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The polyadenylation inhibitor cordycepin reduces pain, inflammation and joint pathology in rodent models of osteoarthritis (2019)
Journal Article
Ashraf, S., Radhi, M., Gowler, P., Burston, J. J., Gandhi, R. D., Thorn, G. J., …Chapman, V. (2019). The polyadenylation inhibitor cordycepin reduces pain, inflammation and joint pathology in rodent models of osteoarthritis. Scientific Reports, 9, 1-17. doi:10.1038/s41598-019-41140-1

Clinically, osteoarthritis (OA) pain is significantly associated with synovial inflammation. Identification of the mechanisms driving inflammation could reveal new targets to relieve this prevalent pain state. Herein, a role of polyadenylation in OA... Read More about The polyadenylation inhibitor cordycepin reduces pain, inflammation and joint pathology in rodent models of osteoarthritis.

A complex interplay between the extracellular matrix and the innate immune response to microbial pathogens (2018)
Journal Article
Tomlin, H., & Piccinini, A. M. (2018). A complex interplay between the extracellular matrix and the innate immune response to microbial pathogens. Immunology, 155(2), (186-201). doi:10.1111/imm.12972. ISSN 0019-2805

The role of the host extracellular matrix (ECM) in infection tends to be neglected. However, the complex interactions between invading pathogens, host tissues and immune cells occur in the context of the ECM. On the pathogen side, a variety of surfac... Read More about A complex interplay between the extracellular matrix and the innate immune response to microbial pathogens.

Mesenchymal stem cell-conditioned medium reduces disease severity and immune responses in inflammatory arthritis (2017)
Journal Article
Kay, A. G., Long, G., Tyler, G., Stefan, A., Broadfoot, S. J., Piccinini, A. M., …Kehoe, O. (2017). Mesenchymal stem cell-conditioned medium reduces disease severity and immune responses in inflammatory arthritis. Scientific Reports, 7, doi:10.1038/s41598-017-18144-w

We evaluated the therapeutic potential of mesenchymal stem cell-conditioned medium (CM-MSC) as an alternative to cell therapy in an antigen-induced model of arthritis (AIA). Disease severity and cartilage loss were evaluated by histopathological anal... Read More about Mesenchymal stem cell-conditioned medium reduces disease severity and immune responses in inflammatory arthritis.

Mapping tenascin-C interaction with toll-like receptor 4 reveals a new subset of endogenous inflammatory triggers (2017)
Journal Article
Zuliani-Alvarez, L., Marzeda, A. M., Deligne, C., Schwenzer, A., McCann, F. E., Marsden, B. D., …Midwood, K. S. (in press). Mapping tenascin-C interaction with toll-like receptor 4 reveals a new subset of endogenous inflammatory triggers. Nature Communications, 8(1), doi:10.1038/s41467-017-01718-7

Pattern recognition underpins innate immunity; the accurate identification of danger, including infection, injury, or tumor, is key to an appropriately targeted immune response. Pathogen detection is increasingly well defined mechanistically, but the... Read More about Mapping tenascin-C interaction with toll-like receptor 4 reveals a new subset of endogenous inflammatory triggers.

Screening for novel endogenous inflammatory stimuli using the secreted embryonic alkaline phosphatase NF-κB reporter assay (2017)
Journal Article
Zuliani-Alvarez, L., Piccinini, A. M., & Midwood, K. S. (2017). Screening for novel endogenous inflammatory stimuli using the secreted embryonic alkaline phosphatase NF-κB reporter assay. Bio-protocol, 7(7), doi:10.21769/BioProtoc.2220

An immune response can be activated by pathogenic stimuli, as well as endogenous danger signals, triggering the activation of pattern recognition receptors and initiating signalling cascades that lead to inflammation. This method uses THP1-Blue™ cell... Read More about Screening for novel endogenous inflammatory stimuli using the secreted embryonic alkaline phosphatase NF-κB reporter assay.

Distinct microenvironmental cues stimulate divergent TLR4-mediated signaling pathways in macrophages (2016)
Journal Article
Piccinini, A. M., Zuliani-Alvarez, L., Lim, J. M., & Midwood, K. S. (2016). Distinct microenvironmental cues stimulate divergent TLR4-mediated signaling pathways in macrophages. Science Signaling, 9(443), ra86-ra86. https://doi.org/10.1126/scisignal.aaf3596

Macrophages exhibit a phenotypic plasticity that enables them to orchestrate specific immune responses to distinct threats. However, the factors that control macrophage behaviour in a context dependent manner are not well understood. Lipopolysacchari... Read More about Distinct microenvironmental cues stimulate divergent TLR4-mediated signaling pathways in macrophages.

Interfering with the CCL2–glycosaminoglycan axis as a potential approach to modulate neuroinflammation (2016)
Journal Article
Gschwandtner, M., Piccinini, A. M., Geriza, T., Adage, T., & Kungl, A. (2016). Interfering with the CCL2–glycosaminoglycan axis as a potential approach to modulate neuroinflammation. Neuroscience Letters, 626, doi:10.1016/j.neulet.2016.05.037

Multiple Sclerosis, a chronic inflammatory demyelinating disease of the central nervous system, involves an increased expression of monocyte chemotactic protein 1 MCP1-/CCL2. For exerting its chemotactic effects, chemokine binding to glycosaminoglyca... Read More about Interfering with the CCL2–glycosaminoglycan axis as a potential approach to modulate neuroinflammation.

Investigating the role of toll-like receptors in models of arthritis (2016)
Journal Article
Piccinini, A. M., Williams, L., McCann, F. E., & Midwood, K. S. (2016). Investigating the role of toll-like receptors in models of arthritis. Methods in Molecular Biology, 1390, (351-381). doi:10.1007/978-1-4939-3335-8_22

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent synovial inflammation leading to tissue destruction and progressive loss of joint function. Here we describe two methods that can be used to assess the contribution... Read More about Investigating the role of toll-like receptors in models of arthritis.

Illustrating the interplay between the extracellular matrix and microRNAs (2014)
Journal Article
Piccinini, A. M., & Midwood, K. S. (2014). Illustrating the interplay between the extracellular matrix and microRNAs. International Journal of Experimental Pathology, 95(3), (158-180). doi:10.1111/iep.12079. ISSN 0959-9673

The discovery of cell surface receptors that bind to extracellular matrix (ECM) components marked a new era in biological research. Since then there has been an increasing appreciation of the importance of studying cells in the context of their extra... Read More about Illustrating the interplay between the extracellular matrix and microRNAs.