Skip to main content

Research Repository

Advanced Search

Structure-based design of hepatitis C virus E2 glycoprotein improves serum binding and cross-neutralization (2020)
Journal Article
Pierce, B. G., Keck, Z., Wang, R., Lau, P., Garagusi, K., Elkholy, K., …Foung, S. K. (2020). Structure-based design of hepatitis C virus E2 glycoprotein improves serum binding and cross-neutralization. Journal of Virology, https://doi.org/10.1128/jvi.00704-20

An effective vaccine for hepatitis C virus (HCV) is a major unmet need, and it requires an antigen that elicits immune responses to key conserved epitopes. Based on structures of antibodies targeting HCV envelope glycoprotein E2, we designed immunoge... Read More about Structure-based design of hepatitis C virus E2 glycoprotein improves serum binding and cross-neutralization.

Adjuvant formulated virus-like particles expressing native-like forms of the Lassa virus envelope surface glycoprotein are immunogenic and induce antibodies with broadly neutralizing activity (2020)
Journal Article
Müller, H., Fehling, S. K., Dorna, J., Urbanowicz, R. A., Oestereich, L., Krebs, Y., …Strecker, T. (2020). Adjuvant formulated virus-like particles expressing native-like forms of the Lassa virus envelope surface glycoprotein are immunogenic and induce antibodies with broadly neutralizing activity. npj Vaccines, 5, https://doi.org/10.1038/s41541-020-00219-x

Lassa mammarenavirus (LASV) is a rodent-borne arenavirus endemic to several West African countries. It is the causative agent of human Lassa fever, an acute viral hemorrhagic fever disease. To date, no therapeutics or vaccines against LASV have obtai... Read More about Adjuvant formulated virus-like particles expressing native-like forms of the Lassa virus envelope surface glycoprotein are immunogenic and induce antibodies with broadly neutralizing activity.

A next generation vaccine against human rabies based on a single dose of a chimpanzee adenovirus vector serotype C (2020)
Journal Article
Napolitano, F., Merone, R., Abbate, A., Ammendola, V., Horncastle, E., Lanzaro, F., …Vitelli, A. (2020). A next generation vaccine against human rabies based on a single dose of a chimpanzee adenovirus vector serotype C. PLoS Neglected Tropical Diseases, 14(7), https://doi.org/10.1371/journal.pntd.0008459

Rabies, caused by RNA viruses in the Genus Lyssavirus, is the most fatal of all infectious diseases. This neglected zoonosis remains a major public health problem in developing countries, causing the death of an estimated 25,000-159,000 people each y... Read More about A next generation vaccine against human rabies based on a single dose of a chimpanzee adenovirus vector serotype C.

Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species (2020)
Journal Article
Chappell, J. G., Tsoleridis, T., Onianwa, O., Drake, G., Ashpole, I., Dobbs, P., …McClure, C. P. (2020). Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species. Viruses, 12(4), https://doi.org/10.3390/v12040454

©2020 by the authors. Licensee MDPI, Basel, Switzerland. Orthohantaviruses are globally distributed viruses, associated with rodents and other small mammals. However, data on the circulation of orthohantaviruses within the UK, particularly the UK-end... Read More about Retrieval of the Complete Coding Sequence of the UK-Endemic Tatenale Orthohantavirus Reveals Extensive Strain Variation and Supports Its Classification as a Novel Species.

Hepatitis C virus vaccine: Challenges and prospects (2020)
Journal Article
Duncan, J. D., Urbanowicz, R. A., Tarr, A. W., & Ball, J. K. (2020). Hepatitis C virus vaccine: Challenges and prospects. Vaccines, 8(1), https://doi.org/10.3390/vaccines8010090

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The hepatitis C virus (HCV) causes both acute and chronic infection and continues to be a global problem despite advances in antiviral therapeutics. Current treatments fail to prevent reinfect... Read More about Hepatitis C virus vaccine: Challenges and prospects.

Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits (2019)
Journal Article
Tsoleridis, T., Chappell, J. G., Monchatre-Leroy, E., Umhang, G., Shi, M., Bennett, M., …Ball, J. K. (2019). Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits. Viruses, 12(1), https://doi.org/10.3390/v12010047

The advent of unbiased metagenomic virus discovery has revolutionized studies of virus biodiversity and evolution. Despite this, our knowledge of the virosphere, including in mammalian species, remains limited. We used unbiased metagenomic sequencing... Read More about Discovery and Prevalence of Divergent RNA Viruses in European Field Voles and Rabbits.

IFITMs mediate viral evasion in acute and chronic hepatitis C virus infection (2019)
Journal Article
Wrensch, F., Ligat, G., Heydmann, L., Schuster, C., Zeisel, M. B., Pessaux, P., …Baumert, T. F. (2019). IFITMs mediate viral evasion in acute and chronic hepatitis C virus infection. Hepatology, 70(5), 1506-1520. https://doi.org/10.1002/hep.30699

While adaptive immune responses against hepatitis C virus (HCV) infection have been studied in great detail, the role of innate immunity in protection against HCV infection and immune evasion is only partially understood. Interferon‐induced transmemb... Read More about IFITMs mediate viral evasion in acute and chronic hepatitis C virus infection.

Cross-genotype AR3-specific neutralizing antibodies confer long-term protection in injecting drug users after HCV clearance (2019)
Journal Article
Merat, S. J., Bru, C., van de Berg, D., Molenkamp, R., Tarr, A. W., Koekkoek, S., …Schinkel, J. (2019). Cross-genotype AR3-specific neutralizing antibodies confer long-term protection in injecting drug users after HCV clearance. Journal of Hepatology, 71(1), 14-24. https://doi.org/10.1016/j.jhep.2019.02.013

Background & Aims Understanding immune protection against hepatitis C virus (HCV) infection is necessary for designing an effective vaccine. A number of broadly-reactive, neutralizing antibodies have been isolated from B cells of HCV-infected subjec... Read More about Cross-genotype AR3-specific neutralizing antibodies confer long-term protection in injecting drug users after HCV clearance.

Expression of human ficolin-2 in hepatocytes confers resistance to infection by diverse hepatotropic viruses (2019)
Journal Article
Irving, W. L., Jalal, P. J., Urbanowicz, R. A., Horncastle, E., Pathak, M., Goddard, C., …Tarr, A. W. (2019). Expression of human ficolin-2 in hepatocytes confers resistance to infection by diverse hepatotropic viruses. Journal of Medical Microbiology, 68(4), 642-648. https://doi.org/10.1099/jmm.0.000935

The liver-expressed pattern recognition receptors (PRRs) mannose binding lectin (MBL), ficolin-2 and ficolin-3 contribute to the innate immune response by activating complement. Binding of soluble ficolin-2 to viral pathogens can directly neutralize... Read More about Expression of human ficolin-2 in hepatocytes confers resistance to infection by diverse hepatotropic viruses.

Shared common ancestry of rodent alphacoronaviruses sampled globally (2019)
Journal Article
Tsoleridis, T., Chappell, J. G., Onianwa, O., Marston, D. A., Fooks, A. R., Monchatre‐leroy, E., …K 7 Ball, J. (2019). Shared common ancestry of rodent alphacoronaviruses sampled globally. Viruses, 11(2), https://doi.org/10.3390/v11020125

The recent discovery of novel alphacoronaviruses (alpha-CoVs) in European and Asian rodents revealed that rodent coronaviruses (CoVs) sampled worldwide formed a discrete phylogenetic group within this genus. To determine the evolutionary history of r... Read More about Shared common ancestry of rodent alphacoronaviruses sampled globally.

Antigenicity and immunogenicity of differentially glycosylated HCV E2 envelope proteins expressed in mammalian and insect cells (2019)
Journal Article
Urbanowicz, R. A., Wang, R., Schiel, J. E., Keck, Z., Kerzic, M. C., Lau, P., …Fuerst, T. R. (2019). Antigenicity and immunogenicity of differentially glycosylated HCV E2 envelope proteins expressed in mammalian and insect cells. Journal of Virology, 93(7), https://doi.org/10.1128/jvi.01403-18

Development of a prophylactic vaccine for hepatitis C virus (HCV) remains a global health challenge. Cumulative evidence supports the importance of antibodies targeting the HCV E2 envelope glycoprotein to facilitate viral clearance. However, a signif... Read More about Antigenicity and immunogenicity of differentially glycosylated HCV E2 envelope proteins expressed in mammalian and insect cells.

Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp) (2018)
Book Chapter
Bailey, J. R., Urbanowicz, R. A., Ball, J. K., Law, M., & Foung, S. K. H. (2019). Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp). In M. Law (Ed.), Hepatitis C Virus Protocols, 441-450. Springer. doi:10.1007/978-1-4939-8976-8_30

Hepatitis C virus (HCV) pseudoparticles (HCVpp) are generated by cotransfection of HCV envelope (E1 and E2) genes along with a retroviral packaging/reporter construct into HEK293T cells. Enveloped particles bearing HCV E1E2 proteins on their surface... Read More about Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp).

Cloning and analysis of authentic patient-derived HCV E1/E2 glycoproteins (2018)
Book Chapter
Urbanowicz, R. A., Ball, J. K., & Tarr, A. W. (2019). Cloning and analysis of authentic patient-derived HCV E1/E2 glycoproteins. In M. Law (Ed.), Hepatitis C Virus Protocols, 275-294. Springer Publishing Company. doi:10.1007/978-1-4939-8976-8_19

Experimental characterization of the properties of authentic viruses circulating in infected individuals presents a problem when investigating RNA viruses with error-prone polymerases. The hepatitis C virus provides an extreme example of RNA virus ge... Read More about Cloning and analysis of authentic patient-derived HCV E1/E2 glycoproteins.

InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones (2018)
Book Chapter
King, B., Urbanowicz, R., Tarr, A. W., Ball, J. K., & McClure, C. P. (2019). InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones. In M. Law (Ed.), Hepatitis C Virus Protocols, 93-104. Springer. doi:10.1007/978-1-4939-8976-8_6

This chapter describes how to generate chimeric molecular cassettes that are ready to receive PCR-amplified E1/E2 genes using new DNA cloning technology. The method is divided into three sections: (1) generation of a ΔCore-NS2 cassette based upon the... Read More about InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones.

Immunization with a synthetic consensus hepatitis C virus E2 glycoprotein ectodomain elicits virus-neutralizing antibodies (2018)
Journal Article
Tarr, A. W., Backx, M., Hamed, M. R., Urbanowicz, R. A., McClure, C. P., Brown, R. J., & Ball, J. K. (2018). Immunization with a synthetic consensus hepatitis C virus E2 glycoprotein ectodomain elicits virus-neutralizing antibodies. Antiviral Research, 160, 25-37. doi:10.1016/j.antiviral.2018.09.005

Global eradication of hepatitis C virus (HCV) infection will require an efficacious vaccine capable of eliciting protective immunity against genetically diverse HCV strains. Natural spontaneous resolution of HCV infection is associated with productio... Read More about Immunization with a synthetic consensus hepatitis C virus E2 glycoprotein ectodomain elicits virus-neutralizing antibodies.

Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1 (2017)
Journal Article
Mesalam, A. A., Desombere, I., Farhoudi, A., Van Houtte, F., Verhoye, L., Ball, J., …Meuleman, P. (2018). Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1. Virology, 514, https://doi.org/10.1016/j.virol.2017.10.019

Monoclonal antibodies (mAbs) targeting the hepatitis C virus (HCV) envelope have been raised mainly against envelope protein 2 (E2), while the antigenic epitopes of envelope protein 1 (E1) are not fully identified. Here we describe the detailed chara... Read More about Development and characterization of a human monoclonal antibody targeting the N-terminal region of hepatitis C virus envelope glycoprotein E1.

A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo (2017)
Journal Article
Desombere, I., Mesalam, A. A., Urbanowicz, R. A., Van Houtte, F., Verhoye, L., Keck, Z., …Meuleman, P. (2017). A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo. Antiviral Research, 148, https://doi.org/10.1016/j.antiviral.2017.10.015

Infections with hepatitis C virus (HCV) represent a worldwide health burden and a prophylactic vaccine is still not available. Liver transplantation (LT) is often the only option for patients with HCV-induced end-stage liver disease. However, immedia... Read More about A novel neutralizing human monoclonal antibody broadly abrogates hepatitis C virus infection in vitro and in vivo.

Human Adaptation of Ebola Virus during the West African Outbreak (2016)
Journal Article
Urbanowicz, R. A., McClure, C. P., Sakuntabhai, A., Sall, A. A., Kobinger, G., Müller, M. A., …Ball, J. K. (2016). Human Adaptation of Ebola Virus during the West African Outbreak. Cell, 167(4), 1079-1087.e5. https://doi.org/10.1016/j.cell.2016.10.013

The 2013–2016 outbreak of Ebola virus (EBOV) in West Africa was the largest recorded. It began following the cross-species transmission of EBOV from an animal reservoir, most likely bats, into humans, with phylogenetic analysis revealing the cocircul... Read More about Human Adaptation of Ebola Virus during the West African Outbreak.

Broad neutralization of hepatitis C virus-resistant variants by Civacir hepatitis C immunoglobulin (2016)
Journal Article
Tawar, R. G., Heydmann, L., Bach, C., Schüttrumpf, J., Chavan, S., King, B. J., …Baumert, T. (2016). Broad neutralization of hepatitis C virus-resistant variants by Civacir hepatitis C immunoglobulin. Hepatology, 64(5), 1495-1506. https://doi.org/10.1002/hep.28767

Hepatitis C virus (HCV)-induced end-stage liver disease is the major indication for liver transplantation (LT). However, reinfection of the liver graft is still common, especially in patients with detectable viral load at the time of LT. Limited data... Read More about Broad neutralization of hepatitis C virus-resistant variants by Civacir hepatitis C immunoglobulin.


;