All Outputs (28)

Two novel species of arctic-alpine lichen-forming fungi (Ascomycota, Megasporaceae) from the Deosai Plains, Pakistan (2024)
Journal Article
Usman, M., Dyer, P. S., Brock, M., Wade, C. M., & Khalid, A. N. (2024). Two novel species of arctic-alpine lichen-forming fungi (Ascomycota, Megasporaceae) from the Deosai Plains, Pakistan. MycoKeys, 102, 285-299. https://doi.org/10.3897/mycokeys.102.113310

Members of the lichen-forming fungal genus Oxneriaria are known to occur in cold polar and high altitudinal environments. Two new species, Oxneriaria crittendenii and O. deosaiensis, are now described from the high altitude Deosai Plains, Pakistan, b... Read More about Two novel species of arctic-alpine lichen-forming fungi (Ascomycota, Megasporaceae) from the Deosai Plains, Pakistan.

CRISPR-based tools for targeted genetic manipulation in pathogenic Sporothrix species (2023)
Journal Article
species. Microbiology Spectrum, 11(5), Article e0507822. https://doi.org/10.1128/spectrum.05078-22

is an emerging fungal pathogen frequently associated with zoonotic transmission of sporotrichosis by contaminated cats. Within 25 years, the disease has spread not only throughout Brazil but now to neighboring countries in Latin America. Thermo-dimo... Read More about CRISPR-based tools for targeted genetic manipulation in pathogenic Sporothrix species.

Powerful and Real-Time Quantification of Antifungal Efficacy against Triazole-Resistant and -Susceptible Aspergillus fumigatus Infections in Galleria mellonella by Longitudinal Bioluminescence Imaging. (2023)
Journal Article
Vanhoffelen, E., Michiels, L., Brock, M., Lagrou, K., Reséndiz-Sharpe, A., & Vande Velde, G. (2023). Powerful and Real-Time Quantification of Antifungal Efficacy against Triazole-Resistant and -Susceptible Aspergillus fumigatus Infections in Galleria mellonella by Longitudinal Bioluminescence Imaging. Microbiology Spectrum, 11(4), Article e0082523. https://doi.org/10.1128/spectrum.00825-23

Aspergillus fumigatus is an environmental mold that causes life-threatening respiratory infections in immunocompromised patients. The plateaued effectiveness of antifungal therapy and the increasing prevalence of triazole-resistant isolates have led... Read More about Powerful and Real-Time Quantification of Antifungal Efficacy against Triazole-Resistant and -Susceptible Aspergillus fumigatus Infections in Galleria mellonella by Longitudinal Bioluminescence Imaging..

Characterisation of ascocorynin biosynthesis in the purple jellydisc fungus Ascocoryne sarcoides (2022)
Journal Article
Wieder, C., Peres da Silva, R., Witts, J., Jaeger, C. M., Geib, E., & Brock, M. (2022). Characterisation of ascocorynin biosynthesis in the purple jellydisc fungus Ascocoryne sarcoides. Fungal Biology and Biotechnology, 9, Article 8. https://doi.org/10.1186/s40694-022-00138-7

Background: Non-ribosomal peptide synthetase-like (NRPS-like) enzymes are highly enriched in fungal genomes and can be discriminated into reducing and non-reducing enzymes. Non-reducing NRPS-like enzymes possess a C-terminal thioesterase domain that... Read More about Characterisation of ascocorynin biosynthesis in the purple jellydisc fungus Ascocoryne sarcoides.

Longitudinal multimodal imaging-compatible mouse model of triazole-sensitive and -resistant invasive pulmonary aspergillosis (2022)
Journal Article
Resendiz-Sharpe, A., da Silva, R. P., Geib, E., Vanderbeke, L., Seldeslachts, L., Hupko, C., …Vande Velde, G. (2022). Longitudinal multimodal imaging-compatible mouse model of triazole-sensitive and -resistant invasive pulmonary aspergillosis. Disease Models and Mechanisms, 15(3), Article dmm049165. https://doi.org/10.1242/dmm.049165

Invasive pulmonary aspergillosis (IPA) caused by the mold Aspergillus fumigatus is one of the most important life-threatening infections in immunocompromised patients. The alarming increase of isolates resistant to the first-line recommended antifung... Read More about Longitudinal multimodal imaging-compatible mouse model of triazole-sensitive and -resistant invasive pulmonary aspergillosis.

Identification of Disease-Associated Cryptococcal Proteins Reactive With Serum IgG From Cryptococcal Meningitis Patients (2021)
Journal Article
Gressler, A. E., Volke, D., Firacative, C., Schnabel, C. L., Müller, U., Krizsan, A., …Alber, G. (2021). Identification of Disease-Associated Cryptococcal Proteins Reactive With Serum IgG From Cryptococcal Meningitis Patients. Frontiers in Immunology, 12, Article 709695. https://doi.org/10.3389/fimmu.2021.709695

Cryptococcus neoformans, an opportunistic fungal pathogen ubiquitously present in the environment, causes cryptococcal meningitis (CM) mainly in immunocompromised patients, such as AIDS patients. We aimed to identify disease-associated cryptococcal p... Read More about Identification of Disease-Associated Cryptococcal Proteins Reactive With Serum IgG From Cryptococcal Meningitis Patients.

Culture degeneration reduces sex-related gene expression, alters metabolite production and reduces insect pathogenic response in cordyceps militaris (2021)
Journal Article
Wellham, P. A. D., Hafeez, A., Gregori, A., Brock, M., Kim, D. H., Chandler, D., & de Moor, C. H. (2021). Culture degeneration reduces sex-related gene expression, alters metabolite production and reduces insect pathogenic response in cordyceps militaris. Microorganisms, 9(8), Article 1559. https://doi.org/10.3390/microorganisms9081559

Cordyceps militaris is an entomopathogenic ascomycete, known primarily for infecting lepidopteran larval (caterpillars) and pupal hosts. Cordycepin, a secondary metabolite produced by this fungus has anti-inflammatory properties and other pharmacolog... Read More about Culture degeneration reduces sex-related gene expression, alters metabolite production and reduces insect pathogenic response in cordyceps militaris.

Global sexual fertility in the opportunistic pathogen aspergillus fumigatus and identification of new supermater strains (2020)
Journal Article
Swilaiman, S. S., O’Gorman, C. M., Du, W., Sugui, J. A., Del Buono, J., Brock, M., …Dyer, P. S. (2020). Global sexual fertility in the opportunistic pathogen aspergillus fumigatus and identification of new supermater strains. Journal of Fungi, 6(4), https://doi.org/10.3390/jof6040258

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. A sexual cycle in Aspergillus fumigatus was first described in 2009 with isolates from Dublin, Ireland. However, the extent to which worldwide isolates can undergo sexual reproduction has rema... Read More about Global sexual fertility in the opportunistic pathogen aspergillus fumigatus and identification of new supermater strains.

Metabolic Peculiarities of Paracoccidioides brasiliensis Dimorphism as Demonstrated by iTRAQ Labeling Proteomics (2019)
Journal Article
Araújo, D. S., Pereira, M., Portis, I. G., dos Santos Junior, A. D. C. M., Fontes, W., de Sousa, M. V., …Soares, C. M. D. A. (2019). Metabolic Peculiarities of Paracoccidioides brasiliensis Dimorphism as Demonstrated by iTRAQ Labeling Proteomics. Frontiers in Microbiology, 10, 1-12. https://doi.org/10.3389/fmicb.2019.00555

Paracoccidioidomycosis (PCM), a systemic mycosis with a high incidence in Latin America, is caused by thermodimorphic fungi of the Paracoccidioides genus. The contact with host occurs by the inhalation of conidia or mycelial propagules which once rea... Read More about Metabolic Peculiarities of Paracoccidioides brasiliensis Dimorphism as Demonstrated by iTRAQ Labeling Proteomics.

Cooperation between protein kinase A (PKA) and high osmolarity glycerol reponse (HOG) pathways is affecting cell wall carbohydrate mobilization in Aspergillus fumigatus (2018)
Journal Article
de Assis, L. J., Manfiolli, A., Mattos, E., Malavazi, I., Jacobsen, I. D., Brock, M., …Goldman, G. H. (2018). Cooperation between protein kinase A (PKA) and high osmolarity glycerol reponse (HOG) pathways is affecting cell wall carbohydrate mobilization in Aspergillus fumigatus. mBio, 9(6), Article e01952-18

Aspergillus fumigatus mitogen-activated protein kinases (MAPKs) are involved in maintaining the normal morphology of the cell wall and providing resistance against cell wall-damaging agents. Upon cell wall stress, cell wall-related sugars need to be... Read More about Cooperation between protein kinase A (PKA) and high osmolarity glycerol reponse (HOG) pathways is affecting cell wall carbohydrate mobilization in Aspergillus fumigatus.

Cross-chemistry leads to product diversity from atromentin synthetases in Aspergilli from section Nigri (2018)
Journal Article
Geib, E., Baldeweg, F., Doerfer, M., Nett, M., & Brock, M. (2019). Cross-chemistry leads to product diversity from atromentin synthetases in Aspergilli from section Nigri. Cell Chemical Biology, 26(2), 223-234.e6. https://doi.org/10.1016/j.chembiol.2018.10.021

Nonribosomal peptide synthetase (NRPS)-like enzymes catalyse the non-oxidative homodimerisation of aromatic ?-keto acids, but the exact reaction mechanism is unknown. The furanone-forming thioesterase domain of the Aspergillus terreus aspulvinone E s... Read More about Cross-chemistry leads to product diversity from atromentin synthetases in Aspergilli from section Nigri.

Monitoring of fluconazole and caspofungin activity against in vivo Candida glabrata biofilms by bioluminescence imaging (2018)
Journal Article
Persyn, A., Rogiers, O., Brock, M., Vande Velde, G., Lamkanfi, M., Jacobsen, I. D., …Kucharíková, S. (2018). Monitoring of fluconazole and caspofungin activity against in vivo Candida glabrata biofilms by bioluminescence imaging. Antimicrobial Agents and Chemotherapy, 63(2), https://doi.org/10.1128/aac.01555-18

Candida glabrata can attach to various medical implants and forms thick biofilms despite its inability to switch from-yeast-to hyphae. Current in vivo C. glabrata biofilm models only provide limited information about colonization and infection and us... Read More about Monitoring of fluconazole and caspofungin activity against in vivo Candida glabrata biofilms by bioluminescence imaging.

Hybrid in silico/in vitro target fishing to assign function to “orphan” compounds of food origin: the case of the fungal metabolite atromentin (2018)
Journal Article
Dellafiora, L., Aichinger, G., Geib, E., Sánchez-Barrionuevo, L., Brock, M., Cánovas, D., …Marko, D. (2019). Hybrid in silico/in vitro target fishing to assign function to “orphan” compounds of food origin: the case of the fungal metabolite atromentin. Food Chemistry, 270, 61-69. https://doi.org/10.1016/j.foodchem.2018.07.027

Many small molecules of food origin may effect human health but lack an adequate description of their biological activity. To fill this knowledge gap, a first-line workflow is needed to assign putative functions, rank the endpoints for testing and gu... Read More about Hybrid in silico/in vitro target fishing to assign function to “orphan” compounds of food origin: the case of the fungal metabolite atromentin.

A multimodal imaging approach enables in vivo assessment of antifungal treatment in a mouse model of invasive pulmonary aspergillosis (2018)
Journal Article
Poelmans, J., Himmelreich, U., Vanherp, L., Zhai, L., Hillen, A., Holvoet, B., …Lagrou, K. (in press). A multimodal imaging approach enables in vivo assessment of antifungal treatment in a mouse model of invasive pulmonary aspergillosis. Antimicrobial Agents and Chemotherapy, Article AAC.00240-18. https://doi.org/10.1128/AAC.00240-18

Aspergillus fumigatus causes life-threatening lung infections in immunocompromised patients. Mouse models are extensively used in research to assess the in vivo efficacy of antifungals. In recent years, there has been an increasing interest in the us... Read More about A multimodal imaging approach enables in vivo assessment of antifungal treatment in a mouse model of invasive pulmonary aspergillosis.

ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in Aspergillus niger (2017)
Journal Article
Geib, E., & Brock, M. (in press). ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in Aspergillus niger. Fungal Biology and Biotechnology, 4(1), Article e13. https://doi.org/10.1186/s40694-017-0042-1

Background: Fungi are treasure chests for yet unexplored natural products. However, exploitation of their real potential remains difficult as a significant proportion of biosynthetic gene clusters appears silent under standard laboratory conditions.... Read More about ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in Aspergillus niger.

Candida albicans Hap43 domains are required under iron starvation but not excess (2017)
Journal Article
Skrahina, V., Brock, M., Hube, B., & Brunke, S. (2017). Candida albicans Hap43 domains are required under iron starvation but not excess. Frontiers in Microbiology, 8, Article 2388. https://doi.org/10.3389/fmicb.2017.02388

Iron availability is a central factor in infections, since iron is a critical micronutrient for all living organisms. The host employs both iron limitation and toxicity strategies to control microbial growth, and successful pathogens are able to tigh... Read More about Candida albicans Hap43 domains are required under iron starvation but not excess.

Persistence within dendritic cells marks an antifungal evasion and dissemination strategy of Aspergillus terreus (2017)
Journal Article
Hsieh, S., Kurzai, O., & Brock, M. (2017). Persistence within dendritic cells marks an antifungal evasion and dissemination strategy of Aspergillus terreus. Scientific Reports, 7, https://doi.org/10.1038/s41598-017-10914-w

Aspergillus terreus is an airborne human fungal pathogen causing life-threatening invasive aspergillosis in immunocompromised patients. In contrast to Aspergillus fumigatus, A. terreus infections are associated with high dissemination rates and poor... Read More about Persistence within dendritic cells marks an antifungal evasion and dissemination strategy of Aspergillus terreus.

A highly conserved basidiomycete peptide synthetase produces a trimeric hydroxamate siderophore (2017)
Journal Article
Brandenburger, E., Gressler, M., Leonhardt, R., Lackner, G., Habel, A., Hertweck, C., …Hoffmeister, D. (in press). A highly conserved basidiomycete peptide synthetase produces a trimeric hydroxamate siderophore. Applied and Environmental Microbiology, https://doi.org/10.1128/AEM.01478-17

The model white-rot basidiomycete Ceriporiopsis (Gelatoporia) subvermispora B encodes putative natural product biosynthesis genes. Among them is the gene for the seven-domain nonribosomal peptide synthetase CsNPS2. It is a member of the as-yet unchar... Read More about A highly conserved basidiomycete peptide synthetase produces a trimeric hydroxamate siderophore.

Lipid components of bile increase the protective effect of conjugated bile salts against antifungal drugs (2017)
Journal Article
Hsieh, S., & Brock, M. (in press). Lipid components of bile increase the protective effect of conjugated bile salts against antifungal drugs. Fungal Biology, https://doi.org/10.1016/j.funbio.2017.08.002

Fungi and bacteria can persist in the human gall bladder. Previous studies have shown that bile protects Candida albicans in this cryptic host niche from antifungals, providing a reservoir for intestinal re-colonization after discontinuation of antif... Read More about Lipid components of bile increase the protective effect of conjugated bile salts against antifungal drugs.

Encapsulation of antifungals in micelles protects Candida albicans during gall-bladder infection (2017)
Journal Article
Hsieh, S., Brunke, S., & Brock, M. (2017). Encapsulation of antifungals in micelles protects Candida albicans during gall-bladder infection. Frontiers in Microbiology, 8, Article 117. https://doi.org/10.3389/fmicb.2017.00117

Candida albicans is a dimorphic fungus that colonizes human mucosal surfaces with the potential to cause life-threatening invasive candidiasis. Studies on systemic candidiasis in a murine infection model using in vivo real-time bioluminescence imagin... Read More about Encapsulation of antifungals in micelles protects Candida albicans during gall-bladder infection.