology of Oxygen Sensing in Plants and Animals (2020)
Journal Article
Gibbs, D. J., & Holdsworth, M. J. (2020). Comparative Biology of Oxygen Sensing in Plants and Animals. Current Biology, 30(8), R362-R369. https://doi.org/10.1016/j.cub.2020.03.021

© 2020 Elsevier Inc. Aerobic respiration is essential to almost all eukaryotes and sensing oxygen is a key determinant of survival. Analogous but mechanistically different oxygen-sensing pathways were adopted in plants and metazoan animals, and inclu... Read More about Comparative Biology of Oxygen Sensing in Plants and Animals.

The plant N?degron pathways of ubiquitin?mediated proteolysis (2019)
Journal Article
Holdsworth, M. J., Vicente, J., Sharma, G., Abbas, M., & Zubrycka, A. (2020). The plant N?degron pathways of ubiquitin?mediated proteolysis. Journal of Integrative Plant Biology, 62(1), 70-89. https://doi.org/10.1111/jipb.12882

The amino?terminal residue of a protein (or amino?terminus of a peptide following protease cleavage) can be an important determinant of its stability, through the Ubiquitin?Proteasome?System associated N?degron pathways. Plants contain a unique combi... Read More about The plant N?degron pathways of ubiquitin?mediated proteolysis.

Ethylene-mediated nitric oxide depletion pre-adapts plants to hypoxia stress (2019)
Journal Article
Hartman, S., Liu, Z., Van Veen, H., Vicente, J., Reinen, E., Martopawiro, S., …Voesenek, L. A. (2019). Ethylene-mediated nitric oxide depletion pre-adapts plants to hypoxia stress. Nature Communications, 10, Article 4020. https://doi.org/10.1038/s41467-019-12045-4

Timely perception of adverse environmental changes is critical for survival. Dynamic changes in gases are important cues for plants to sense environmental perturbations, such as submergence. In Arabidopsis thaliana, changes in oxygen and nitric oxide... Read More about Ethylene-mediated nitric oxide depletion pre-adapts plants to hypoxia stress.

N-terminomics reveals control of Arabidopsis seed storage proteins and proteases by the Arg/N-end rule pathway (2017)
Journal Article
Zhang, H., Gannon, L., Hassall, K. L., Deery, M. J., Gibbs, D. J., Holdsworth, M. J., …Theodoulou, F. L. (in press). N-terminomics reveals control of Arabidopsis seed storage proteins and proteases by the Arg/N-end rule pathway. New Phytologist, https://doi.org/10.1111/nph.14909

The N-end rule pathway of targeted protein degradation is an important regulator of diverse processes in plants but detailed knowledge regarding its influence on the proteome is lacking. To investigate the impact of the Arg/N-end rule pathway on the... Read More about N-terminomics reveals control of Arabidopsis seed storage proteins and proteases by the Arg/N-end rule pathway.

The Cys-Arg/N-end rule pathway is a general sensor of abiotic stress in flowering plants (2017)
Journal Article
Vicente, J., Mendiondo, G. M., Movahedi, M., Peirats-Llobet, M., Juan, Y., Shen, Y., …Holdsworth, M. J. (2017). The Cys-Arg/N-end rule pathway is a general sensor of abiotic stress in flowering plants. Current Biology, 27(20), Article 3183-3190.e4. https://doi.org/10.1016/j.cub.2017.09.006

Abiotic stresses impact negatively on plant growth, profoundly affecting yield and quality of crops. Although much is known about plant responses, very little is understood at the molecular level about the initial sensing of environmental stress. In... Read More about The Cys-Arg/N-end rule pathway is a general sensor of abiotic stress in flowering plants.

First hints of new sensors (2017)
Journal Article
Holdsworth, M. J. (in press). First hints of new sensors. Nature Plants, 3, https://doi.org/10.1038/s41477-017-0031-7

A mechanism by which plants detect and respond to oxygen starvation has been known for some years. Three recent papers suggest that we haven’t been seeing the full picture.

Community recommendations on terminology and procedures used in flooding and low oxygen stress research (2017)
Journal Article
Sasidharan, R., Bailey-Serres, J., Ashikari, M., Atwell, B. J., Colmer, T. D., Fagerstedt, K., …Voesenek, L. A. C. J. (2017). Community recommendations on terminology and procedures used in flooding and low oxygen stress research. New Phytologist, 214(4), 1403-1407. https://doi.org/10.1111/nph.14519

Apart from playing a key role in important biochemical reactions, molecular oxygen (O2) and its by-products also have crucial signaling roles in shaping plant developmental programs and environmental responses. Even under normal conditions, sharp O2... Read More about Community recommendations on terminology and procedures used in flooding and low oxygen stress research.

Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot (2016)
Journal Article
Gravot, A., Richard, G., Lime, T., Lemarié, S., Jubault, M., Lariagon, C., …Manzanares-Dauleux, M. J. (2016). Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot. BMC Plant Biology, 16, Article 251. https://doi.org/10.1186/s12870-016-0941-y

Background The induction of alcohol fermentation in roots is a plant adaptive response to flooding stress and oxygen deprivation. Available transcriptomic data suggest that fermentation-related genes are also frequently induced in roots infected w... Read More about Hypoxia response in Arabidopsis roots infected by Plasmodiophora brassicae supports the development of clubroot.

Dormant and after-ripened Arabidopsis thaliana seeds are distinguished by early transcriptional differences in the imbibed state (2016)
Journal Article
Dekkers, B. J., Pearce, S. P., van Bolderen-Veldkamp, R., Holdsworth, M. J., & Bentsink, L. (2016). Dormant and after-ripened Arabidopsis thaliana seeds are distinguished by early transcriptional differences in the imbibed state. Frontiers in Plant Science, 7(AUG2016), Article 1323. https://doi.org/10.3389/fpls.2016.01323

Seed dormancy is a genetically controlled block preventing the germination of imbibed seeds in favorable conditions. It requires a period of dry storage (after-ripening) or certain environmental conditions to be overcome. Dormancy is an important see... Read More about Dormant and after-ripened Arabidopsis thaliana seeds are distinguished by early transcriptional differences in the imbibed state.

Oxygen Sensing Coordinates Photomorphogenesis to Facilitate Seedling Survival (2015)
Journal Article
Abbas, M., S, B., Rooney, D. J., Gibbs, D. J., Vicente Conde, J., Correia C, S., …MJ, H. (2015). Oxygen Sensing Coordinates Photomorphogenesis to Facilitate Seedling Survival. Current Biology, 25(11), 1483-1488. https://doi.org/10.1016/j.cub.2015.03.060

Successful emergence from the soil is essential for plant establishment in natural and farmed systems. It has been assumed that the absence of light in the soil is the preeminent signal perceived during early seedling development, leading to a distin... Read More about Oxygen Sensing Coordinates Photomorphogenesis to Facilitate Seedling Survival.

Group VII ethylene response factors coordinate oxygen and nitric oxide signal transduction and stress responses in plants (2015)
Journal Article
Gibbs, D. J., Vincente Conde, J., Berckhan, S., Prasad, G., Mendiondo, G. M., & Holdsworth, M. J. (2015). Group VII ethylene response factors coordinate oxygen and nitric oxide signal transduction and stress responses in plants. Plant Physiology, 169(1), https://doi.org/10.1104/pp.15.00338

The group VII ethylene response factors (ERFVIIs) are plant-specific transcription factors that have emerged as important regulators of abiotic and biotic stress responses, in particular, low-oxygen stress. A defining feature of ERFVIIs is their cons... Read More about Group VII ethylene response factors coordinate oxygen and nitric oxide signal transduction and stress responses in plants.

Multi-omics analysis identifies genes mediating the extension of cell walls in the Arabidopsis thaliana root elongation zone (2015)
Journal Article
Wilson, M. H., Holman, T. J., Sørensen, I., Cancho-Sanchez, E., Wells, D. M., Swarup, R., …Hodgman, T. C. (2015). Multi-omics analysis identifies genes mediating the extension of cell walls in the Arabidopsis thaliana root elongation zone. Frontiers in Cell and Developmental Biology, 3(FEB), Article 10. https://doi.org/10.3389/fcell.2015.00010

Plant cell wall composition is important for regulating growth rates, especially in roots. However, neither analyses of cell wall composition nor transcriptomes on their own can comprehensively reveal which genes and processes are mediating growth an... Read More about Multi-omics analysis identifies genes mediating the extension of cell walls in the Arabidopsis thaliana root elongation zone.

Enhanced waterlogging tolerance in barley by manipulation of expression of the N-end rule pathway E3 ligase PROTEOLYSIS6 (2015)
Journal Article
Mendiondo, G. M., Gibbs, D. J., Szurman-Zubrzycka, M., Korn, A., Marquez, J., Szarejko, I., …Holdsworth, M. J. (2016). Enhanced waterlogging tolerance in barley by manipulation of expression of the N-end rule pathway E3 ligase PROTEOLYSIS6. Plant Biotechnology Journal, 14(1), 40-50. https://doi.org/10.1111/pbi.12334

© 2016 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd. Increased tolerance of crops to low oxygen (hypoxia) during flooding is a key target for food security. In Arabidopsis thaliana (L.) Heynh., the N... Read More about Enhanced waterlogging tolerance in barley by manipulation of expression of the N-end rule pathway E3 ligase PROTEOLYSIS6.

Promotion of testa rupture during garden cress germination involves seed compartment-specific expression and activity of pectin methylesterases (2015)
Journal Article
Scheler, C., Weitbrecht, K., Pearce, S. P., Hampstead, A., Büttner-Mainik, A., Lee, K. J., …Leubner-Metzger, G. (2015). Promotion of testa rupture during garden cress germination involves seed compartment-specific expression and activity of pectin methylesterases. Plant Physiology, 167(1),

Pectin methylesterase (PME) controls the methylesterification status of pectins and thereby determines the biophysical properties of plant cell walls, which are important for tissue growth and weakening processes. We demonstrate here that tissue-spec... Read More about Promotion of testa rupture during garden cress germination involves seed compartment-specific expression and activity of pectin methylesterases.