Research Repository

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Polyionic tags as enhancers of protein solubility in recombinant protein expression (2018)
Journal Article
Paraskevopoulou, V., & Falcone, F. (2018). Polyionic tags as enhancers of protein solubility in recombinant protein expression. Microorganisms, 6(2), 47. doi:10.3390/microorganisms6020047

Since the introduction of recombinant protein expression in the second half of the 1970s, the growth of the biopharmaceutical field has been rapid and protein therapeutics has come to the foreground. Biophysical and structural characterisation of rec... Read More

RBL cells as models for in vitro studies of mast cells and basophils (2018)
Journal Article
Falcone, F. H., Wan, D., Barwary, N., & Sagi-Eisenberg, R. (2018). RBL cells as models for in vitro studies of mast cells and basophils. Immunological Reviews, 282(1), doi:10.1111/imr.12628. ISSN 0105-2896

Since their establishment in 1981, RBL-2H3 cells have been widely used as a mast cell (MC) model. Their ability to be easily grown in culture in large amounts, their responsiveness to FcεRI-mediated triggers and the fact that they can be genetically... Read More

Structural basis of Lewis(b) antigen binding by the Helicobacter pylori adhesin BabA (2015)
Journal Article
Hage, N., Howard, T., Phillips, C., Brassington, C., Overman, R., Debreczeni, J., …Falcone, F. H. (2015). Structural basis of Lewis(b) antigen binding by the Helicobacter pylori adhesin BabA. Science Advances, 1(7), doi:10.1126/sciadv.1500315

Helicobacter pylori is a leading cause of peptic ulceration and gastric cancer worldwide. To achieve colonization of the stomach, this Gram-negative bacterium adheres to Lewis(b) (Le(b)) antigens in the gastric mucosa using its outer membrane protein... Read More

Serological screening of the Schistosoma mansoni adult worm proteome (2014)
Journal Article
Falcone, F., Ludolf, F., Patrocínio, P. R., Corrêa-Oliveira, R., Gazzinelli, A., Teixeira-Ferreira, A., …Silva-Pereira, R. A. (2014). Serological screening of the Schistosoma mansoni adult worm proteome. PLoS Neglected Tropical Diseases, 8(3), 1-16. doi:10.1371/journal.pntd.0002745

Background New interventions tools are a priority for schistosomiasis control and elimination, as the disease is still highly prevalent. The identification of proteins associated with active infection and protective immune response may constitute th... Read More

Basement membrane influences intestinal epithelial cell growth and presents a barrier to the movement of macromolecules (2014)
Journal Article
Stolnik-Trenkic, S., Vllasaliua, D., Falcone, F. H., & Garnett, M. (2014). Basement membrane influences intestinal epithelial cell growth and presents a barrier to the movement of macromolecules. Experimental Cell Research, 323(1), 218-231. doi:10.1016/j.yexcr.2014.02.022

This work examines the potential drug delivery barrier of the basement membrane (BM) by assessing the permeability of select macromolecules and nanoparticles. The study further extends to probing the effect of BM on intestinal epithelial cell attachm... Read More