Research Repository

See what's under the surface


In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft (2018)
Journal Article
Rose, J., Sidney, L., Patient, J., White, L., Dua, H., El Haj, A., …Rose, F. (2018). In vitro evaluation of electrospun blends of gelatin and PCL for application as a partial thickness corneal graft. Journal of Biomedical Materials Research Part A, doi:10.1002/jbm.a.36598

The advent of innovative surgical procedures utilizing partial thickness corneal grafts has created a need for the development of synthetic implants to recreate corneal stromal tissue. This work evaluates electrospun gelatin and polycaprolactone (PCL... Read More

Localised corneal haze and scarring following pulsed accelerated collagen cross-linking for keratoconus (2018)
Journal Article
Said, D. G., Ross, A. R., Messina, M., Mohammed, I., & Dua, H. (2018). Localised corneal haze and scarring following pulsed accelerated collagen cross-linking for keratoconus. Eye, doi:10.1038/s41433-018-0211-3

Corneal collagen cross-linking (CXL) is the only treatment that delays or arrests progression of keratoconus. CXL is a safe procedure with few complications. CXL protocols vary depending on time and energy used but all are safe and effective. Variati... Read More

Elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling (2018)
Journal Article
Mohammed, I., Ross, A. R., Britton, J. O., Said, D. G., & Dua, H. S. (2018). Elastin content and distribution in endothelial keratoplasty tissue determines direction of scrolling. American Journal of Ophthalmology, 194, (16-25). doi:10.1016/j.ajo.2018.07.001. ISSN 0002-9394

Purpose Descemet membrane endothelial keratoplasty (DMEK) and pre-Descemet endothelial keratoplasty (PDEK) tissues always scroll with the endothelial cells (EC) outside. We designed a study to understand the reason for this behavior. Design... Read More

Nerve terminals at the human corneoscleral limbus (2017)
Journal Article
Al -Aqaba, M., Anis, F. S., Mohammed, I., & Dua, H. S. (2017). Nerve terminals at the human corneoscleral limbus. British Journal of Ophthalmology, 102(4), 556-561. doi:10.1136/bjophthalmol-2017-311146

Aims: To demonstrate and characterise distinct subepithelial compact nerve endings (CNE) at the human corneoscleral limbus. Methods: Ten fresh human donor corneoscleral discs (mean age, 67 years) and 26 organ-cultured corneoscleral rims (mean age,... Read More

Air pressure changes in the creation and bursting of the type-1 big bubble in deep anterior lamellar keratoplasty: an ex vivo study (2017)
Journal Article
AlTaan, S., Mohammed, I., Said, D., & Dua, H. (2018). Air pressure changes in the creation and bursting of the type-1 big bubble in deep anterior lamellar keratoplasty: an ex vivo study. Eye, 32(1), 146-151. doi:10.1038/eye.2017.121

Purpose: To measure the pressure and volume of air required to create a big bubble (BB) in simulated deep anterior lamellar keratoplasty (DALK) in donor eyes and ascertain the bursting pressure of the BB. Patients and methods: Twenty-two human scler... Read More

Human antimicrobial peptides in ocular surface defense (2017)
Journal Article
Mohammed, I., Said, D. G., & Dua, H. S. (2017). Human antimicrobial peptides in ocular surface defense. Progress in Retinal and Eye Research, 61, (1-22). doi:10.1016/j.preteyeres.2017.03.004. ISSN 1350-9462

Sight depends on the passage of light through the transparent cornea and being focused on the fovea. Its exposed position renders it vulnerable to microbial infection. The cornea has developed a wide array of defense mechanisms against infection, of... Read More

3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review (2017)
Journal Article
Prina, E., Mistry, P., Sidney, L. E., Yang, J., Wildman, R. D., Bertolin, M., …Rose, F. R. A. (in press). 3D microfabricated scaffolds and microfluidic devices for ocular surface replacement: a review. Stem Cell Reviews and Reports, doi:10.1007/s12015-017-9740-6. ISSN 1550-8943

In recent years, there has been increased research interest in generating corneal substitutes, either for use in the clinic or as in vitro corneal models. The advancement of 3D microfabrication technologies has allowed the reconstruction of the nativ... Read More

Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells (2015)
Journal Article
Sidney, L. E., Branch, M. J., Dua, H. S., & Hopkinson, A. (2015). Effect of culture medium on propagation and phenotype of corneal stroma-derived stem cells. Cytotherapy, 17(12), doi:10.1016/j.jcyt.2015.08.003. ISSN 1465-3249

Background: The limbal area of the corneal stroma has been identified as a source of mesenchymal-like stem cells, which have potential for exploitation as a cell therapy. However, the optimal culture conditions are disputed and few direct media compa... Read More

Corneal decellularization: a method of recycling unsuitable donor tissue for clinical translation? (2015)
Journal Article
Wilson, S. L., Sidney, L. E., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2016). Corneal decellularization: a method of recycling unsuitable donor tissue for clinical translation?. Current Eye Research, 41(6), (769-782). doi:10.3109/02713683.2015.1062114. ISSN 0271-3683

Background: There is a clinical need for biomimetic corneas that are as effective, preferably superior, to cadaveric donor tissue. Decellularized tissues are advantageous compared to synthetic or semi-synthetic engineered tissues in that the native m... Read More

Clinical evaluation and characterisation of corneal vascularisation (2015)
Journal Article
Faraj, L., Said, D. G., AL AQABA, M., Otri, A. M., & Dua, H. S. (2016). Clinical evaluation and characterisation of corneal vascularisation. British Journal of Ophthalmology, 100(3), 315-322. doi:10.1136/bjophthalmol-2015-306686

Background/aims: To clinically characterise corneal neovascularisation (CVas) with a view to elaborate clinical presentations and to standardise descriptors for clinical evaluation and research. Methods: Corneas of 165 patients with CVas due to a va... Read More

Concise review: evidence for CD34 as a common marker for diverse progenitors (2014)
Journal Article
Sidney, L. E., Branch, M. J., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2014). Concise review: evidence for CD34 as a common marker for diverse progenitors. STEM CELLS, 32(6), (1380-1389). doi:10.1002/stem.1661. ISSN 1066-5099

CD34 is a transmembrane phosphoglycoprotein, first identified on hematopoietic stem and progenitor cells. Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. Due to these historical... Read More

A novel antimicrobial peptide on the ocular surface shows decreased expression in inflammation and infection (2008)
Journal Article
Abedin, A., Mohammed, I., Hopkinson, A., & Dua, H. S. (2008). A novel antimicrobial peptide on the ocular surface shows decreased expression in inflammation and infection. Investigative Ophthalmology & Visual Science, 49(1), (28-33). doi:10.1167/iovs.07-0645. ISSN 1552-5783

PURPOSE: Antimicrobial peptides (AMPs) are cationic host defense peptides with microbicidal and cell-signaling properties. They show promise as potential therapeutic agents. In the present study, a beta-defensin AMP gene was isolated from the ocular... Read More

Optimised laser microdissection of the human ocular surface epithelial regions for microarray studies
Journal Article
Kulkarn, B. B. .., Powe, D. G., Hopkinson, A., & Dua, H. S. Optimised laser microdissection of the human ocular surface epithelial regions for microarray studies. BMC Ophthalmology, 13, doi:10.1186/1471-2415-13-62. ISSN 1471-2415

Background The most important challenge of performing insitu transcriptional profiling of the human ocular surface epithelial regions is obtaining samples in sufficient amounts, without contamination from adjacent tissue, as the region of interest i... Read More