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Sonic Hedgehog Ligand: A Role in Formation of a Mesenchymal Niche in Human Pancreatic Ductal Adenocarcinoma (2019)
Journal Article
Saini, F., Argent, R. H., & Grabowska, A. (2019). Sonic Hedgehog Ligand: A Role in Formation of a Mesenchymal Niche in Human Pancreatic Ductal Adenocarcinoma. Cells, 8(5), 1-15. https://doi.org/10.3390/cells8050424

Pancreatic ductal adenocarcinoma (PDAC) is characterised by desmoplasia, thought to support progression and chemotherapeutic resistance. The Hedgehog pathway is known to play an important role in this cancer. While the upregulation of Sonic hedgehog... Read More about Sonic Hedgehog Ligand: A Role in Formation of a Mesenchymal Niche in Human Pancreatic Ductal Adenocarcinoma.

Penetration and uptake of Nanoparticles in 3D tumour spheroids (2019)
Journal Article
Tchoryk, A., Taresco, V., Argent, R., Ashford, M. B., Gellert, P., Stolnik, S., …Garnett, M. C. (2019). Penetration and uptake of Nanoparticles in 3D tumour spheroids. Bioconjugate Chemistry, 30(5), 1371-1384. https://doi.org/10.1021/acs.bioconjchem.9b00136

Animal models are effective for assessing tumour localisation of nanosystems, but difficult to use for studying penetration beyond the vasculature. Here, we have used well-characterised HCT116 colorectal cancer spheroids to study the effect of nanopa... Read More about Penetration and uptake of Nanoparticles in 3D tumour spheroids.

Time and cell-dependent effects of endocytosis inhibitors on the internalization of biomolecule markers and nanomaterials (2018)
Journal Article
Sasso, L., Purdie, L., Grabowska, A. M., Jones, A. T., & Alexander, C. (2018). Time and cell-dependent effects of endocytosis inhibitors on the internalization of biomolecule markers and nanomaterials. Journal of Interdisciplinary Nanomedicine, 3(2), https://doi.org/10.1002/jin2.39

Endocytosis is an essential function of cells, with key roles in the internalisation of nutrients, signal molecules and also drugs. Endocytic processes are therefore widely investigated in the context of drug delivery, and inhibitors of endocytic pat... Read More about Time and cell-dependent effects of endocytosis inhibitors on the internalization of biomolecule markers and nanomaterials.

Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA (2018)
Journal Article
Capel, V., Vllasaliu, D., Watts, P., Clarke, P. A., Luxton, D., Grabowska, A. M., …Stolnik, S. (in press). Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA. Drug Delivery, 25(1), https://doi.org/10.1080/10717544.2018.1440668

Despite research efforts full potential of siRNA-based therapeutics has not yet been fully realized due to a need for suitable, effective delivery formulations. Here, we examine a potential of a new class of water-soluble chitosans as siRNA platform... Read More about Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA.

Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells (2018)
Journal Article
Alshehri, A. A., Grabowska, A. M., & Stolnik, S. (in press). Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells. Scientific Reports, 8(1), https://doi.org/10.1038/s41598-018-22166-3

Design of an efficient delivery system is a generally recognised bottleneck in translation of siRNA technology into clinic. Despite research efforts, cellular processes that determine efficiency of siRNA silencing achieved by different delivery formu... Read More about Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells.

A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells (2018)
Journal Article
Ahmed, E. M., Bandopadhyay, G., Coyle, B., & Grabowska, A. M. (2018). A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells. Cellular Oncology, 41(3), https://doi.org/10.1007/s13402-018-0374-8

Purpose Glioblastoma Multiforme (GBM) is the commonest brain tumour in adults. A population of cells, known as cancer stem cells (CSCs), is thought to mediate chemo/radiotherapy resistance. CD133 is a cell surface marker to identify and isolate... Read More about A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells.

Stable DHLA–PEG capped PbS quantum dots: from synthesis to near-infrared biomedical imaging (2018)
Journal Article
Zamberlan, F., Turyanska, L., Patanè, A., Liu, Z., Williams, H., Fay, M., …Grabowska, A. (in press). Stable DHLA–PEG capped PbS quantum dots: from synthesis to near-infrared biomedical imaging. Journal of Materials Chemistry B, 6, https://doi.org/10.1039/c7tb02912h

The short shelf-life of water-soluble quantum dots (QDs) due to colloidal instability represents a major drawback to their exploitation. This work examines the colloidal stability of PbS nanoparticles capped with dihydrolipoic acid–polyethylene glyco... Read More about Stable DHLA–PEG capped PbS quantum dots: from synthesis to near-infrared biomedical imaging.

In vitro tissue microarrays for quick and efficient spheroid characterisation (2017)
Journal Article
Ivanov, D. P., & Grabowska, A. M. (2018). In vitro tissue microarrays for quick and efficient spheroid characterisation. Slas Discovery, 23(2), https://doi.org/10.1177/2472555217740576

Three-dimensional in vitro microphysiological cultures, such as spheroids and organoids, promise increased patient relevance and therapeutic predictivity compared to reductionist cell monolayers. However, high-throughput characterisation techniques f... Read More about In vitro tissue microarrays for quick and efficient spheroid characterisation.

Control of aggregation temperatures in mixed and blended cytocompatible thermoresponsive block co-polymer nanoparticles (2017)
Journal Article
Foralosso, R., Moir, L., Mastrotto, F., Sasso, L., Tchoryk, A., Selo, A., …Alexander, C. (2017). Control of aggregation temperatures in mixed and blended cytocompatible thermoresponsive block co-polymer nanoparticles. Soft Matter, 40(13), https://doi.org/10.1039/c7sm00920h

A small library of thermoresponsive amphiphilic copolymers based on polylactide-block-poly((2-(2-methoxyethoxy)ethyl methacrylate)-co-(oligoethylene glycol methacrylate)) (PLA-b-P(DEGMA)-co-(OEGMA)), was synthesised by copper-mediated controlled radi... Read More about Control of aggregation temperatures in mixed and blended cytocompatible thermoresponsive block co-polymer nanoparticles.

Breast cancer induced nociceptor aberrant growth and collateral sensory axonal branching (2017)
Journal Article
Austin, M., Elliott, L., Nicolaou, N., Grabowska, A. M., & Hulse, R. P. (2017). Breast cancer induced nociceptor aberrant growth and collateral sensory axonal branching. Oncotarget, 8(44), https://doi.org/10.18632/oncotarget.20609

The tumour and neuron interaction has a significant impact upon disease progression and the patients quality of life. In breast cancer patients, it is known that there is an interaction between the tumour microenvironment and the sensory neurons to i... Read More about Breast cancer induced nociceptor aberrant growth and collateral sensory axonal branching.

Multicomponent analysis of the tumour microenvironment reveals low CD8 T cell number, low stromal caveolin-1 and high tenascin-C and their combination as significant prognostic markers in non-small cell lung cancer (2017)
Journal Article
Onion, D., Isherwood, M., Shridhar, N., Xenophontos, M., Craze, M. L., Day, L. J., …Grabowska, A. M. (2018). Multicomponent analysis of the tumour microenvironment reveals low CD8 T cell number, low stromal caveolin-1 and high tenascin-C and their combination as significant prognostic markers in non-small cell lung cancer. Oncotarget, 9(2), 1760-1771. https://doi.org/10.18632/oncotarget.18880

The complex interplay of the tumour microenvironment (TME) and its role in disease progression and response to therapy is poorly understood. The majority of studies to date focus on individual components or molecules within the TME and so lack the po... Read More about Multicomponent analysis of the tumour microenvironment reveals low CD8 T cell number, low stromal caveolin-1 and high tenascin-C and their combination as significant prognostic markers in non-small cell lung cancer.

High-throughput spheroid screens using volume, resazurin reduction and acid phosphatase activity (2017)
Book Chapter
Ivanov, D. P., Grabowska, A. M., & Garnett, M. C. (2018). High-throughput spheroid screens using volume, resazurin reduction and acid phosphatase activity. In D. F. Gilbert, & O. Friedrich (Eds.), Cell Viability Assays (43-59). Humana Press (Springer). https://doi.org/10.1007/978-1-4939-6960-9_4

© 2017, Springer Science+Business Media LLC. Mainstream adoption of physiologically relevant three-dimensional models has been slow in the last 50 years due to long, manual protocols with poor reproducibility, high price, and closed commercial platfo... Read More about High-throughput spheroid screens using volume, resazurin reduction and acid phosphatase activity.

HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells (2017)
Journal Article
Shah, M., Cardenas, R., Wang, B., Persson, J., Mongan, N. P., Grabowska, A. M., & Allegrucci, C. (2017). HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells. Molecular Cancer, 16(38), https://doi.org/10.1186/s12943-017-0605-z

Background: Homeobox genes are master regulators of cell fate during embryonic development and their expression is altered in cancer. By regulating the balance between cell proliferation and differentiation, they maintain homeostasis of normal tissue... Read More about HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells.

Spheroid arrays for high-throughput single-cell analysis of spatial patterns and biomarker expression in 3D (2017)
Journal Article
Ivanov, D. P., & Grabowska, A. M. (2017). Spheroid arrays for high-throughput single-cell analysis of spatial patterns and biomarker expression in 3D. Scientific Reports, 7(1), Article 41160. https://doi.org/10.1038/srep41160

We describe and share a device, methodology and image analysis algorithms, which allow up to 66 spheroids to be arranged into a gel-based array directly from a culture plate for downstream processing and analysis. Compared to processing individual sa... Read More about Spheroid arrays for high-throughput single-cell analysis of spatial patterns and biomarker expression in 3D.

Separating chemotherapy-related developmental neurotoxicity from cytotoxicity in monolayer and neurosphere cultures of human fetal brain cells (2016)
Journal Article
Ivanov, D. P., Al-Rubai, A., Grabowska, A. M., & Pratten, M. K. (2016). Separating chemotherapy-related developmental neurotoxicity from cytotoxicity in monolayer and neurosphere cultures of human fetal brain cells. Toxicology in Vitro, 37, 88-96. https://doi.org/10.1016/j.tiv.2016.09.007

Chemotherapy-induced neurotoxicity can reduce the quality of life of patients by affecting their intelligence, senses and mobility. Ten percent of safety-related late-stage clinical failures are due to neurological side effects. Animal models are poo... Read More about Separating chemotherapy-related developmental neurotoxicity from cytotoxicity in monolayer and neurosphere cultures of human fetal brain cells.

Mutational signatures in esophageal adenocarcinoma define etiologically distinct subgroups with therapeutic relevance (2016)
Journal Article
Secrier, M., Li, X., de Silva, N., Eldridge, M. D., Contino, G., Bornschein, J., …Grimmond, S. M. (2016). Mutational signatures in esophageal adenocarcinoma define etiologically distinct subgroups with therapeutic relevance. Nature Genetics, 48(10), https://doi.org/10.1038/ng.3659

Esophageal adenocarcinoma (EAC) has a poor outcome, and targeted therapy trials have thus far been disappointing owing to a lack of robust stratification methods. Whole-genome sequencing (WGS) analysis of 129 cases demonstrated that this is a heterog... Read More about Mutational signatures in esophageal adenocarcinoma define etiologically distinct subgroups with therapeutic relevance.

3-dimensional patient-derived lung cancer assays reveal resistance to standards-of-care promoted by stromal cells but sensitivity to histone deacetylase inhibitors (2016)
Journal Article
Onion, D., Argent, R. H., Reece-Smith, A. M., Craze, M. L., Pineda, R. G., Clarke, P. A., …Grabowska, A. M. (2016). 3-dimensional patient-derived lung cancer assays reveal resistance to standards-of-care promoted by stromal cells but sensitivity to histone deacetylase inhibitors. Molecular Cancer Therapeutics, 15(4), https://doi.org/10.1158/1535-7163.MCT-15-0598

There is a growing recognition that current preclinical models do not reflect the tumor microenvironment in cellular, biological, and biophysical content and this may have a profound effect on drug efficacy testing, especially in the era of molecular... Read More about 3-dimensional patient-derived lung cancer assays reveal resistance to standards-of-care promoted by stromal cells but sensitivity to histone deacetylase inhibitors.

MicroRNA in prostate cancer: functional importance and potential as circulating biomarkers (2014)
Journal Article
Jackson, B. L., Grabowska, A., & Ratan, H. L. (2014). MicroRNA in prostate cancer: functional importance and potential as circulating biomarkers. BMC Cancer, 14(1), 1-10. https://doi.org/10.1186/1471-2407-14-930

Background This non-systematic review article aims to summarise the progress made in understanding the functional consequences of microRNA (miRNA) dysregulation in prostate cancer development, and the identification of potential miRNA targets as ser... Read More about MicroRNA in prostate cancer: functional importance and potential as circulating biomarkers.

Targeting human apurinic/apyrimidinic endonuclease 1 (APE1) in phosphatase and tensin homolog (PTEN) deficient melanoma cells for personalized therapy (2014)
Journal Article
Abbotts, R., Jewell, R., Nsengimana, J., Maloney, D. J., Simeonov, A., Seedhouse, C., …Madhusudan, S. (2014). Targeting human apurinic/apyrimidinic endonuclease 1 (APE1) in phosphatase and tensin homolog (PTEN) deficient melanoma cells for personalized therapy. Oncotarget, 5(10), 3273-3286. https://doi.org/10.18632/oncotarget.1926

Phosphatase and tensin homolog (PTEN) loss is associated with genomic instability. APE1 is a key player in DNA base excision repair (BER) and an emerging drug target in cancer. We have developed small molecule inhibitors against APE1 repair nuclease... Read More about Targeting human apurinic/apyrimidinic endonuclease 1 (APE1) in phosphatase and tensin homolog (PTEN) deficient melanoma cells for personalized therapy.

Tagged polymerase chain reaction subtractive hybridization for the enrichment of phage display random peptide libraries (2005)
Journal Article
Tarr, A. W., Boneham, S. P., Grabowska, A. M., & Ball, J. K. (2005). Tagged polymerase chain reaction subtractive hybridization for the enrichment of phage display random peptide libraries. Analytical Biochemistry, 339(1), 61-68. https://doi.org/10.1016/j.ab.2004.12.020

Affinity selection of phage display peptide libraries is routinely used for isolating peptides capable of binding a range of molecules, including antibodies and receptors. This process is most successful when the selecting molecule is relatively pure... Read More about Tagged polymerase chain reaction subtractive hybridization for the enrichment of phage display random peptide libraries.