Research Repository

See what's under the surface


Molecular recognition of lipopolysaccaride by the lantibiotic nisin (2018)
Journal Article
Lanne, A. B., Goode, A., Prattley, C., Kumari, D., Drasbek, M. R., Williams, P., …Bonev, B. B. (2019). Molecular recognition of lipopolysaccaride by the lantibiotic nisin. BBA - Biomembranes, 1861(1), 83-92. doi:10.1016/j.bbamem.2018.10.006

Nisin is a lanthionine antimicrobial effective against diverse Gram-positive bacteria and is used as a food preservative worldwide. Its action is mediated by pyrophosphate recognition of the bacterial cell wall receptors lipid II and undecaprenyl pyr... Read More

To boil an egg: substrate binding affects critical stability in thermal unfolding of proteins (2018)
Journal Article
Hussain, R., Hughes, C. S., Jávorfi, T., Siligardi, G., Williams, P., & Bonev, B. B. (2018). To boil an egg: substrate binding affects critical stability in thermal unfolding of proteins. Journal of Physical Chemistry B, 122(8), doi:10.1021/acs.jpcb.7b10643

Thermal unfolding of proteins is used extensively in screening of drug candidates because molecular interactions with ligands and substrates affect strongly protein stability, transition temperature, and cooperativity. We use synchrotron radiation ci... Read More

Recognition of membrane sterols by polyene antifungals amphotericin B and natamycin, a 13C MAS NMR Study (2016)
Journal Article
Ciesielski, F., Griffin, D. C., Loraine, J., Rittig, M., Delves-Broughton, J., & Bonev, B. B. (in press). Recognition of membrane sterols by polyene antifungals amphotericin B and natamycin, a 13C MAS NMR Study. Frontiers in Cell and Developmental Biology, 4, doi:10.3389/fcell.2016.00057. ISSN 2296-634X

The molecular action of polyene macrolides with antifungal activity, amphotericin B and natamycin, involves recognition of sterols in membranes. Physicochemical and functional studies have contributed details to understanding the interactions between... Read More

Docking and molecular dynamics simulations of the ternary complex nisin2:lipid II (2016)
Journal Article
Mulholland, S., Turpin, E. R., Bonev, B. B., & Hirst, J. (2016). Docking and molecular dynamics simulations of the ternary complex nisin2:lipid II. Scientific Reports, 6(21185), doi:10.1038/srep21185. ISSN 2045-2322

Lanthionine antibiotics are an important class of naturally-occurring antimicrobial peptides. The best-known, nisin, is a commercial food preservative. However, structural and mechanistic details on nisin/lipid II membrane complexes are currently lac... Read More