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Temporal refinement of 3D CNN semantic segmentations on 4D time-series of undersampled tomograms using hidden Markov models (2021)
Journal Article
Bellos, D., Basham, M., Pridmore, T., & French, A. P. (2021). Temporal refinement of 3D CNN semantic segmentations on 4D time-series of undersampled tomograms using hidden Markov models. Scientific Reports, 11(1), Article 23279. https://doi.org/10.1038/s41598-021-02466-x

Recently, several convolutional neural networks have been proposed not only for 2D images, but also for 3D and 4D volume segmentation. Nevertheless, due to the large data size of the latter, acquiring a sufficient amount of training annotations is mu... Read More about Temporal refinement of 3D CNN semantic segmentations on 4D time-series of undersampled tomograms using hidden Markov models.

Towards infield, live plant phenotyping using a reduced-parameter CNN (2019)
Journal Article
Atanbori, J., French, A. P., & Pridmore, T. P. (2020). Towards infield, live plant phenotyping using a reduced-parameter CNN. Machine Vision and Applications, 31, Article 2. https://doi.org/10.1007/s00138-019-01051-7

There is an increase in consumption of agricultural produce as a result of the rapidly growing human population, particularly in developing nations. This has triggered high-quality plant phenotyping re- search to help with the breeding of high yieldi... Read More about Towards infield, live plant phenotyping using a reduced-parameter CNN.

RootNav 2.0: Deep learning for automatic navigation of complex plant root architectures (2019)
Journal Article
Yasrab, R., Atkinson, J. A., Wells, D. M., French, A. P., Pridmore, T. P., & Pound, M. P. (2019). RootNav 2.0: Deep learning for automatic navigation of complex plant root architectures. GigaScience, 8(11), https://doi.org/10.1093/gigascience/giz123

© The Author(s) 2019. Published by Oxford University Press. BACKGROUND: In recent years quantitative analysis of root growth has become increasingly important as a way to explore the influence of abiotic stress such as high temperature and drought on... Read More about RootNav 2.0: Deep learning for automatic navigation of complex plant root architectures.

Cellular patterning of Arabidopsis roots under low phosphate conditions (2018)
Journal Article
Janes, G., von Wangenheim, D., Cowling, S., Kerr, I. D., Band, L. R., French, A. P., & Bishopp, A. (2018). Cellular patterning of Arabidopsis roots under low phosphate conditions. Frontiers in Plant Science, 9, Article 735. https://doi.org/10.3389/fpls.2018.00735

Phosphorus is a crucial macronutrient for plants playing a critical role in many cellular signaling and energy cycling processes. In light of this, phosphorus acquisition efficiency is an important target trait for crop improvement, but it also provi... Read More about Cellular patterning of Arabidopsis roots under low phosphate conditions.

Deep learning for multi-task plant phenotyping (2017)
Conference Proceeding
Pound, M. P., Atkinson, J. A., Wells, D. M., Pridmore, T. P., & French, A. P. (2017). Deep learning for multi-task plant phenotyping. In Proceedings of the IEEE International Conference on Computer Vision (ICCV), 2017 (2055-2063). https://doi.org/10.1109/ICCVW.2017.241

Plant phenotyping has continued to pose a challenge to computer vision for many years. There is a particular demand to accurately quantify images of crops, and the natural variability and structure of these plants presents unique difficulties. Recent... Read More about Deep learning for multi-task plant phenotyping.

Hyperspectral image analysis techniques for the detection and classification of the early onset of plant disease and stress (2017)
Journal Article
Lowe, A., Harrison, N., & French, A. P. (2017). Hyperspectral image analysis techniques for the detection and classification of the early onset of plant disease and stress. Plant Methods, 13, Article 80. https://doi.org/10.1186/s13007-017-0233-z

This review explores how imaging techniques are being developed with a focus on deployment for crop monitoring methods. Imaging applications are discussed in relation to both field and glasshouse-based plants, and techniques are sectioned into ‘healt... Read More about Hyperspectral image analysis techniques for the detection and classification of the early onset of plant disease and stress.

AutoRoot: open-source software employing a novel image analysis approach to support fully-automated plant phenotyping (2017)
Journal Article
Pound, M. P., Fozard, S., Torres Torres, M., Forde, B. G., & French, A. P. (2017). AutoRoot: open-source software employing a novel image analysis approach to support fully-automated plant phenotyping. Plant Methods, 13(1), Article 12. https://doi.org/10.1186/s13007-017-0161-y

Background: Computer-based phenotyping of plants has risen in importance in recent years. Whilst much software has been written to aid phenotyping using image analysis, to date the vast majority has been only semi-automatic. However, such interaction... Read More about AutoRoot: open-source software employing a novel image analysis approach to support fully-automated plant phenotyping.

Approaches to three-dimensional reconstruction of plant shoot topology and geometry (2016)
Journal Article
Gibbs, J., Pound, M. P., French, A. P., Wells, D. M., Murchie, E. H., & Pridmore, T. P. (2016). Approaches to three-dimensional reconstruction of plant shoot topology and geometry. Functional Plant Biology, 44(1), 62-75. https://doi.org/10.1071/FP16167

There are currently 805 million people classified as chronically undernourished, and yet the World’s population is still increasing. At the same time, global warming is causing more frequent and severe flooding and drought, thus destroying crops and... Read More about Approaches to three-dimensional reconstruction of plant shoot topology and geometry.

Deep Machine Learning provides state-of-the-art performance in image-based plant phenotyping (2016)
Working Paper
Pound, M. P., Burgess, A. J., Wilson, M. H., Atkinson, J. A., Griffiths, M., Jackson, A. S., …French, A. P. Deep Machine Learning provides state-of-the-art performance in image-based plant phenotyping

Deep learning is an emerging field that promises unparalleled results on many data analysis problems. We show the success offered by such techniques when applied to the challenging problem of image-based plant phenotyping, and demonstrate state-of-th... Read More about Deep Machine Learning provides state-of-the-art performance in image-based plant phenotyping.

A patch-based approach to 3D plant shoot phenotyping (2016)
Journal Article
Pound, M. P., French, A. P., Fozard, J. A., Murchie, E. H., & Pridmore, T. P. (2016). A patch-based approach to 3D plant shoot phenotyping. Machine Vision and Applications, 27(5), 767-779. https://doi.org/10.1007/s00138-016-0756-8

The emerging discipline of plant phenomics aims to measure key plant characteristics, or traits, though as yet the set of plant traits that should be measured by automated systems is not well defined. Methods capable of recovering generic representat... Read More about A patch-based approach to 3D plant shoot phenotyping.

Fast global interactive volume segmentation with regional supervoxel descriptors (2016)
Journal Article
Luengo, I., Basham, M., & French, A. P. (2016). Fast global interactive volume segmentation with regional supervoxel descriptors. Proceedings of SPIE, 9784, Article 97842D. https://doi.org/10.1117/12.2216382

In this paper we propose a novel approach towards fast multi-class volume segmentation that exploits supervoxels in order to reduce complexity, time and memory requirements. Current methods for biomedical image segmentation typically require either c... Read More about Fast global interactive volume segmentation with regional supervoxel descriptors.

Three-dimensional reconstruction of plant shoots from multiple images using an active vision system (2015)
Journal Article
Gibbs, J., Pound, M. P., Wells, D. M., Murchie, E. H., French, A. P., & Pridmore, T. P. (2015). Three-dimensional reconstruction of plant shoots from multiple images using an active vision system

The reconstruction of 3D models of plant shoots is a challenging problem central to the emerging discipline of plant phenomics – the quantitative measurement of plant structure and function. Current approaches are, however, often limited by the use o... Read More about Three-dimensional reconstruction of plant shoots from multiple images using an active vision system.

Quantification of Fluorescent Reporters in Plant Cells (2014)
Book Chapter
Pound, M., French, A. P., & Wells, D. M. (2015). Quantification of Fluorescent Reporters in Plant Cells. In J. M. Estevez (Ed.), Plant Cell Expansion: Methods and Protocols (123-131). Springer. https://doi.org/10.1007/978-1-4939-1902-4_11

© Springer Science+Business Media New York 2015. Fluorescent reporters are powerful tools for plant research. Many studies require accurate determination of fluorescence intensity and localization. Here, we describe protocols for the quantification o... Read More about Quantification of Fluorescent Reporters in Plant Cells.

Systems Analysis of Auxin Transport in the Arabidopsis Root Apex (2014)
Journal Article
Band, L. R., Wells, D. M., Fozard, J. A., Ghetiu, T., French, A. P., Pound, M. P., …Bennett, M. J. (2014). Systems Analysis of Auxin Transport in the Arabidopsis Root Apex. Plant Cell, 26(3), 862-875. https://doi.org/10.1105/tpc.113.119495

Auxin is a key regulator of plant growth and development. Within the root tip, auxin distribution plays a crucial role specifying developmental zones and coordinating tropic responses. Determining how the organ-scale auxin pattern is regulated at the... Read More about Systems Analysis of Auxin Transport in the Arabidopsis Root Apex.

Mechanical modelling quantifies the functional importance of outer tissue layers during root elongation and bending (2014)
Journal Article
Dyson, R. J., Vizcay-Barrena, G., Band, L. R., Fernandes, A. N., French, A. P., Fozard, J. A., …Jensen, O. E. (2014). Mechanical modelling quantifies the functional importance of outer tissue layers during root elongation and bending. New Phytologist, 202(4), 1212-1222. https://doi.org/10.1111/nph.12764

Root elongation and bending require the coordinated expansion of multiple cells of different types. These processes are regulated by the action of hormones that can target distinct cell layers. We use a mathematical model to characterise the influenc... Read More about Mechanical modelling quantifies the functional importance of outer tissue layers during root elongation and bending.

Sequential induction of auxin efflux and influx carriers regulates lateral root emergence (2013)
Journal Article
Peret, B., Middleton, A. M., French, A. P., Larrieu, A., Bishopp, A., Njo, M., …Bennett, M. J. (2013). Sequential induction of auxin efflux and influx carriers regulates lateral root emergence. Molecular Systems Biology, 9(1), Article 699. https://doi.org/10.1038/msb.2013.43

In Arabidopsis, lateral roots originate from pericycle cells deep within the primary root. New lateral root primordia (LRP) have to emerge through several overlaying tissues. Here, we report that auxin produced in new LRP is transported towards the o... Read More about Sequential induction of auxin efflux and influx carriers regulates lateral root emergence.