Research Repository

See what's under the surface


Expression of human ficolin-2 in hepatocytes confers resistance to infection by diverse hepatotropic viruses (2019)
Journal Article
Jalal, P. J., Urbanowicz, R. A., Horncastle, E., Pathak, M., Goddard, C., Saeed, A., …Tarr, A. W. (2019). Expression of human ficolin-2 in hepatocytes confers resistance to infection by diverse hepatotropic viruses. Journal of Medical Microbiology, doi:10.1099/jmm.0.000935

The liver-expressed pattern recognition receptors (PRRs) mannose binding lectin (MBL), ficolin-2 and ficolin-3 contribute to the innate immune response by activating complement. Binding of soluble ficolin-2 to viral pathogens can directly neutralize... Read More

Shared common ancestry of rodent alphacoronaviruses sampled globally (2019)
Journal Article
Tsoleridis, T., Chappell, J. G., Onianwa, O., Marston, D. A., Fooks, A. R., Monchatre‐leroy, E., …K 7 Ball, J. (2019). Shared common ancestry of rodent alphacoronaviruses sampled globally. Viruses, 11(2), doi:10.3390/v11020125

The recent discovery of novel alphacoronaviruses (alpha-CoVs) in European and Asian rodents revealed that rodent coronaviruses (CoVs) sampled worldwide formed a discrete phylogenetic group within this genus. To determine the evolutionary history of r... Read More

Antigenicity and immunogenicity of differentially glycosylated HCV E2 envelope proteins expressed in mammalian and insect cells (2019)
Journal Article
Urbanowicz, R. A., Wang, R., Schiel, J. E., Keck, Z., Kerzic, M. C., Lau, P., …Fuerst, T. R. (2019). Antigenicity and immunogenicity of differentially glycosylated HCV E2 envelope proteins expressed in mammalian and insect cells. Journal of Virology, doi:10.1128/jvi.01403-18

Development of a prophylactic vaccine for hepatitis C virus (HCV) remains a global health challenge. Cumulative evidence supports the importance of antibodies targeting the HCV E2 envelope glycoprotein to facilitate viral clearance. However, a signif... Read More

InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones (2018)
Book Chapter
King, B., Urbanowicz, R., Tarr, A. W., Ball, J. K., & McClure, C. P. (2019). InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones. In M. Law (Ed.), Hepatitis C Virus Protocols, 93-104. Springer. doi:10.1007/978-1-4939-8976-8_6

This chapter describes how to generate chimeric molecular cassettes that are ready to receive PCR-amplified E1/E2 genes using new DNA cloning technology. The method is divided into three sections: (1) generation of a ΔCore-NS2 cassette based upon the... Read More

Cloning and analysis of authentic patient-derived HCV E1/E2 glycoproteins (2018)
Book Chapter
Urbanowicz, R. A., Ball, J. K., & Tarr, A. W. (2019). Cloning and analysis of authentic patient-derived HCV E1/E2 glycoproteins. In M. Law (Ed.), Hepatitis C Virus Protocols, 275-294. Springer Publishing Company. doi:10.1007/978-1-4939-8976-8_19

Experimental characterization of the properties of authentic viruses circulating in infected individuals presents a problem when investigating RNA viruses with error-prone polymerases. The hepatitis C virus provides an extreme example of RNA virus ge... Read More

Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp) (2018)
Book Chapter
Bailey, J. R., Urbanowicz, R. A., Ball, J. K., Law, M., & Foung, S. K. H. (2019). Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp). In M. Law (Ed.), Hepatitis C Virus Protocols, 441-450. Springer. doi:10.1007/978-1-4939-8976-8_30

Hepatitis C virus (HCV) pseudoparticles (HCVpp) are generated by cotransfection of HCV envelope (E1 and E2) genes along with a retroviral packaging/reporter construct into HEK293T cells. Enveloped particles bearing HCV E1E2 proteins on their surface... Read More

Human adaptation of Ebola virus during the West African outbreak (2016)
Journal Article
Urbanowicz, R. A., McClure, C. P., Sakuntabhai, A., Sall, A. A., Kobinger, G., Müller, M. A., …Ball, J. K. (in press). Human adaptation of Ebola virus during the West African outbreak. Cell, 167(4), doi:10.1016/j.cell.2016.10.013. ISSN 0092-8674

The 2013–2016 outbreak of Ebola virus (EBOV) in West Africa was the largest recorded. It began following the cross-species transmission of EBOV from an animal reservoir, most likely bats, into humans, with phylogenetic analysis revealing the cocircul... Read More

Broad neutralization of hepatitis C virus-resistant variants by Civacir hepatitis C immunoglobulin (2016)
Journal Article
Tawar, R. G., Heydmann, L., Bach, C., Schüttrumpf, J., Chavan, S., King, B. J., …Baumert, T. (2016). Broad neutralization of hepatitis C virus-resistant variants by Civacir hepatitis C immunoglobulin. Hepatology, 64(5), doi:10.1002/hep.28767. ISSN 0270-9139

Hepatitis C virus (HCV)-induced end-stage liver disease is the major indication for liver transplantation (LT). However, reinfection of the liver graft is still common, especially in patients with detectable viral load at the time of LT. Limited data... Read More

Novel functional hepatitis C virus glycoprotein isolates identified using an optimised viral pseudotype entry assay (2016)
Journal Article
Urbanowicz, R. A., King, B., Ball, J. K., McClure, C. P., Tarr, A. W., & Mason, C. P. (in press). Novel functional hepatitis C virus glycoprotein isolates identified using an optimised viral pseudotype entry assay. Journal of General Virology, 97(9), doi:10.1099/jgv.0.000537. ISSN 0022-1317

Retrovirus pseudotypes are a highly tractable model used to study the entry pathways of enveloped viruses. This model has been extensively applied to the study of the hepatitis C virus (HCV) entry pathway, pre-clinical screening of antiviral antibodi... Read More

Flexible and rapid construction of viral chimeras applied to Hepatitis C Virus (2016)
Journal Article
McClure, P. C., Urbanowicz, R. A., King, B. J., Cano-Crespo, S., Tarr, A. W., & Ball, J. K. (in press). Flexible and rapid construction of viral chimeras applied to Hepatitis C Virus. Journal of General Virology, 97(7), doi:10.1099/jgv.0.000530. ISSN 0022-1317

A novel and broadly applicable strategy combining site directed mutagenesis and DNA assembly for constructing seamless viral chimeras is described using Hepatitis C Virus as an exemplar. Full-length HCV genomic cloning cassettes, which contained flex... Read More

Novel human anti-claudin 1 mAbs inhibit hepatitis C virus infection and may synergize with anti-SRB1 mAb (2016)
Journal Article
Paciello, R., Urbanowicz, R. A., Riccio, G., Sasso, E., McClure, C. P., Zambrano, N., …De Lorenzo, C. (in press). Novel human anti-claudin 1 mAbs inhibit hepatitis C virus infection and may synergize with anti-SRB1 mAb. Journal of General Virology, 97(1), doi:10.1099/jgv.0.000330. ISSN 0022-1317

Hepatitis C virus (HCV) is a major cause of chronic hepatitis and liver carcinoma and new therapies based on novel targets are needed. The tight junction protein claudin 1 (CLDN-1) is essential for HCV cell entry and spread, and anti-CLDN-1 rat and m... Read More