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Use of BODIPY-Labeled ATP Analogues in the Development and Validation of a Fluorescence Polarization-Based Assay for Screening of Kinase Inhibitors (2020)
Journal Article
Moreira, B. P., Armstrong, T., Batista, I. C. A., Clemente Tavares, N., Pires, C. V., de Moraes Mourão, M., …Dekker, L. V. (2020). Use of BODIPY-Labeled ATP Analogues in the Development and Validation of a Fluorescence Polarization-Based Assay for Screening of Kinase Inhibitors. ACS Omega, 5(16), 9064-9070. https://doi.org/10.1021/acsomega.9b03344

Copyright © 2020 American Chemical Society. The screening of compound libraries to identify small-molecule modulators of specific biological targets is crucial in the process for the discovery of novel therapeutics and molecular probes. Considering t... Read More about Use of BODIPY-Labeled ATP Analogues in the Development and Validation of a Fluorescence Polarization-Based Assay for Screening of Kinase Inhibitors.

Characterization of human FcεRIα chain expression and gene copy number in humanized rat basophilic leukaemia (RBL) reporter cell lines (2019)
Journal Article
Ali Ali, E., Kalli, M., Wan, D., Nakamura, R., Onion, D., Alanine, D. G., …Falcone, F. H. (2019). Characterization of human FcεRIα chain expression and gene copy number in humanized rat basophilic leukaemia (RBL) reporter cell lines. PLoS ONE, 14(8), Article e0221034. https://doi.org/10.1371/journal.+pone.0221034

Several laboratories have created rat basophil leukemia (RBL) cell lines stably transfected with the human high affinity IgE receptor (FcεRI H). More recently, humanized RBL cell lines saw the introduction of reporter genes such as luciferase (RS-ATL... Read More about Characterization of human FcεRIα chain expression and gene copy number in humanized rat basophilic leukaemia (RBL) reporter cell lines.

Introduction of a C-terminal hexa-lysine tag increases thermal stability of the LacDiNac binding adhesin (LabA) exodomain from Helicobacter pylori (2019)
Journal Article
Paraskevopoulou, V., Artiaga, V. G., Rowlinson, R., Winkler, G. S., Gellert, P., Stolnik, S., …Falcone, F. H. (2019). Introduction of a C-terminal hexa-lysine tag increases thermal stability of the LacDiNac binding adhesin (LabA) exodomain from Helicobacter pylori. Protein Expression and Purification, 163, 1-8. https://doi.org/10.1016/j.pep.2019.105446

Helicobacter pylori is a pathogenic microorganism infecting approximately 50% of the global population, and establishes life-long colonization despite the hostile stomach environment. H. pylori employs a wide range of outer membrane proteins (adhesin... Read More about Introduction of a C-terminal hexa-lysine tag increases thermal stability of the LacDiNac binding adhesin (LabA) exodomain from Helicobacter pylori.

The Schistosoma mansoni cyclophilin A epitope 107-121 induces a protective immune response against schistosomiasis (2019)
Journal Article
de Melo, T. T., Mendes, M. M., Alves, C. C., Carvalho, G. B., Fernandes, V. C., Pimenta, D. L. F., …Fonseca, C. T. (2019). The Schistosoma mansoni cyclophilin A epitope 107-121 induces a protective immune response against schistosomiasis. Molecular Immunology, 111, 172-181. https://doi.org/10.1016/j.molimm.2019.04.021

Great efforts have been made to identify promising antigens and vaccine formulations against schistosomiasis. Among the previously described Schistosoma vaccine candidates, cyclophilins comprise an interesting antigen that could be used for vaccine f... Read More about The Schistosoma mansoni cyclophilin A epitope 107-121 induces a protective immune response against schistosomiasis.

IPSE, a parasite-derived host immunomodulatory protein, is a potential therapeutic for hemorrhagic cystitis (2019)
Journal Article
Zee, R. S., Mbanefo, E. C., Le, L. H., Pennington, L. F., Odegaard, J., Jardetzky, T. S., …Hsieh, M. H. (2019). IPSE, a parasite-derived host immunomodulatory protein, is a potential therapeutic for hemorrhagic cystitis. AJP - Renal Physiology, 316(6), F1133-F1140. https://doi.org/10.1152/ajprenal.00468.2018

Chemotherapy-induced hemorrhagic cystitis is characterized by bladder pain and voiding dysfunction caused by hemorrhage and inflammation. Novel therapeutic options to treat hemorrhagic cystitis are needed. We previously reported that systemic adminis... Read More about IPSE, a parasite-derived host immunomodulatory protein, is a potential therapeutic for hemorrhagic cystitis.

IPSE, a urogenital parasite-derived immunomodulatory protein, ameliorates ifosfamide-induced hemorrhagic cystitis through downregulation of pro-inflammatory pathways (2019)
Journal Article
Mbanefo, E. C., Le, L., Zee, R., Banskota, N., Ishida, K., Pennington, L. F., …Hsieh, M. H. (2019). IPSE, a urogenital parasite-derived immunomodulatory protein, ameliorates ifosfamide-induced hemorrhagic cystitis through downregulation of pro-inflammatory pathways. Scientific Reports, 9, Article 1586. https://doi.org/10.1038/s41598-018-38274-z

Ifosfamide and other oxazaphosphorines can result in hemorrhagic cystitis, a constellation of complications caused by acrolein metabolites. We previously showed that a single dose of IPSE (Interleukin-4-inducing principle from Schistosoma eggs), a sc... Read More about IPSE, a urogenital parasite-derived immunomodulatory protein, ameliorates ifosfamide-induced hemorrhagic cystitis through downregulation of pro-inflammatory pathways.

Therapeutic exploitation of IPSE, a urogenital parasite-derived host modulatory protein, for chemotherapy-induced hemorrhagic cystitis (2018)
Journal Article
Mbanefo, E. C., Le, L., Pennington, L. F., Odegaard, J. I., Jardetzky, T. S., Alouffi, A., …Hsieh, M. H. (2018). Therapeutic exploitation of IPSE, a urogenital parasite-derived host modulatory protein, for chemotherapy-induced hemorrhagic cystitis. FASEB Journal, 32(8), 4408-4419. https://doi.org/10.1096/fj.201701415r

Chemotherapy-induced hemorrhagic cystitis (CHC) can be difficult to manage. Prior work suggests that IL-4 alleviates ifosfamide-induced hemorrhagic cystitis (IHC), but systemically administered IL-4 causes significant side effects. We hypothesized th... Read More about Therapeutic exploitation of IPSE, a urogenital parasite-derived host modulatory protein, for chemotherapy-induced hemorrhagic cystitis.

Polyionic tags as enhancers of protein solubility in recombinant protein expression (2018)
Journal Article
Paraskevopoulou, V., & Falcone, F. (2018). Polyionic tags as enhancers of protein solubility in recombinant protein expression. Microorganisms, 6(2), 47. https://doi.org/10.3390/microorganisms6020047

Since the introduction of recombinant protein expression in the second half of the 1970s, the growth of the biopharmaceutical field has been rapid and protein therapeutics has come to the foreground. Biophysical and structural characterisation of rec... Read More about Polyionic tags as enhancers of protein solubility in recombinant protein expression.

RBL cells as models for in vitro studies of mast cells and basophils (2018)
Journal Article
Falcone, F. H., Wan, D., Barwary, N., & Sagi-Eisenberg, R. (2018). RBL cells as models for in vitro studies of mast cells and basophils. Immunological Reviews, 282(1), 47-57. https://doi.org/10.1111/imr.12628

Since their establishment in 1981, RBL-2H3 cells have been widely used as a mast cell (MC) model. Their ability to be easily grown in culture in large amounts, their responsiveness to FcεRI-mediated triggers and the fact that they can be genetically... Read More about RBL cells as models for in vitro studies of mast cells and basophils.

Dry-powder formulations of non-covalent protein complexes with linear or miktoarm copolymers for pulmonary delivery (2018)
Journal Article
Nieto-Orellana, A., Coghlan, D., Rothery, M., Falcone, F. H., Bosquillon, C., Childerhouse, N., …Stolnik, S. (2018). Dry-powder formulations of non-covalent protein complexes with linear or miktoarm copolymers for pulmonary delivery. International Journal of Pharmaceutics, 540(1-2), https://doi.org/10.1016/j.ijpharm.2018.02.008

Pulmonary delivery of protein therapeutics has considerable clinical potential for treating both local and systemic diseases. However, poor protein conformational stability, immunogenicity and protein degradation by proteolytic enzymes in the lung ar... Read More about Dry-powder formulations of non-covalent protein complexes with linear or miktoarm copolymers for pulmonary delivery.

Screening natural libraries of human milk oligosaccharides against lectins using CaR-ESI-MS (2017)
Journal Article
El-Hawiet, A., Chen, Y., Shams-Ud-Doha, K., Kitova, E. N., Kitov, P. I., Bode, L., …Klassen, J. S. (2018). Screening natural libraries of human milk oligosaccharides against lectins using CaR-ESI-MS. Analyst, 143(2), 536-548. https://doi.org/10.1039/c7an01397c

© 2018 The Royal Society of Chemistry. Human milk oligosaccharides (HMOs) afford many health benefits to breast-fed infants, such as protection against infection and regulation of the immune system, through the formation of non-covalent interactions... Read More about Screening natural libraries of human milk oligosaccharides against lectins using CaR-ESI-MS.

H-IPSE is a pathogen-secreted host nucleus-infiltrating protein (Infiltrin) expressed exclusively by the Schistosoma haematobium egg stage (2017)
Journal Article
Pennington, L. F., Alouffi, A., Mbanefo, E. C., Ray, D., Heery, D. M., Jardetzky, T. S., …Falcone, F. H. (2017). H-IPSE is a pathogen-secreted host nucleus-infiltrating protein (Infiltrin) expressed exclusively by the Schistosoma haematobium egg stage. Infection and Immunity, 85(12), Article e00301-17. https://doi.org/10.1128/iai.00301-17

Urogenital schistosomiasis, caused by the parasitic trematode Schistosoma haematobium, affects over 112 million people worldwide. As with Schistosoma mansoni infections, the pathology of urogenital schistosomiasis is related mainly to the egg stage,... Read More about H-IPSE is a pathogen-secreted host nucleus-infiltrating protein (Infiltrin) expressed exclusively by the Schistosoma haematobium egg stage.

Effect of polymer topology on non-covalent polymer-protein complexation: miktoarm versus linear mPEG-poly(glutamic acid) copolymers (2017)
Journal Article
Nieto-Orellana, A., Di Antonio, M., Conte, C., Falcone, F. H., Bosquillon, C., Childerhouse, N., …Stolnik, S. (in press). Effect of polymer topology on non-covalent polymer-protein complexation: miktoarm versus linear mPEG-poly(glutamic acid) copolymers. Polymer Chemistry, 8(14), https://doi.org/10.1039/C7PY00169J

Non-covalent polymer-protein conjugation is emerging as a potential route to improve pharmacokinetics and pharmacodynamics of protein therapeutics. In this study, a family of structurally related block copolymers of mPEG2k - poly(glutamic acid) with... Read More about Effect of polymer topology on non-covalent polymer-protein complexation: miktoarm versus linear mPEG-poly(glutamic acid) copolymers.

Live imaging of cellular internalization of single colloidal particle by combined label-free and fluorescence total internal reflection microscopy (2015)
Journal Article
Byrne, G. D., Vllasaliu, D., Falcone, F. H., Somekh, M. G., & Stolnik, S. (2015). Live imaging of cellular internalization of single colloidal particle by combined label-free and fluorescence total internal reflection microscopy. Molecular Pharmaceutics, 12(11), 3862-3870. doi:10.1021/acs.molpharmaceut.5b00215

In this work we utilize the combination of label-free total internal reflection microscopy and total internal reflectance fluorescence (TIRM/TIRF) microscopy to achieve a simultaneous, live imaging of single, label-free colloidal particle endocytosis... Read More about Live imaging of cellular internalization of single colloidal particle by combined label-free and fluorescence total internal reflection microscopy.

Structural basis of Lewis(b) antigen binding by the Helicobacter pylori adhesin BabA (2015)
Journal Article
Hage, N., Howard, T., Phillips, C., Brassington, C., Overman, R., Debreczeni, J., …Falcone, F. H. (2015). Structural basis of Lewis(b) antigen binding by the Helicobacter pylori adhesin BabA. Science Advances, 1(7), Article e1500315. https://doi.org/10.1126/sciadv.1500315

Helicobacter pylori is a leading cause of peptic ulceration and gastric cancer worldwide. To achieve colonization of the stomach, this Gram-negative bacterium adheres to Lewis(b) (Le(b)) antigens in the gastric mucosa using its outer membrane protein... Read More about Structural basis of Lewis(b) antigen binding by the Helicobacter pylori adhesin BabA.

Improved expression and purification of the Helicobacter pylori adhesin BabA through the incorporation of a hexa-lysine tag (2014)
Journal Article
Hage, N., Falcone, F., Renshaw, J. G., Sebastiaan Winkler, G., Gellert, P., & Stolnik, S. (2015). Improved expression and purification of the Helicobacter pylori adhesin BabA through the incorporation of a hexa-lysine tag. Protein Expression and Purification, 106, 25-30. doi:10.1016/j.pep.2014.10.009

Helicobacter pylori is a pathogenic bacterium that has the remarkable ability to withstand the harsh conditions of the stomach for decades. This is achieved through unique evolutionary adaptations, which include binding Lewisb antigens found on the g... Read More about Improved expression and purification of the Helicobacter pylori adhesin BabA through the incorporation of a hexa-lysine tag.

Use of humanised rat basophilic leukaemia cell line RSATL8 for the assessment of allergenicity of Schistosoma mansoni proteins (2014)
Journal Article
Alcocer, M., Wan, D., Ludolf, F., Alanine, D. G. W., Stretton, O., Ali Ali, E., …Falcone, F. H. (2014). Use of humanised rat basophilic leukaemia cell line RSATL8 for the assessment of allergenicity of Schistosoma mansoni proteins. PLoS Neglected Tropical Diseases, 8(9), 1-13. https://doi.org/10.1371/journal.pntd.0003124

Background Parasite-specific IgE is thought to correlate with protection against Schistosoma mansoni infection or re-infection. Only a few molecular targets of the IgE response in S. mansoni infection have been characterised. A better insight into... Read More about Use of humanised rat basophilic leukaemia cell line RSATL8 for the assessment of allergenicity of Schistosoma mansoni proteins.

High prevalence of chitotriosidase deficiency in Peruvian Amerindians exposed to chitin-bearing food and enteroparasites (2014)
Journal Article
Manno, N., Sherratt, S., Boaretto, F., Mejìa Coico, F., Espinoza Camus, C., Jara Campos, C., …Falcone, F. (2014). High prevalence of chitotriosidase deficiency in Peruvian Amerindians exposed to chitin-bearing food and enteroparasites. Carbohydrate Polymers, 113, 607-614. https://doi.org/10.1016/j.carbpol.2014.07.011

The human genome encodes a gene for an enzymatically active chitinase (CHIT1) located in a single copy on Chromosome 1, which is highly expressed by activated macrophages and in other cells of the innate immune response. Several dysfunctional mutatio... Read More about High prevalence of chitotriosidase deficiency in Peruvian Amerindians exposed to chitin-bearing food and enteroparasites.

Serological screening of the Schistosoma mansoni adult worm proteome (2014)
Journal Article
Falcone, F., Ludolf, F., Patrocínio, P. R., Corrêa-Oliveira, R., Gazzinelli, A., Teixeira-Ferreira, A., …Silva-Pereira, R. A. (2014). Serological screening of the Schistosoma mansoni adult worm proteome. PLoS Neglected Tropical Diseases, 8(3), 1-16. https://doi.org/10.1371/journal.pntd.0002745

Background New interventions tools are a priority for schistosomiasis control and elimination, as the disease is still highly prevalent. The identification of proteins associated with active infection and protective immune response may constitute th... Read More about Serological screening of the Schistosoma mansoni adult worm proteome.