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Measurement of the total angiotensinogen and its reduced and oxidised forms in human plasma using targeted LC-MS/MS (2018)
Journal Article
Dahabiyeh, L. A., Tooth, D., Carrell, R. W., Read, R. J., Yan, Y., Pipkin, F. B., & Barrett, D. A. (2018). Measurement of the total angiotensinogen and its reduced and oxidised forms in human plasma using targeted LC-MS/MS. Analytical and Bioanalytical Chemistry, doi:10.1007/s00216-018-1455-2

Angiotensinogen (AGT) is a critical protein in the renin-angiotensin-aldosterone system and may have an important role in the pathogenesis of pre-eclampsia. The disulphide linkage between cysteines 18 and 138 has a key role in the redox switch of AGT... Read More

Human C-reactive protein aggravates osteoarthritis development in mice on a high-fat diet (2018)
Journal Article
Kozijn, A., Tartjiono, M., Ravipati, S., van der Ham, F., Barrett, D., Mastbergen, S., …Stoop, R. (2019). Human C-reactive protein aggravates osteoarthritis development in mice on a high-fat diet. Osteoarthritis and Cartilage, 27(1), 118-128. doi:10.1016/j.joca.2018.09.007

Objective: C-reactive protein (CRP) levels can be elevated in osteoarthritis (OA) patients. In addition to indicating systemic inflammation, it is suggested that CRP itself can play a role in OA development. Obesity and metabolic syndrome are importa... Read More

Omega-6 oxylipins generated by soluble epoxide hydrolase are associated with knee osteoarthritis (2018)
Journal Article
Valdes, A. M., Ravipati, S., Pousinis, P., Menni, C., Mangino, M., Abhishek, A., …Doherty, M. (in press). Omega-6 oxylipins generated by soluble epoxide hydrolase are associated with knee osteoarthritis. Journal of Lipid Research, 59(7), doi:10.1194/jlr.P085118. ISSN 0022-2275

Omega-6 fatty acids are important inflammatory mediators increased in joints with osteoarthritis (OA) joints. To investigate the relationship between omega-6 fatty acids and knee OA 22 omega-6 lipids (arachidonic acid, linoleic acid and 20 oxylipins)... Read More

Sustenance and performance: nutritional reserves, longevity and contest outcomes of fed and starved adult parasitoid wasps (2018)
Journal Article
Snart, C. J., Kapranas, A., Williams, H., Barrett, D. A., & Hardy, I. C. (2018). Sustenance and performance: nutritional reserves, longevity and contest outcomes of fed and starved adult parasitoid wasps. Frontiers in Ecology and Evolution, doi:10.3389/fevo.2018.00012

Dyadic contests for possession of resources occur across a wide range of animal taxa, with contest outcome often being heavily influenced by the energetic reserves of the competitors. The majority of studied parasitoid wasps lack de novo lipogenesis,... Read More

The endocannabinoid, anandamide, induces cannabinoid receptor-independent cell death in renal proximal tubule cells (2017)
Journal Article
Schlosser, M., Löser, H., Siegmund, S. V., Montesinos-Rongen, M., Bindila, L., Lutz, B., …Fries, J. W. (2017). The endocannabinoid, anandamide, induces cannabinoid receptor-independent cell death in renal proximal tubule cells. CellBio, 6(4), doi:10.4236/cellbio.2017.64004. ISSN 2325-7776

Background: The endocannabinoid (EC) system is well characterized in the central nervous system but scarcely studied in peripheral organs. In this paper, we newly identify the effect of the EC anandamide (AEA) upon renal proximal tubule cells. Meth... Read More

Age dependent plasticity in endocannabinoid modulation of pain processing through postnatal development (2017)
Journal Article
H-T. Kwok, C., Devonshire, I. M., Imraish, A., Greenspon, C. M., Lockwood, S., Fielden, C., …Hathway, G. J. (2017). Age dependent plasticity in endocannabinoid modulation of pain processing through postnatal development. PAIN, 158(11), doi:10.1097/j.pain.0000000000001027. ISSN 0304-3959

Significant age and experience-dependent remodelling of spinal and supraspinal neural networks occur resulting in altered pain responses in early life. In adults endogenous opioid peptide and endocannabinoid (ECs) pain control systems exist which mod... Read More

A methodology for elucidating regulatory mechanisms leading to changes in lipid profiles (2017)
Journal Article
Casbas-Pinto, F., Ravipati, S., Barrett, D. A., & Hodgman, C. (in press). A methodology for elucidating regulatory mechanisms leading to changes in lipid profiles. Metabolomics, 13, doi:10.1007/s11306-017-1214-y. ISSN 1573-3890

Introduction It is difficult to elucidate the metabolic and regulatory factors causing lipidome perturbations. Objectives This work simplifies this process. Methods A method has been developed to query an online holistic lipid metabolic netwo... Read More

Repurposed drugs targeting eIF2α-P-mediated translational repression prevent neurodegeneration in mice (2017)
Journal Article
Halliday, M., Radford, H., Zents, K. A., Molloy, C., Moreno, J. A., Verity, N. C., …Mallucci, G. R. (2017). Repurposed drugs targeting eIF2α-P-mediated translational repression prevent neurodegeneration in mice. Brain, 140(6), 1768-1783. doi:10.1093/brain/awx074

Signalling through the PERK/eIF2α-P branch of the unfolded protein response plays a critical role in controlling protein synthesis rates in cells. This pathway is overactivated in brains of patients with Alzheimer’s disease and related disorders and... Read More

The role of CB1 in intestinal permeability and inflammation (2017)
Journal Article
Karwad, M. A., Couch, D. G., Theophilidou, E., Sarmad, S., Barrett, D. A., Larvin, M., …O'Sullivan, S. (in press). The role of CB1 in intestinal permeability and inflammation. FASEB Journal, 31(8), doi:10.1096/fj.201601346R. ISSN 0892-6638

The endocannabinoid system has previously been shown to play a role in the permeability and inflammatory response of the human gut. The goal of our study was to determine the effects of endogenous anandamide (AEA) and 2-arachidonoyl glycerol (2-AG) o... Read More

Mouse mitochondrial lipid composition is defined by age in brain and muscle (2017)
Journal Article
Pollard, A. K., Ortori, C. A., Stöger, R., Barrett, D. A., & Chakrabarti, L. (in press). Mouse mitochondrial lipid composition is defined by age in brain and muscle. Aging, 9(3), doi:10.18632/aging.101204. ISSN 1945-4589

Functionality of the lipid rich mitochondrial organelle declines with increased age. Recent advances in lipidomic technologies allowed us to perform a global characterisation of lipid composition in two different tissue types and age ranges. Ultra-hi... Read More