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Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp) (2018)
Book Chapter
Bailey, J. R., Urbanowicz, R. A., Ball, J. K., Law, M., & Foung, S. K. H. (2019). Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp). In M. Law (Ed.), Hepatitis C Virus Protocols, 441-450. Springer. doi:10.1007/978-1-4939-8976-8_30

Hepatitis C virus (HCV) pseudoparticles (HCVpp) are generated by cotransfection of HCV envelope (E1 and E2) genes along with a retroviral packaging/reporter construct into HEK293T cells. Enveloped particles bearing HCV E1E2 proteins on their surface... Read More about Standardized method for the study of antibody neutralization of HCV pseudoparticles (HCVpp).

Cloning and analysis of authentic patient-derived HCV E1/E2 glycoproteins (2018)
Book Chapter
Urbanowicz, R. A., Ball, J. K., & Tarr, A. W. (2019). Cloning and analysis of authentic patient-derived HCV E1/E2 glycoproteins. In M. Law (Ed.), Hepatitis C Virus Protocols, 275-294. Springer Publishing Company. doi:10.1007/978-1-4939-8976-8_19

Experimental characterization of the properties of authentic viruses circulating in infected individuals presents a problem when investigating RNA viruses with error-prone polymerases. The hepatitis C virus provides an extreme example of RNA virus ge... Read More about Cloning and analysis of authentic patient-derived HCV E1/E2 glycoproteins.

InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones (2018)
Book Chapter
King, B., Urbanowicz, R., Tarr, A. W., Ball, J. K., & McClure, C. P. (2019). InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones. In M. Law (Ed.), Hepatitis C Virus Protocols, 93-104. Springer. doi:10.1007/978-1-4939-8976-8_6

This chapter describes how to generate chimeric molecular cassettes that are ready to receive PCR-amplified E1/E2 genes using new DNA cloning technology. The method is divided into three sections: (1) generation of a ΔCore-NS2 cassette based upon the... Read More about InFusion cloning for the generation of biologically relevant HCV chimeric molecular clones.

Immunization with a synthetic consensus hepatitis C virus E2 glycoprotein ectodomain elicits virus-neutralizing antibodies (2018)
Journal Article
Tarr, A. W., Backx, M., Hamed, M. R., Urbanowicz, R. A., McClure, C. P., Brown, R. J., & Ball, J. K. (2018). Immunization with a synthetic consensus hepatitis C virus E2 glycoprotein ectodomain elicits virus-neutralizing antibodies. Antiviral Research, 160, 25-37. doi:10.1016/j.antiviral.2018.09.005

Global eradication of hepatitis C virus (HCV) infection will require an efficacious vaccine capable of eliciting protective immunity against genetically diverse HCV strains. Natural spontaneous resolution of HCV infection is associated with productio... Read More about Immunization with a synthetic consensus hepatitis C virus E2 glycoprotein ectodomain elicits virus-neutralizing antibodies.


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