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Plasmodium SAS4: basal body component of male cell which is dispensable for parasite transmission (2022)
Journal Article
Zeeshan, M., Brady, D., Markus, R., Vaughan, S., Ferguson, D., Holder, A. A., & Tewari, R. (2022). Plasmodium SAS4: basal body component of male cell which is dispensable for parasite transmission. Life Science Alliance, 5(9), Article e202101329. https://doi.org/10.26508/lsa.202101329

The centriole/basal body (CBB) is an evolutionarily conserved organelle acting as a microtubule organising centre (MTOC) to nucleate cilia, flagella, and the centrosome. SAS4/CPAP is a conserved component associated with BB biogenesis in many model f... Read More about Plasmodium SAS4: basal body component of male cell which is dispensable for parasite transmission.

Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages (2021)
Journal Article
Zeeshan, M., Pandey, R., Subudhi, A. K., Ferguson, D. J., Kaur, G., Rashpa, R., …Tewari, R. (2021). Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages. Communications Biology, 4(1), Article 760. https://doi.org/10.1038/s42003-021-02273-0

PP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the malaria parasite life cycle incl... Read More about Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages.

MRE11 Is Crucial for Malaria Parasite Transmission and Its Absence Affects Expression of Interconnected Networks of Key Genes Essential for Life (2020)
Journal Article
Guttery, D. S., Ramaprasad, A., Ferguson, D. J. P., Zeeshan, M., Pandey, R., Brady, D., …Tewari, R. (2020). MRE11 Is Crucial for Malaria Parasite Transmission and Its Absence Affects Expression of Interconnected Networks of Key Genes Essential for Life. Cells, 9(12), Article 2590. https://doi.org/10.3390/cells9122590

The meiotic recombination 11 protein (MRE11) plays a key role in DNA damage response and maintenance of genome stability. However, little is known about its function during development of the malaria parasite Plasmodium. Here, we present a functional... Read More about MRE11 Is Crucial for Malaria Parasite Transmission and Its Absence Affects Expression of Interconnected Networks of Key Genes Essential for Life.

Plasmodium berghei Kinesin-5 Associates With the Spindle Apparatus During Cell Division and Is Important for Efficient Production of Infectious Sporozoites (2020)
Journal Article
Zeeshan, M., Brady, D., Stanway, R. R., Moores, C., Holder, A. A., & Tewari, R. (2020). Plasmodium berghei Kinesin-5 Associates With the Spindle Apparatus During Cell Division and Is Important for Efficient Production of Infectious Sporozoites. Frontiers in Cellular and Infection Microbiology, 10, Article 583812. https://doi.org/10.3389/fcimb.2020.583812

© Copyright © 2020 Zeeshan, Brady, Stanway, Moores, Holder and Tewari. Kinesin-5 motors play essential roles in spindle apparatus assembly during cell division, by generating forces to establish and maintain the spindle bipolarity essential for prope... Read More about Plasmodium berghei Kinesin-5 Associates With the Spindle Apparatus During Cell Division and Is Important for Efficient Production of Infectious Sporozoites.

A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission (2020)
Journal Article
Balestra, A. C., Zeeshan, M., Rea, E., Pasquarello, C., Brusini, L., Mourier, T., …Brochet, M. (2020). A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission. eLife, 9, Article e56474. https://doi.org/10.7554/eLife.56474

© 2020, Balestra et al. Cell cycle transitions are generally triggered by variation in the activity of cyclin-dependent kinases (CDKs) bound to cyclins. Malaria-causing parasites have a life cycle with unique cell-division cycles, and a repertoire of... Read More about A divergent cyclin/cyclin-dependent kinase complex controls the atypical replication of a malaria parasite during gametogony and transmission.

Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation (2020)
Journal Article
Zeeshan, M., Pandey, R., Ferguson, D. J. P., Tromer, E. C., Markus, R., Abel, S., …Tewari, R. (2020). Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation. Journal of Cell Science, 134(5), Article jcs.245753. https://doi.org/10.1242/jcs.245753

Eukaryotic cell proliferation requires chromosome replication and precise segregation to ensure daughter cells have identical genomic copies. The genus Plasmodium, the causative agent of malaria, displays remarkable aspects of nuclear division throug... Read More about Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation.

Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission (2020)
Journal Article
Le Roch, K. G., Holder, A. A., Bottrill, A. R., Stanway, R. R., Wall, R. J., Tewari, R., …Brady, D. (2020). Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission. Cell Reports, 30(6), 1883-1897.e6. https://doi.org/10.1016/j.celrep.2020.01.033

Condensin is a multi-subunit protein complex regulating chromosome condensation and segregation during cell division. In Plasmodium spp., the causative agent of malaria, cell division is atypical and the role of condensin is unclear. Here we examine... Read More about Plasmodium Condensin Core Subunits SMC2/SMC4 Mediate Atypical Mitosis and Are Essential for Parasite Proliferation and Transmission.

Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission (2019)
Journal Article
Zeeshan, M., Shilliday, F., Liu, T., Abel, S., Mourier, T., Ferguson, D. J. P., …Tewari, R. (2019). Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission. PLoS Pathogens, 15(10), Article e1008048. https://doi.org/10.1371/journal.ppat.1008048

Kinesin-8 proteins are microtubule motors that are often involved in regulation of mitotic spindle length and chromosome alignment. They move towards the plus ends of spindle microtubules and regulate the dynamics of these ends due, at least in some... Read More about Plasmodium kinesin-8X associates with mitotic spindles and is essential for oocyst development during parasite proliferation and transmission.

Kinesin-8B controls basal body function and flagellum formation and is key to malaria transmission (2019)
Journal Article
Zeeshan, M., Ferguson, D. J., Abel, S., Burrrell, A., Rea, E., Brady, D., …Tewari, R. (2019). Kinesin-8B controls basal body function and flagellum formation and is key to malaria transmission. Life Science Alliance, 2(4), 1-13. https://doi.org/10.26508/lsa.201900488

Eukaryotic flagella are conserved microtubule-based organelles that drive cell motility. Plasmodium, the causative agent of malaria, has a single flagellate stage: the male gamete in the mosquito. Three rounds of endomitotic division in male gametocy... Read More about Kinesin-8B controls basal body function and flagellum formation and is key to malaria transmission.

Systematic analysis of Plasmodium myosins reveals differential expression, localisation, and function in invasive and proliferative parasite stages (2019)
Journal Article
Wall, R. J., Zeeshan, M., Katris, N. J., Limenitakis, R., Rea, E., Stock, J., …Tewari, R. (2019). Systematic analysis of Plasmodium myosins reveals differential expression, localisation, and function in invasive and proliferative parasite stages. Cellular Microbiology, 21(10), Article e13082. https://doi.org/10.1111/cmi.13082

The myosin superfamily comprises of actin‐dependent eukaryotic molecular motors important in a variety of cellular functions. Although well studied in many systems, knowledge of their functions in Plasmodium, the causative agent of malaria, is restri... Read More about Systematic analysis of Plasmodium myosins reveals differential expression, localisation, and function in invasive and proliferative parasite stages.

Plasmodium centrin PbCEN-4 localizes to the putative MTOC and is dispensable for malaria parasite proliferation (2018)
Journal Article
Roques, M., Stanway, R. R., Rea, E. I., Markus, R., Brady, D., Holder, A. A., …Tewari, R. (2018). Plasmodium centrin PbCEN-4 localizes to the putative MTOC and is dispensable for malaria parasite proliferation. Biology Open, 8(1), Article bio.036822. https://doi.org/10.1242/bio.036822

Centrins are calmodulin-like phosphoproteins present in the centrosome and play an active role in the duplication, separation and organization of centrosomal structures such as the microtubule-organizing centre (MTOC) during mitosis. They are also ma... Read More about Plasmodium centrin PbCEN-4 localizes to the putative MTOC and is dispensable for malaria parasite proliferation.

Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis (2018)
Journal Article
Wall, R. J., Ferguson, D. J. P., Freville, A., Franke-Fayard, B., Brady, D., Zeeshan, M., …Tewari, R. (in press). Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis. Scientific Reports, 8(1), Article 5610. https://doi.org/10.1038/s41598-018-23871-9

The anaphase promoting complex/cyclosome (APC/C) is a highly conserved multi-subunit E3 ubiquitin ligase that controls mitotic division in eukaryotic cells by tagging cell cycle regulators for proteolysis. APC3 is a key component that contributes to... Read More about Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis.

Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development (2017)
Journal Article
Saini, E., Zeeshan, M., Brady, D., Pandey, R., Kaiser, G., Koreny, L., …Tewari, R. (in press). Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development. Scientific Reports, 7(1), Article 15577. https://doi.org/10.1038/s41598-017-15781-z

Plasmodium parasites, the causative agents of malaria, possess a distinctive membranous structure of flattened alveolar vesicles supported by a proteinaceous network, and referred to as the inner membrane complex (IMC). The IMC has a role in actomyos... Read More about Photosensitized INA-Labelled protein 1 (PhIL1) is novel component of the inner membrane complex and is required for Plasmodium parasite development.

SAS6-like protein in Plasmodium indicates that conoid-associated apical complex proteins persist in invasive stages within the mosquito vector (2016)
Journal Article
Wall, R. J., Roques, M., Katris, N. J., Koreny, L., Stanway, R. R., Brady, D., …Tewari, R. (2016). SAS6-like protein in Plasmodium indicates that conoid-associated apical complex proteins persist in invasive stages within the mosquito vector. Scientific Reports, 6(1), https://doi.org/10.1038/srep28604

The SAS6-like (SAS6L) protein, a truncated paralogue of the ubiquitous basal body/centriole protein SAS6, has been characterised recently as a flagellum protein in trypanosomatids, but associated with the conoid in apicomplexan Toxoplasma. The conoid... Read More about SAS6-like protein in Plasmodium indicates that conoid-associated apical complex proteins persist in invasive stages within the mosquito vector.

Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes (2015)
Journal Article
Roques, M., Wall, R. J., Douglass, A. P., Ramaprasad, A., Ferguson, D. J. P., Kaindama, M. L., …Tewari, R. (2015). Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes. PLoS Pathogens, 11(11), Article e1005273. https://doi.org/10.1371/journal.ppat.1005273

Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisi... Read More about Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes.

Genome-wide Functional Analysis of Plasmodium Protein Phosphatases Reveals Key Regulators of Parasite Development and Differentiation (2014)
Journal Article
Guttery, D. S., TEWARI, R., Poulin, B., Ramaprasad, A., Wall, R. J., Ferguson, D. J., …Pain, A. (2014). Genome-wide Functional Analysis of Plasmodium Protein Phosphatases Reveals Key Regulators of Parasite Development and Differentiation. Cell Host and Microbe, 16(1), 128-140. https://doi.org/10.1016/j.chom.2014.05.020

Reversible protein phosphorylation regulated by kinases and phosphatases controls many cellular processes. Although essential functions for the malaria parasite kinome have been reported, the roles of most protein phosphatases (PPs) during Plasmodium... Read More about Genome-wide Functional Analysis of Plasmodium Protein Phosphatases Reveals Key Regulators of Parasite Development and Differentiation.

Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach (2013)
Journal Article
Wright, M., Clough, B., Rackham, M., Rangachari, K., Brannigan, J., Grainger, M., …Tate, E. W. (2014). Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach. Nature Chemistry, 6(2), 112-121. https://doi.org/10.1038/NCHEM.1830

Malaria is an infectious disease caused by parasites of the genus Plasmodium, which leads to approximately one million deaths per annum worldwide. Chemical validation of new antimalarial targets is urgently required in view of rising resistance to cu... Read More about Validation of N-myristoyltransferase as an antimalarial drug target using an integrated chemical biology approach.

A unique protein phosphatase with kelch-like domains (PPKL) in plasmodium modulates ookinete differentiation, motility and invasion (2012)
Journal Article
Guttery, D. S., Poulin, B., Ferguson, D. J. P., Szöőr, B., Wickstead, B., Carroll, P. L., …Tewari, R. (2012). A unique protein phosphatase with kelch-like domains (PPKL) in plasmodium modulates ookinete differentiation, motility and invasion. PLoS Pathogens, 8(9), Article e1002948. https://doi.org/10.1371/journal.ppat.1002948

Protein phosphorylation and dephosphorylation (catalysed by kinases and phosphatases, respectively) are post-translational modifications that play key roles in many eukaryotic signalling pathways, and are often deregulated in a number of pathological... Read More about A unique protein phosphatase with kelch-like domains (PPKL) in plasmodium modulates ookinete differentiation, motility and invasion.

A putative homologue of CDC20/CDH1 in the malaria parasite is essential for male gamete development (2012)
Journal Article
Guttery, D. S., Ferguson, D. J. P., Poulin, B., Xu, Z., Straschil, U., Klop, O., …Tewari, R. (2012). A putative homologue of CDC20/CDH1 in the malaria parasite is essential for male gamete development. PLoS Pathogens, 8(2), Article e1002554. https://doi.org/10.1371/journal.ppat.1002554

Cell-cycle progression is governed by a series of essential regulatory proteins. Two major regulators are cell-division cycle protein 20 (CDC20) and its homologue, CDC20 homologue 1 (CDH1), which activate the anaphase-promoting complex/cyclosome (APC... Read More about A putative homologue of CDC20/CDH1 in the malaria parasite is essential for male gamete development.

Effects of ?-synuclein overexpression in transgenic Caenorhabditis elegans strains (2012)
Journal Article
Bodhicharla, R., Nagarajan, A., Winter, J., Adenle, A., Nazir, A., Brady, D., …de Pomerai, D. I. (2012). Effects of α-synuclein overexpression in transgenic Caenorhabditis elegans strains. CNS and Neurological Disorders - Drug Targets, 11(8), https://doi.org/10.2174/1871527311211080005

The neural protein α-synuclein aggregates both in vivo and in vitro to form insoluble fibrils that are involved in Parkinson’s disease pathogenesis. We have generated α-synuclein/fluorescent-protein fusion constructs overexpressed in muscle cells of... Read More about Effects of ?-synuclein overexpression in transgenic Caenorhabditis elegans strains.