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All Outputs (34)

Low-Power Sonication Can Alter Extracellular Vesicle Size and Properties (2021)
Journal Article
Nizamudeen, Z. A., Xerri, R., Parmenter, C., Suain, K., Markus, R., Chakrabarti, L., & Sottile, V. (2021). Low-Power Sonication Can Alter Extracellular Vesicle Size and Properties. Cells, 10(9), Article 2413. https://doi.org/10.3390/cells10092413

Low-power sonication is widely used to disaggregate extracellular vesicles (EVs) after isolation, however, the effects of sonication on EV samples beyond dispersion are unclear. The present study analysed the characteristics of EVs collected from mes... Read More about Low-Power Sonication Can Alter Extracellular Vesicle Size and Properties.

New models of adipogenic differentiation highlight a cell-autonomous response to temperature (2020)
Journal Article
Velickovic, K., Lugo Leija, H., McGinlay, S., Symonds, M., & Sottile, V. (2020). New models of adipogenic differentiation highlight a cell-autonomous response to temperature. Biocell, 44(4), 501-512. https://doi.org/10.32604/biocell.2020.012942

Temperature is a key regulator of brown adipose tissue (BAT) function, acting through central sensory inputs to influence metabolism and energy storage. Although animal models have produced a wealth of information on the pathways, effectors and respo... Read More about New models of adipogenic differentiation highlight a cell-autonomous response to temperature.

Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells (2020)
Journal Article
Pérez-Cota, F., Fuentes-Domínguez, R., La Cavera III, S., Hardiman, W., Yao, M., Setchfield, K., …Clark, M. (2020). Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells. Journal of Applied Physics, 128(16), Article 160902. https://doi.org/10.1063/5.0023744

© 2020 Author(s). Characterization of the elasticity of biological cells is growing as a new way to gain insight into cell biology. Cell mechanics are related to most aspects of cellular behavior, and applications in research and medicine are broad.... Read More about Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells.

Targeting glutamine synthesis inhibits stem cell adipogenesis in vitro (2020)
Journal Article
Velickovic, K., Lugo Leija, H. A., Surrat, A., Kim, D., Sacks, H., Symonds, M. E., & Sottile, V. (2020). Targeting glutamine synthesis inhibits stem cell adipogenesis in vitro. Cellular Physiology and Biochemistry, 54(5), 917-927. https://doi.org/10.33594/000000278

Background/Aims: Glutamine is the most abundant amino acid in the body and has a metabolic role as a precursor for protein, amino sugar and nucleotide synthesis. After glucose, glutamine is the main source of energy in cells and has recently been sho... Read More about Targeting glutamine synthesis inhibits stem cell adipogenesis in vitro.

Cold-induced beigeing of stem cell-derived adipocytes is not fully reversible after return to normothermia (2020)
Journal Article
Lugo Leija, H. A., Velickovic, K., Bloor, I., Sacks, H., Symonds, M. E., & Sottile, V. (2020). Cold-induced beigeing of stem cell-derived adipocytes is not fully reversible after return to normothermia. Journal of Cellular and Molecular Medicine, 24(19), 11434-11444. https://doi.org/10.1111/jcmm.15749

Beige adipocytes possess the morphological and biochemical characteristics of brown adipocytes, including the mitochondrial uncoupling protein (UCP)1. Mesenchymal stem cells (MSCs) are somatic multipotent progenitors which differentiate into lipid-la... Read More about Cold-induced beigeing of stem cell-derived adipocytes is not fully reversible after return to normothermia.

Impact of Serum Source on Human Mesenchymal Stem Cell Osteogenic Differentiation in Culture (2019)
Journal Article
Popov, A., Scotchford, C., Grant, D., & Sottile, V. (2019). Impact of Serum Source on Human Mesenchymal Stem Cell Osteogenic Differentiation in Culture. International Journal of Molecular Sciences, 20(20), https://doi.org/10.3390/ijms20205051

Human mesenchymal stem cells (MSCs) show promise for musculoskeletal repair applications. Animal-derived serum is extensively used for MSC culture as a source of nutrients, extracellular matrix proteins and growth factors. However, the routine use of... Read More about Impact of Serum Source on Human Mesenchymal Stem Cell Osteogenic Differentiation in Culture.

Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells (2019)
Journal Article
De Melo, N., McGinlay, S., Markus, R., Macri-Pellizzeri, L., Symonds, M. E., Ahmed, I., & Sottile, V. (2019). Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells. Biomimetics, 4(3), 1-10. https://doi.org/10.3390/biomimetics4030048

Mesenchymal stem cells (MSCs) are progenitors for bone-forming osteoblasts and lipid-storing adipocytes, two major lineages co-existing in bone marrow. When isolated in vitro, these stem cells recapitulate osteoblast or adipocyte formation if treated... Read More about Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells.

Caffeine exposure induces browning features in adipose tissue in vitro and in vivo (2019)
Journal Article
Velickovic, K., Wayne, D., Lugo Leija, H. A., Bloor, I., Morris, D. E., Law, J., …Sottile, V. (2019). Caffeine exposure induces browning features in adipose tissue in vitro and in vivo. Scientific Reports, 9(1), Article 9104. https://doi.org/10.1038/s41598-019-45540-1

Brown adipose tissue (BAT) is able to rapidly generate heat and metabolise macronutrients, such as glucose and lipids, through activation of mitochondrial uncoupling protein 1 (UCP1). Diet can modulate UCP1 function but the capacity of individual nut... Read More about Caffeine exposure induces browning features in adipose tissue in vitro and in vivo.

Porous phosphate-based glass microspheres show biocompatibility, tissue infiltration and osteogenic onset in an ovine bone defect model (2019)
Journal Article
McLaren, J. S., Macri-Pellizzeri, L., Zakir Hossain, K. M., Patel, U., Grant, D. M., Scammell, B. E., …Sottile, V. (2019). Porous phosphate-based glass microspheres show biocompatibility, tissue infiltration and osteogenic onset in an ovine bone defect model. ACS Applied Materials and Interfaces, 11(17), 15436–15446. https://doi.org/10.1021/acsami.9b04603

Phosphate-based glasses (PBG) are bioactive and fully degradable materials with tailorable degradation rates. PBGs can be produced as porous microspheres through a single-step process, using changes in their formulation and geometry to produce varyin... Read More about Porous phosphate-based glass microspheres show biocompatibility, tissue infiltration and osteogenic onset in an ovine bone defect model.

Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration (2019)
Journal Article
Harrison, R., Luckett, J., Marsh, S., Lugo Leija, H. A., Salih, S., Alkharji, R., & Sottile, V. (2019). Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration. Molecules, 24(8), Article 1563. https://doi.org/10.3390/molecules24081563

The success of cell therapy approaches is greatly dependent on the ability to precisely deliver and monitor transplanted stem cell grafts at treated sites. Iron oxide particles, traditionally used in vivo for magnetic resonance imaging (MRI), have be... Read More about Magnetically Assisted Control of Stem Cells Applied in 2D, 3D and In Situ Models of Cell Migration.

Integrated multi-assay culture model for stem cell chondrogenic differentiation (2019)
Journal Article
Prosser, A., Scotchford, C., Roberts, G., Grant, D., & Sottile, V. (2019). Integrated multi-assay culture model for stem cell chondrogenic differentiation. International Journal of Molecular Sciences, 20(4), https://doi.org/10.3390/ijms20040951

Recent osteochondral repair strategies highlight the promise of mesenchymal progenitors, an accessible stem cell source with osteogenic and chondrogenic potential, used in conjunction with biomaterials for tissue engineering. For this, regenerative m... Read More about Integrated multi-assay culture model for stem cell chondrogenic differentiation.

Intracellular processing of silica-coated superparamagnetic iron nanoparticles in human mesenchymal stem cells (2019)
Journal Article
Harrison, R. P., Chauhan, V. M., Onion, D., Aylott, J. W., & Sottile, V. (2019). Intracellular processing of silica-coated superparamagnetic iron nanoparticles in human mesenchymal stem cells. RSC Advances, 9(6), 3176-3184. https://doi.org/10.1039/C8RA09089K

Silica-coated Superparamagnetic Iron Nanoparticles (SiMAGs) are an exciting biomedical technology capable of targeted delivery of cell-based therapeutics and disease diagnosis. However, in order to realise their full clinical potential, their intrace... Read More about Intracellular processing of silica-coated superparamagnetic iron nanoparticles in human mesenchymal stem cells.

In vitro cellular testing of Strontium/Calcium substituted phosphate glass discs and microspheres shows potential for bone regeneration (2019)
Journal Article
Patel, U., Macri-Pellizzeri, L., Hossain, K. M. Z., Scammell, B. E., Grant, D. M., Scotchford, C. A., …Sottile, V. (2019). In vitro cellular testing of Strontium/Calcium substituted phosphate glass discs and microspheres shows potential for bone regeneration. Journal of Tissue Engineering and Regenerative Medicine, 13(3), 396-405. https://doi.org/10.1002/term.2796

Phosphate glasses (PBG) are ideal materials for regenerative medicine strategies since their composition, degradation rates and ion release profiles can easily be controlled. Strontium has previously been found to simultaneously affect bone resorptio... Read More about In vitro cellular testing of Strontium/Calcium substituted phosphate glass discs and microspheres shows potential for bone regeneration.

Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging (2018)
Journal Article
Nizamudeen, Z., Markus, R., Lodge, R., Parmenter, C., Platt, M., Chakrabarti, L., & Sottile, V. (2018). Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1865(12), 1891-1900. https://doi.org/10.1016/j.bbamcr.2018.09.008

Extracellular vesicles (EVs) have prevalent roles in cancer biology and regenerative medicine. Conventional techniques for characterising EVs including electron microscopy (EM), nanoparticle tracking analysis (NTA) and tuneable resistive pulse sensin... Read More about Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging.

Performance of multiphase scaffolds for bone repair based on two-photon polymerized poly(d,l-lactide-co-?-caprolactone), recombinamers hydrogel and nano-HA (2018)
Journal Article
Felfel, R. M., Gupta, D., Zabidi, A. Z., Prosser, A., Scotchford, C. A., Sottile, V., & Grant, D. M. (2018). Performance of multiphase scaffolds for bone repair based on two-photon polymerized poly(d,l-lactide-co-?-caprolactone), recombinamers hydrogel and nano-HA. Materials and Design, 160, 455-467. https://doi.org/10.1016/j.matdes.2018.09.035

Multiphase hybrids were fabricated from poly(d,l-lactide-co-ɛ-caprolactone) (PLCL) copolymer scaffolds impregnated with silk-elastin-like recombinamers (SELRs) hydrogel containing 2 wt% hydroxyapatite nanoparticles (nHA). The PLCL scaffolds, triply-p... Read More about Performance of multiphase scaffolds for bone repair based on two-photon polymerized poly(d,l-lactide-co-?-caprolactone), recombinamers hydrogel and nano-HA.

Porous calcium phosphate glass microspheres for orthobiologic applications (2018)
Journal Article
Hossain, K. M. Z., Patel, U., Kennedy, A. R., Macri-Pellizzeri, L., Sottile, V., Grant, D. M., …Ahmed, I. (2018). Porous calcium phosphate glass microspheres for orthobiologic applications. Acta Biomaterialia, 72, 396-406. https://doi.org/10.1016/j.actbio.2018.03.040

© 2018 Orthobiologics is a rapidly advancing field utilising cell-based therapies and biomaterials to enable the body to repair and regenerate musculoskeletal tissues. This paper reports on a cost-effective flame spheroidisation process for producti... Read More about Porous calcium phosphate glass microspheres for orthobiologic applications.

Low temperature exposure induces browning of bone marrow stem cell derived adipocytes in vitro (2018)
Journal Article
Velickovic, K., Lugo-Leija, H. A., Bloor, I., Law, J., Sacks, H., Symonds, M. E., & Sottile, V. (2018). Low temperature exposure induces browning of bone marrow stem cell derived adipocytes in vitro. Scientific Reports, 8, Article 4974. https://doi.org/10.1038/s41598-018-23267-9

Brown and beige adipocytes are characterised as expressing the unique mitochondrial uncoupling protein (UCP)1 for which the primary stimulus in vivo is cold exposure. The extent to which cold-induced UCP1 activation can also be achieved in vitro, and... Read More about Low temperature exposure induces browning of bone marrow stem cell derived adipocytes in vitro.

Live quantitative monitoring of mineral deposition in stem cells using tetracycline hydrochloride (2018)
Journal Article
Macri-Pellizzeri, L., De Melo, N., Ahmed, I., Grant, D. M., Scammell, B., & Sottile, V. (2018). Live quantitative monitoring of mineral deposition in stem cells using tetracycline hydrochloride. Tissue Engineering Part C Methods, 24(3), https://doi.org/10.1089/ten.TEC.2017.0400

The final stage of in vitro osteogenic differentiation is characterized by the production of mineral deposits containing calcium cations and inorganic phosphates, which populate the extracellular matrix surrounding the cell monolayer. Conventional hi... Read More about Live quantitative monitoring of mineral deposition in stem cells using tetracycline hydrochloride.

Exposure to the ROCK inhibitor fasudil promotes gliogenesis of neural stem cells in vitro (2018)
Journal Article
Nizamudeen, Z. A., Chakrabarti, L., & Sottile, V. (2018). Exposure to the ROCK inhibitor fasudil promotes gliogenesis of neural stem cells in vitro. Stem Cell Research, 28, 75-86. https://doi.org/10.1016/j.scr.2018.02.001

Fasudil is a clinically approved Rho-associated protein kinase (ROCK) inhibitor that has been used widely to treat cerebral consequences of subarachnoid haemorrhage. It is known to have a positive effect on animal models of neurological disorders inc... Read More about Exposure to the ROCK inhibitor fasudil promotes gliogenesis of neural stem cells in vitro.

Role of geometrical cues in bone marrow-derived mesenchymal stem cell survival, growth and osteogenic differentiation (2017)
Journal Article
Gupta, D., Grant, D. M., Hossain, K. M. Z., Ahmed, I., & Sottile, V. (2018). Role of geometrical cues in bone marrow-derived mesenchymal stem cell survival, growth and osteogenic differentiation. Journal of Biomaterials Applications, 32(7), https://doi.org/10.1177/0885328217745699

Mesenchymal stem cells play a vital role in bone formation process by differentiating into osteoblasts, in a tissue that offers not a flat but a discontinuous three-dimensional (3D) topography in vivo. In order to understand how geometry may be affec... Read More about Role of geometrical cues in bone marrow-derived mesenchymal stem cell survival, growth and osteogenic differentiation.