All Outputs (10)

A Comparison of Primary Human Hepatocytes and Hepatoma Cell Lines to Model the Effects of Fatty Acids, Fructose and Glucose on Liver Cell Lipid Accumulation (2022)
Journal Article
Huggett, Z. J., Smith, A., De Vivo, N., Gomez, D., Jethwa, P., Brameld, J. M., …Salter, A. M. (2022). A Comparison of Primary Human Hepatocytes and Hepatoma Cell Lines to Model the Effects of Fatty Acids, Fructose and Glucose on Liver Cell Lipid Accumulation. Nutrients, 15(1), Article 40. https://doi.org/10.3390/nu15010040

Non-alcoholic fatty liver disease (NAFLD) begins with lipid accumulation within hepatocytes, but the relative contributions of different macronutrients is still unclear. We investigated the impact of fatty acids, glucose and fructose on lipid accumul... Read More about A Comparison of Primary Human Hepatocytes and Hepatoma Cell Lines to Model the Effects of Fatty Acids, Fructose and Glucose on Liver Cell Lipid Accumulation.

Effect of AAV-mediated overexpression of ATF5 and downstream targets of an integrated stress response in murine skeletal muscle (2021)
Journal Article
Brearley-Sholto, M. C., Loczenski-Brown, D. M., Jones, S., Daniel, Z. C., Ebling, F. J., Parr, T., & Brameld, J. M. (2021). Effect of AAV-mediated overexpression of ATF5 and downstream targets of an integrated stress response in murine skeletal muscle. Scientific Reports, 11(1), 1-13. https://doi.org/10.1038/s41598-021-99432-4

We previously reported that growth promoter-induced skeletal muscle hypertrophy co-ordinately upregulated expression of genes associated with an integrated stress response (ISR), as well as potential ISR regulators. We therefore used Adeno-Associated... Read More about Effect of AAV-mediated overexpression of ATF5 and downstream targets of an integrated stress response in murine skeletal muscle.

Effect of adeno-associated virus (AAV)-mediated overexpression of PEPCK-M (Pck2) on Clenbuterol-induced muscle growth (2019)
Journal Article
Loczenski-Brown, D. M., Jones, S., Luckett, J., Daniel, Z., Brearley, M. C., Ebling, F. J. P., …Brameld, J. M. (2019). Effect of adeno-associated virus (AAV)-mediated overexpression of PEPCK-M (Pck2) on Clenbuterol-induced muscle growth. PLoS ONE, 14(6), 1-12. https://doi.org/10.1371/journal.pone.0218970

We previously identified PEPCK-M (encoded by the Pck2 gene) to be highly up-regulated in skeletal muscle of pigs treated with Ractopamine, an anabolic beta-adrenergic receptor agonist. To determine whether PEPCK-M had a causative role in modulating t... Read More about Effect of adeno-associated virus (AAV)-mediated overexpression of PEPCK-M (Pck2) on Clenbuterol-induced muscle growth.

Effect of sodium 4-phenylbutyrate on clenbuterol-mediated muscle growth (2018)
Journal Article
Brown, D. M., Jones, S., Daniel, Z. C., Brearley, M. C., Lewis, J. E., Ebling, F. J., …Brameld, J. M. (2018). Effect of sodium 4-phenylbutyrate on clenbuterol-mediated muscle growth. PLoS ONE, 13(7), Article e0201481. https://doi.org/10.1371/journal.pone.0201481

Previously, we highlighted induction of an integrated stress response (ISR) gene program in skeletal muscle of pigs treated with a beta-adrenergic agonist. Hence we tested the hypothesis that the ER-stress inhibitor, sodium 4-phenylbutyrate (PBA), wo... Read More about Effect of sodium 4-phenylbutyrate on clenbuterol-mediated muscle growth.

Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain (2017)
Journal Article
Lewis, J. E., Brameld, J. M., Hill, P., Cocco, C., Noli, B., Ferri, G., …Jethwa, P. (2017). Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain. PLoS ONE, 12, Article e01727. https://doi.org/10.1371/journal.pone.0172724

VGF (non-acronymic) was first highlighted to have a role in energy homeostasis through experiments involving dietary manipulation in mice. Fasting increased VGF mRNA in the Arc and levels were subsequently reduced upon refeeding. This anabolic role f... Read More about Hypothalamic over-expression of VGF in the Siberian hamster increases energy expenditure and reduces body weight gain.

The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP (2015)
Journal Article
Lewis, J. E., Brameld, J. M., Hill, P., Barrett, P., Ebling, F. J., & Jethwa, P. (in press). The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP. Journal of Neuroscience Methods, 256, https://doi.org/10.1016/j.jneumeth.2015.08.013

Introduction: The viral 2A sequence has become an attractive alternative to the traditional internal ribosomal entry site (IRES) for simultaneous over-expression of two genes and in combination with recombinant adeno-associated viruses (rAAV) has bee... Read More about The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP.

Increased Responses to the Actions of Fibroblast Growth Factor 21 on Energy Balance and Body Weight in a Seasonal Model of Adiposity (2012)
Journal Article
Murphy, M., Samms, R., Warner, A., Bolborea, M., Barrett, P., Fowler, M. J., …Ebling, F. J. (2013). Increased Responses to the Actions of Fibroblast Growth Factor 21 on Energy Balance and Body Weight in a Seasonal Model of Adiposity. Journal of Neuroendocrinology, 25(2), 180-189. https://doi.org/10.1111/j.1365-2826.2012.02383.x

The present study aimed to investigate the actions of fibroblast growth factor 21 (FGF21) on energy balance in a natural model of relative fatness, the Siberian hamster. Hamsters were studied under long days (LD) to promote weight gain, or short days... Read More about Increased Responses to the Actions of Fibroblast Growth Factor 21 on Energy Balance and Body Weight in a Seasonal Model of Adiposity.

Short-days induce weight loss in siberian hamsters despite overexpression of the agouti-related peptide gene (2010)
Journal Article
Jethwa, P. H., Warner, A., Fowler, M. J., Murphy, M., de Backer, M. W., Adan, R. A., …Ebling, F. J. (2010). Short-days induce weight loss in siberian hamsters despite overexpression of the agouti-related peptide gene. Journal of Neuroendocrinology, 22(6), 564-575. https://doi.org/10.1111/j.1365-2826.2010.02001.x

Many vertebrates express profound annual cycles of body fattening, although it is not clear whether these represent differential activity of the central pathways known to mediate homeostatic control of food intake and energy expenditure, or whether t... Read More about Short-days induce weight loss in siberian hamsters despite overexpression of the agouti-related peptide gene.

Fetal exposure to a maternal low-protein diet during mid-gestation results in muscle-specific effects on fibre type composition in young rats (2007)
Journal Article
Mallinson, J. E., Sculley, D. V., Craigon, J., Plant, R., Langley-Evans, S. C., & Brameld, J. M. (2007). Fetal exposure to a maternal low-protein diet during mid-gestation results in muscle-specific effects on fibre type composition in young rats. British Journal of Nutrition, 98(2), 292-299. https://doi.org/10.1017/S0007114507701678

This study assessed the impact of reduced dietary protein during specific periods of fetal life upon muscle fibre development in young rats. Pregnant rats were fed a control or low-protein (LP) diet at early (days 0-7 gestation, LPEarly), mid (days 8... Read More about Fetal exposure to a maternal low-protein diet during mid-gestation results in muscle-specific effects on fibre type composition in young rats.

Thyrotrophin-releasing hormone decreases feeding and increases body temperature, activity and oxygen consumption in Siberian hamsters (2007)
Journal Article
Schuhler, S., Warner, A., Finney, N., Bennett, G. W., Ebling, F. J., & Brameld, J. M. (2007). Thyrotrophin-releasing hormone decreases feeding and increases body temperature, activity and oxygen consumption in Siberian hamsters. Journal of Neuroendocrinology, 19(4), 239-249. https://doi.org/10.1111/j.1365-2826.2006.01524.x

Thyrotrophin-releasing hormone (TRH) is known to play an important role in the control of food intake and energy metabolism in addition to its actions on the pituitary-thyroid axis. We have previously shown that central administration of TRH decrease... Read More about Thyrotrophin-releasing hormone decreases feeding and increases body temperature, activity and oxygen consumption in Siberian hamsters.