Research Repository

See what's under the surface


Bat lung epithelial cells show greater host species-specific innate resistance than MDCK cells to human and avian influenza viruses (2018)
Journal Article
Slater, T., Eckerle, I., & Chang, K. (2018). Bat lung epithelial cells show greater host species-specific innate resistance than MDCK cells to human and avian influenza viruses. Virology Journal, 15, doi:10.1186/s12985-018-0979-6. ISSN 1743-422X

Background With the recent discovery of novel H17N10 and H18N11 influenza viral RNA in bats and report on high frequency of avian H9 seroconversion in a species of free ranging bats, an important issue to address is the extent bats are susceptible... Read More

Enhanced pathogenicity and neurotropism of mouse-adapted H10N7 influenza virus are mediated by novel PB2 and NA mutations (2017)
Journal Article
Zhang, X., Xu, G., Wang, C., Jiang, M., Gao, W., Wang, M., …Pu, J. (2017). Enhanced pathogenicity and neurotropism of mouse-adapted H10N7 influenza virus are mediated by novel PB2 and NA mutations. Journal of General Virology, doi:10.1099/jgv.0.000770. ISSN 0022-1317

The H10 subtype of avian influenza viruses (AIVs) circulates globally in wild birds and poultry, and this subtype has been shown to be increasingly prevalent in China. Among the various H10 viruses, H10N7 AIVs have caused repeated mammal and human in... Read More

Prevailing PA mutation K356R in avian influenza H9N2 virus increases mammalian replication and pathogenicity (2016)
Journal Article
Xu, G., Zhang, X., Gao, W., Wang, C., Wang, J., Sun, H., …Pu, J. (in press). Prevailing PA mutation K356R in avian influenza H9N2 virus increases mammalian replication and pathogenicity. Journal of Virology, 90(18), doi:10.1128/JVI.00883-16. ISSN 0022-538X

Adaptation of the viral polymerase complex comprising PB1, PB2, and PA is necessary for efficient influenza A virus replication in new host species. We found that PA mutation K356R (PA-K356R) has become predominant since 2014 in avian H9N2 viruses in... Read More

Early apoptosis of porcine alveolar macrophages limits avian influenza virus replication and proinflammatory dysregulation (2015)
Journal Article
influenza virus replication and proinflammatory dysregulation. Scientific Reports, 5(17999), doi:10.1038/srep17999. ISSN 2045-2322

Pigs are evidently more resistant to avian than swine influenza A viruses, mediated in part through frontline epithelial cells and alveolar macrophages (AM). Although porcine AM (PAM) are crucial in influenza virus control, their mode of control is u... Read More

Extended 2D myotube culture recapitulates postnatal fibre type plasticity (2015)
Journal Article
Sebastian, S., Goulding, L., Kuchipudi, S. V., & Chang, K. (in press). Extended 2D myotube culture recapitulates postnatal fibre type plasticity. BMC Cell Biology, 16(1), doi:10.1186/s12860-015-0069-1. ISSN 1471-2121

Background: The traditional problems of performing skeletal muscle cell cultures derived from mammalian or avian species are limited myotube differentiation, and transient myotube persistence which greatly restricts the ability of myotubes to undergo... Read More

PA-X is a virulence factor in avian H9N2 influenza virus (2015)
Journal Article
Gao, H., Liu, J., Kong, W., Sun, H., Pu, J., Chang, K., …Wang, J. (2015). PA-X is a virulence factor in avian H9N2 influenza virus. Journal of General Virology, 96(9), doi:10.1099/jgv.0.000232. ISSN 0022-1317

H9N2 influenza viruses have been circulating worldwide in multiple avian species, and regularly infect pigs and humans. Recently, a novel protein, PA-X, produced from the PA gene by ribosomal frameshifting, was demonstrated to be an antivirulence fac... Read More

Twenty amino acids at the C-terminus of PA-X are associated with increased influenza A virus replication and pathogenicity (2015)
Journal Article
Gao, H., Sun, Y., Liu, X., Sun, H., Hu, J., Wang, J., …He, Q. (2015). Twenty amino acids at the C-terminus of PA-X are associated with increased influenza A virus replication and pathogenicity. Journal of General Virology, 96(8), doi:10.1099/vir.0.000143. ISSN 0022-1317

The PA-X protein, arising from ribosomal frameshift during PA translation, was recently discovered in influenza A virus (IAV). The C-terminal domain ‘X’ of PA-X proteins in IAVs can be classified as full-length (61 aa) or truncated (41 aa). In the ma... Read More

Investigation into the animal species contents of popular wet pet foods (2015)
Journal Article
Maine, I. R., Atterbury, R. J., & Chang, K. (2015). Investigation into the animal species contents of popular wet pet foods. Acta Veterinaria Scandinavica, 57(1), doi:10.1186/s13028-015-0097-z. ISSN 1751-0147

Background: The use of the generic term “meat and animal derivatives” in declared ingredient lists of pet foods in the European Union is virtually universal. In the wake of the 2013 “horse meat scandal” in the human food chain, we examined the presen... Read More

DNA microarray global gene expression analysis of influenza virus-infected chicken and duck cells (2015)
Journal Article
Kuchipudi, S., Chang, K., & Dunham, S. (2015). DNA microarray global gene expression analysis of influenza virus-infected chicken and duck cells. Genomics Data, 4, 60-64. doi:10.1016/j.gdata.2015.03.004

The data described in this article pertain to the article by Kuchipudi et al. (2014) titled “Highly Pathogenic Avian Influenza Virus Infection in Chickens But Not Ducks Is Associated with Elevated Host Immune and Pro-inflammatory Responses” [1]. Whil... Read More

The contribution of PA-X to the virulence of pandemic 2009 H1N1 and highly pathogenic H5N1 avian influenza viruses (2015)
Journal Article
Gao, H., Sun, Y., Hu, J., Qi, L., Wang, J., Xiong, X., …Liu, J. (in press). The contribution of PA-X to the virulence of pandemic 2009 H1N1 and highly pathogenic H5N1 avian influenza viruses. Scientific Reports, 5(1), doi:10.1038/srep08262. ISSN 2045-2322

PA-X is a novel protein encoded by PA mRNA and is found to decrease the pathogenicity of pandemic 1918 H1N1 virus in mice. However, the importance of PA-X proteins in current epidemiologically important influenza A virus strains is not known. In this... Read More

Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection (2014)
Journal Article
Baquero-Perez, B., Kuchipudi, S. V., Ho, J., Sebastian, S., Puranik, A., Howard, W., …Chang, K. (2015). Chicken and duck myotubes are highly susceptible and permissive to influenza virus infection. Journal of Virology, 89(5), doi:10.1128/JVI.03421-14. ISSN 0022-538X

Skeletal muscle, at 30 to 40% of body mass, is the most abundant soft tissue in the body. Besides its primary function in movement and posture, skeletal muscle is a significant innate immune organ with the capacity to produce cytokines and chemokines... Read More

High basal expression of interferon-stimulated genes in human bronchial epithelial (BEAS-2B) cells contributes to influenza A virus resistance (2014)
Journal Article
Seng, L., Daly, J. M., Chang, K., & Kuchipudi, S. V. (2014). High basal expression of interferon-stimulated genes in human bronchial epithelial (BEAS-2B) cells contributes to influenza A virus resistance. PLoS ONE, 9, doi:10.1371/journal.pone.0109023. ISSN 1932-6203

Respiratory epithelial cells play a key role in influenza A virus (IAV) pathogenesis and host innate response. Transformed human respiratory cell lines are widely used in the study of IAV−host interactions due to their relative convenience, and inher... Read More