All Outputs (8)

Triblock copolymer micelles enhance solubility, permeability and activity of a quorum sensing inhibitor against Pseudomonas aeruginosa biofilms (2024)
Journal Article
Kasza, K., Soukarieh, F., Romero, M., Hardie, K. R., Gurnani, P., Cámara, M., & Alexander, C. (2024). Triblock copolymer micelles enhance solubility, permeability and activity of a quorum sensing inhibitor against Pseudomonas aeruginosa biofilms. RSC Applied Polymers, https://doi.org/10.1039/D3LP00208J

Antimicrobial resistance is a threat to public health for which new treatments are urgently required. The capability of bacteria to form biofilms is of particular concern as it enables high bacterial tolerance to conventional therapies by reducing dr... Read More about Triblock copolymer micelles enhance solubility, permeability and activity of a quorum sensing inhibitor against Pseudomonas aeruginosa biofilms.

Ciprofloxacin poly(β-amino ester) conjugates enhance antibiofilm activity and slow the development of resistance (2024)
Journal Article
Kasza, K., Richards, B., Jones, S., Romero, M., Robertson, S. N., Hardie, K. R., …Alexander, C. (2024). Ciprofloxacin poly(β-amino ester) conjugates enhance antibiofilm activity and slow the development of resistance. ACS Applied Materials and Interfaces, 16(5), 5412-5425. https://doi.org/10.1021/acsami.3c14357

To tackle the emerging antibiotic resistance crisis, novel antimicrobial approaches are urgently needed. Bacterial biofilms are a particular concern in this context as they are responsible for over 80% of bacterial infections and are inherently more... Read More about Ciprofloxacin poly(β-amino ester) conjugates enhance antibiofilm activity and slow the development of resistance.

Growth rate and nutrient limitation as key drivers of extracellular quorum sensing signal molecule accumulation in Pseudomonas aeruginosa (2023)
Journal Article
Dubern, J., Halliday, N., Cámara, M., Winzer, K., Barrett, D. A., Hardie, K. R., & Williams, P. (2023). Growth rate and nutrient limitation as key drivers of extracellular quorum sensing signal molecule accumulation in Pseudomonas aeruginosa. Microbiology, 169(4), Article 001316. https://doi.org/10.1099/mic.0.001316

In Pseudomonas aeruginosa, quorum sensing (QS) depends on an interconnected regulatory hierarchy involving the Las, Rhl and Pqs systems, which are collectively responsible for the co-ordinated synthesis of a diverse repertoire of N-acylhomoserine lac... Read More about Growth rate and nutrient limitation as key drivers of extracellular quorum sensing signal molecule accumulation in Pseudomonas aeruginosa.

The biocide triclosan induces (p)ppGpp dependent antibiotic tolerance and alters SarA dependent biofilm structures in Staphylococcus aureus (2023)
Working Paper
Walsh, D., Salzer, A., Wolz, C., Aylott, J., & Hardie, K. R. The biocide triclosan induces (p)ppGpp dependent antibiotic tolerance and alters SarA dependent biofilm structures in Staphylococcus aureus

The biocide triclosan is used extensively in both household and hospital settings. The chronic exposure to the biocide occurring in individuals that use triclosan-containing products results in low levels of triclosan present in the human body that h... Read More about The biocide triclosan induces (p)ppGpp dependent antibiotic tolerance and alters SarA dependent biofilm structures in Staphylococcus aureus.

Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation (2021)
Journal Article
Hollmann, B., Perkins, M., Chauhan, V. M., Aylott, J. W., & Hardie, K. R. (2021). Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation. npj Biofilms and Microbiomes, 7(1), Article 50. https://doi.org/10.1038/s41522-021-00221-8

Understanding the dynamic environmental microniches of biofilms will permit us to detect, manage and exploit these communities. The components and architecture of biofilms have been interrogated in depth; however, little is known about the environmen... Read More about Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation.

A Simple Polymicrobial Biofilm Keratinocyte Colonization Model for Exploring Interactions Between Commensals, Pathogens and Antimicrobials (2020)
Journal Article
Jordana-Lluch, E., Garcia, V., Kingdon, A. D. H., Singh, N., Alexander, C., Williams, P., & Hardie, K. R. (2020). A Simple Polymicrobial Biofilm Keratinocyte Colonization Model for Exploring Interactions Between Commensals, Pathogens and Antimicrobials. Frontiers in Microbiology, 11, Article 291. https://doi.org/10.3389/fmicb.2020.00291

© 2020 Jordana-Lluch, Garcia, Kingdon, Singh, Alexander, Williams and Hardie. Skin offers protection against external insults, with the skin microbiota playing a crucial defensive role against pathogens that gain access when the skin barrier is breac... Read More about A Simple Polymicrobial Biofilm Keratinocyte Colonization Model for Exploring Interactions Between Commensals, Pathogens and Antimicrobials.

Dual Bioresponsive Antibiotic and Quorum Sensing Inhibitor Combination Nanoparticles for Treatment of Pseudomonas aeruginosa Biofilms In Vitro and Ex Vivo (2019)
Journal Article
Singh, N., Romero, M., Travanut, A., Monteiro, P., Jordana-Lluch, E., Hardie, K. R., …Alexander, C. (2019). Dual Bioresponsive Antibiotic and Quorum Sensing Inhibitor Combination Nanoparticles for Treatment of Pseudomonas aeruginosa Biofilms In Vitro and Ex Vivo. Biomaterials Science, 10(7), 4099-4111. https://doi.org/10.1039/C9BM00773C

Many debilitating infections result from persistent microbial biofilms that do not respond to conventional antibiotic regimens. A potential method to treat such chronic infections is to combine agents which interfere with bacterial biofilm developmen... Read More about Dual Bioresponsive Antibiotic and Quorum Sensing Inhibitor Combination Nanoparticles for Treatment of Pseudomonas aeruginosa Biofilms In Vitro and Ex Vivo.

The fitness burden imposed by synthesising quorum sensing signals (2016)
Journal Article
Ruparell, A., Dubern, J., Ortori, C. A., Harrison, F., Halliday, N., Emtage, A., …Hardie, K. R. (2016). The fitness burden imposed by synthesising quorum sensing signals. Scientific Reports, 6, Article 33101. https://doi.org/10.1038/srep33101

It is now well established that bacterial populations utilize cell-to-cell signaling (quorum-sensing, QS) to control the production of public goods and other co-operative behaviours. Evolutionary theory predicts that both the cost of signal productio... Read More about The fitness burden imposed by synthesising quorum sensing signals.