All Outputs (10)

Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis (2016)
Journal Article
Harding, S. E., Gillis, R. B., & Adams, G. G. (in press). Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis. Biophysical Reviews, 8(4), https://doi.org/10.1007/s12551-016-0232-8

Molecular weights (molar masses), molecular weight distributions, dissociation constants and other interaction parameters are fundamental characteristics of proteins, nucleic acids, polysaccharides and glycoconjugates in solution. Sedimentation equil... Read More about Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis.

Dietitians' practice in giving carbohydrate advice in the management of type 2 diabetes: a mixed methods study (2016)
Journal Article
McArdle, P. D., Greenfield, S. M., Avery, A., Adams, G. G., & Gill, P. S. (in press). Dietitians' practice in giving carbohydrate advice in the management of type 2 diabetes: a mixed methods study. Journal of Human Nutrition and Dietetics, 30(3), https://doi.org/10.1111/jhn.12436

Background: Carbohydrate is accepted as the principal nutrient affecting blood glucose in diabetes; however, current guidelines are unable to specify the optimal quantity of carbohydrate for glycaemic control. No studies exist that describe current p... Read More about Dietitians' practice in giving carbohydrate advice in the management of type 2 diabetes: a mixed methods study.

Solution conformation and flexibility of capsular polysaccharides from Neisseria meningitidis and glycoconjugates with the tetanus toxoid protein (2016)
Journal Article
Abdelhameed, A. S., Morris, G. A., Almutairi, F., Adams, G. G., Duvivier, P., Conrath, K., & Harding, S. E. (in press). Solution conformation and flexibility of capsular polysaccharides from Neisseria meningitidis and glycoconjugates with the tetanus toxoid protein. Scientific Reports, 6(1), 1-11. https://doi.org/10.1038/srep35588

The structural integrity of meningococcal native, micro-fluidized and activated capsular polysaccharides and their glycoconjugates – in the form most relevant to their potential use as vaccines (dilute solution) - have been investigated with respect... Read More about Solution conformation and flexibility of capsular polysaccharides from Neisseria meningitidis and glycoconjugates with the tetanus toxoid protein.

Both reversible self-association and structural changes underpin molecular viscoelasticity of mAb solutions (2016)
Journal Article
Sarangapani, P. S., Weaver, J., Parupudi, A., Besong, T. M., Adams, G. G., Harding, S. E., …Pathak, J. A. (2016). Both reversible self-association and structural changes underpin molecular viscoelasticity of mAb solutions. Journal of Pharmaceutical Sciences, 105(12), https://doi.org/10.1016/j.xphs.2016.08.020

The role of antibody structure (conformation) in solution rheology is probed. It is demonstrated here that pH-dependent changes in the tertiary structure of 2 mAb solutions lead to viscoelasticity and not merely a shear viscosity (η) increase. Steady... Read More about Both reversible self-association and structural changes underpin molecular viscoelasticity of mAb solutions.

Genetic improvement of tomato by targeted control of fruit softening (2016)
Journal Article
Uluisik, S., Chapman, N. H., Smith, R., Poole, M., Adams, G., Gillis, R. B., …Seymour, G. B. (2016). Genetic improvement of tomato by targeted control of fruit softening. Nature Biotechnology, 34, 950-952. https://doi.org/10.1038/nbt.3602

Controlling the rate of softening to extend shelf life was a key target for researchers engineering genetically modified (GM) tomatoes in the 1990s, but only modest improvements were achieved. Hybrids grown nowadays contain 'non-ripening mutations' t... Read More about Genetic improvement of tomato by targeted control of fruit softening.

The effect of thermal processing in oil on the macromolecular integrity and acrylamide formation from starch of three potato cultivars organically fertilized (2016)
Journal Article
Varzakas, T., Alghamdi, A., Alghamdi, H., Linforth, R. S., Dinu, V., Besong, T. D., …Israilides, C. (2016). The effect of thermal processing in oil on the macromolecular integrity and acrylamide formation from starch of three potato cultivars organically fertilized. Cogent Food and Agriculture, 2(1), https://doi.org/10.1080/23311932.2016.1180950

Starches from three organically produced cultivars of potato tuber (Lady Rosetta, Spunta and Voyager) have been studied in relation to (i) acrylamide production (ii) macromolecular integrity after frying with extra virgin olive oil, soybean oil and c... Read More about The effect of thermal processing in oil on the macromolecular integrity and acrylamide formation from starch of three potato cultivars organically fertilized.

Molecular weight analysis of starches: which technique? (2016)
Journal Article
Harding, S. E., Adams, G. G., & Gillis, R. B. (2016). Molecular weight analysis of starches: which technique?. Starch/Stärke, 68(9-10), https://doi.org/10.1002/star.201600042

A number of methods are available to researchers for estimating molecular weights (molar masses) and molecular weight distributions of starches ? or those in solution, using an appropriate solvent/solubilisation protocol. We outline the methods avail... Read More about Molecular weight analysis of starches: which technique?.

Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides (2016)
Book Chapter
Harding, S. E., Adams, G. G., Gillis, R. B., Almutairi, F. M., & Morris, G. A. (2016). Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides. In S. Uchiyama, F. Arisaka, W. F. Stafford, & T. M. Laue (Eds.), Analytical ultracentrifugation: instrumentation, software and applications (375-386). Springer. https://doi.org/10.1007/978-4-431-55985-6_18

Molar mass or ‘molecular weight’ is one of the most fundamental parameters describing a macromolecule. Because of their polydisperse nature, polysaccharides are usually described by distributions of molar mass. SEC-MALS (size exclusion chromatography... Read More about Aspects of the analytical ultracentrifuge determination of the molar mass distribution of polysaccharides.

A glycoconjugate of Haemophilus influenzae Type b capsular polysaccharide with tetanus toxoid protein: hydrodynamic properties mainly influenced by the carbohydrate (2016)
Journal Article
Abdelhameed, A. S., Adams, G. G., Morris, G. A., Almutairi, F. M., Duvivier, P., Conrath, K., & Harding, S. E. (2016). A glycoconjugate of Haemophilus influenzae Type b capsular polysaccharide with tetanus toxoid protein: hydrodynamic properties mainly influenced by the carbohydrate. Scientific Reports, 6(1), Article 22208. https://doi.org/10.1038/srep22208

Three important physical properties which may affect the performance of glycoconjugate vaccines against serious disease are molar mass (molecular weight), heterogeneity (polydispersity), and conformational flexibility in solution. The dilute solution... Read More about A glycoconjugate of Haemophilus influenzae Type b capsular polysaccharide with tetanus toxoid protein: hydrodynamic properties mainly influenced by the carbohydrate.

Coiled coil type neoglycoproteins presenting three lactose residues (2016)
Journal Article
Sweeney, S. M., Bullen, G. A., Gillis, R. B., Adams, G. G., Rowe, A. J., Harding, S. E., …Murphy, P. V. (2016). Coiled coil type neoglycoproteins presenting three lactose residues. Tetrahedron Letters, 57(13), https://doi.org/10.1016/j.tetlet.2016.02.005

Scaffold design, synthesis and application are relevant for biomedical research. For example, multivalent interactions, such as those between cell surface glycoproteins and lectins can influence the potency and duration of signalling. The spacing bet... Read More about Coiled coil type neoglycoproteins presenting three lactose residues.