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Bacteriophage ZCKP1: a potential treatment for Klebsiella pneumoniae isolated from diabetic foot patients (2018)
Journal Article
Taha, O. A., Connerton, P., Connerton, I., & El-Shibiny, A. (2018). Bacteriophage ZCKP1: a potential treatment for Klebsiella pneumoniae isolated from diabetic foot patients. Frontiers in Microbiology, 9, doi:10.3389/fmicb.2018.02127

The recorded growth in infection by multidrug resistant bacteria necessitates prompt efforts towards developing alternatives to antibiotics, such as bacteriophage therapy. Immuno-compromised patients with diabetes mellitus are particularly prone to f... Read More

The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens (2018)
Journal Article
Connerton, P. L., Richards, P. J., Lafontaine, G. M., O'Kane, P. M., Ghaffar, N. M., Cummings, N. J., …Connerton, I. F. (in press). The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens. Microbiome, 6, doi:10.1186/s40168-018-0477-5. ISSN 2049-2618

Background Campylobacters are an unwelcome member of the poultry gut microbiota in terms of food safety. The objective of this study was to compare the microbiota, inflammatory responses, and zootechnical parameters of broiler chickens not exposed t... Read More

Characterisation of aerotolerant forms of a robust chicken colonizing Campylobacter coli (2017)
Journal Article
O'Kane, P. M., & Connerton, I. F. (in press). Characterisation of aerotolerant forms of a robust chicken colonizing Campylobacter coli. Frontiers in Microbiology, doi:10.3389/fmicb.2017.00513. ISSN 1664-302X

Campylobacter contaminated poultry meat is a major source of human foodborne illness. Campylobacter coli strain OR12 is a robust colonizer of chickens that was previously shown to outcompete and displace other Campylobacter strains from the chicken’s... Read More

The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection (2016)
Journal Article
Lis, L., & Connerton, I. F. (in press). The minor flagellin of Campylobacter jejuni (FlaB) confers defensive properties against bacteriophage infection. Frontiers in Microbiology, 7, doi:10.3389/fmicb.2016.01908. ISSN 1664-302X

A screen of bacteriophages infecting a panel of Campylobacter jejuni PT14 gene knock-out mutants identified a role for the minor flagellin encoded by the flaB gene, in the defense of the host against CP8unalikevirus bacteriophage CP_F1 infection. Ina... Read More

Campylobacters and their bacteriophages from chicken liver: the prospect for phage biocontrol (2016)
Journal Article
Firlieyanti, A. S., Connerton, P. L., & Connerton, I. F. (2016). Campylobacters and their bacteriophages from chicken liver: the prospect for phage biocontrol. International Journal of Food Microbiology, 237, doi:10.1016/j.ijfoodmicro.2016.08.026. ISSN 0168-1605

Consumption of foods containing chicken liver has been associated with Campylobacter enteritis. Campylobacters can contaminate the surface of livers post-mortem but can also arise through systemic infection of colonising bacteria in live birds. The u... Read More

The effect of thermal processing in oil on the macromolecular integrity and acrylamide formation from starch of three potato cultivars organically fertilized (2016)
Journal Article
Varzakas, T., Alghamdi, A., Alghamdi, H., Linforth, R. S., Dinu, V., Besong, T. D., …Israilides, C. (2016). The effect of thermal processing in oil on the macromolecular integrity and acrylamide formation from starch of three potato cultivars organically fertilized. Cogent Food and Agriculture, 2, doi:10.1080/23311932.2016.1180950. ISSN 2331-1932

Starches from three organically produced cultivars of potato tuber (Lady Rosetta, Spunta and Voyager) have been studied in relation to (i) acrylamide production (ii) macromolecular integrity after frying with extra virgin olive oil, soybean oil and c... Read More

The bacteriophage carrier state of Campylobacter jejuni features changes in host non-coding RNAs and the acquisition of new host-derived CRISPR spacer sequences (2016)
Journal Article
Hooton, S. P., Brathwaite, K. J., & Connerton, I. F. (2016). The bacteriophage carrier state of Campylobacter jejuni features changes in host non-coding RNAs and the acquisition of new host-derived CRISPR spacer sequences. Frontiers in Microbiology, 7(355), doi:10.3389/fmicb.2016.00355. ISSN 1664-302X

Incorporation of self-derived CRISPR DNA protospacers in Campylobacter jejuni PT14 occurs in the presence of bacteriophages encoding a CRISPR-like Cas4 protein. This phenomenon was evident in carrier state infections where both bacteriophages and ho... Read More

Pilot study of long-term anaesthesia in broiler chickens (2016)
Journal Article
O'Kane, P. M., Connerton, I. F., & White, K. L. (2016). Pilot study of long-term anaesthesia in broiler chickens. Veterinary Anaesthesia and Analgesia, 43(1), 72-75. doi:10.1111/vaa.12305

Objective: To provide stable anaesthesia of long duration in broiler chickens in order to perform a terminal caecal ligated loop procedure. Study design: Prospective experimental study. Animals: Seven clinically healthy broiler chickens (Gallus... Read More

Phase variation of a Type IIG restriction-modification enzyme alters site-specific methylation patterns and gene expression in Campylobacter jejuni strain NCTC11168 (2016)
Journal Article
Anjum, A., Kelly, B., Aidley, J. B., Connerton, P. L., Cummings, N. J., Parkhill, J., …Bayliss, C. D. (2016). Phase variation of a Type IIG restriction-modification enzyme alters site-specific methylation patterns and gene expression in Campylobacter jejuni strain NCTC11168. Nucleic Acids Research, doi:10.1093/nar/gkw019. ISSN 0305-1048

Phase-variable restriction-modification systems are a feature of a diverse range of bacterial species. Stochastic, reversible switches in expression of the methyltransferase produces variation in methylation of specific sequences. Phase-variable meth... Read More

Filamentation of Campylobacter in broth cultures (2015)
Journal Article
Ghaffar, N. M., Connerton, P. L., & Connerton, I. F. (2015). Filamentation of Campylobacter in broth cultures. Frontiers in Microbiology, 6(657), doi:10.3389/fmicb.2015.00657. ISSN 1664-302X

The transition from rod to filamentous cell morphology has been identified as a response to stressful conditions in many bacterial species and has been ascribed to confer certain survival advantages. Filamentation of Campylobacter jejuni was demonstr... Read More

Host adaption to the bacteriophage carrier state of Campylobacter jejuni (2015)
Journal Article
Brathwaite, K. J., Siringan, P., Connerton, P. L., & Connerton, I. F. (2015). Host adaption to the bacteriophage carrier state of Campylobacter jejuni. Research in Microbiology, doi:10.1016/j.resmic.2015.05.003. ISSN 0923-2508

The carrier state of the foodborne pathogen Campylobacter jejuni represents an alternative life cycle whereby virulent bacteriophage can persistent in association with host bacteria without commitment to lysogeny. Host bacteria exhibit significant ph... Read More

Xylan degrading enzymes from fungal sources (2015)
Journal Article
Kirikyali, N., & Connerton, I. F. (2015). Xylan degrading enzymes from fungal sources. Journal of Biocatalysis and Biotransformation, 4(1), doi:10.4172/10.4172/jpe.1000118. ISSN 2324-9099

Fungi have the ability to degrade xylan as the major component of plant cell wall hemicellulose. Fungi have evolved batteries of xylanolytic enzymes that concertedly act to depolymerise xylan backbones decorated with variable carbohydrate branches. A... Read More

Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein (2015)
Journal Article
Hooton, S. P., & Connerton, I. F. (2015). Campylobacter jejuni acquire new host-derived CRISPR spacers when in association with bacteriophages harboring a CRISPR-like Cas4 protein. Frontiers in Microbiology, 5(744), doi:10.3389/fmicb.2014.00744. ISSN 1664-302X

Campylobacter jejuni is a worldwide cause of human diarrhoeal disease. Clustered Repetitively Interspaced Palindromic Repeats (CRISPRs) and associated proteins allow Bacteria and Archaea to evade bacteriophage and plasmid infection. Type II CRISPR sy... Read More

The complete plasmid sequences of Salmonella enterica serovar Typhimurium U288 (2014)
Journal Article
Hooton, S. P., Timms, A. R., Cummings, N. J., Moreton, J., Wilson, R., & Connerton, I. F. (2014). The complete plasmid sequences of Salmonella enterica serovar Typhimurium U288. Plasmid, 76, 32-39. doi:10.1016/j.plasmid.2014.08.002

Salmonella enterica Serovar Typhimurium U288 is an emerging pathogen of pigs. The strain contains three plasmids of diverse origin that encode traits that are of concern for food security and safety, these include antibiotic resistant determinants, a... Read More

Alternative bacteriophage life cycles: the carrier state of Campylobacter jejuni (2014)
Journal Article
Siringan, P., Connerton, P. L., Cummmings, N. J., & Connerton, I. F. (2014). Alternative bacteriophage life cycles: the carrier state of Campylobacter jejuni. Open Biology, 4(3), doi:10.1098/rsob.130200. ISSN 2046-2441

Members of the genus Campylobacter are frequently responsible for human enteric disease, often through consumption of contaminated poultry products. Bacteriophages are viruses that have the potential to control pathogenic bacteria, but understanding... Read More

Complete genome sequence of universal bacteriophage host strain Campylobacter jejuni subsp. jejuni PT14 (2013)
Journal Article
Brathwaite, K. J., Siringan, P., Moreton, J., Wilson, R., & Connerton, I. F. (2013). Complete genome sequence of universal bacteriophage host strain Campylobacter jejuni subsp. jejuni PT14. Genome Announcements, 1(6), 1-2. doi:10.1128/genomea.00969-13

Campylobacter jejuni strain PT14 is a clinical isolate previously used to propagate bacteriophages in the United Kingdom phage typing scheme. The strain has proven useful in the isolation of Campylobacter bacteriophages from several sources, and it f... Read More