All Outputs (33)

Spatially resolved molecular analysis of host response to medical device implantation using the 3D OrbiSIMS highlights a critical role for lipids (2024)
Journal Article
Suvannapruk, W., Fisher, L. E., Luckett, J. C., Edney, M. K., Kotowska, A. M., Kim, D., …Alexander, M. R. (in press). Spatially resolved molecular analysis of host response to medical device implantation using the 3D OrbiSIMS highlights a critical role for lipids. Advanced Science, https://doi.org/10.1101/2023.08.18.553860

A key goal for implanted medical devices is that they do not elicit a detrimental immune response. Macrophages play critical roles in modulation of the host immune response and are the major cells responsible for persistent inflammatory reactions to... Read More about Spatially resolved molecular analysis of host response to medical device implantation using the 3D OrbiSIMS highlights a critical role for lipids.

Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model (2023)
Journal Article
Dooley, M., Luckett, J., Alexander, M. R., Matousek, P., Dehghani, H., Ghaemmaghami, A. M., & Notingher, I. (2023). Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model. Biomedical Optics Express, 14(12), 6592-6606. https://doi.org/10.1364/boe.512118

Diffuse Raman spectroscopy (DRS) allows subsurface molecular analysis of optically turbid samples. Numerical modeling of light propagation was used as a method for improving the design of an DRS instrument to maximize the signal to noise ratio (SNR)... Read More about Optimization of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in a small animal model.

Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model (2023)
Journal Article
Dooley, M., Luckett, J., Alexander, M. R., Matousek, P., Dehghani, H., Ghaemmaghami, A. M., & Notingher, I. (2023). Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model. Biomedical Optics Express, 14(12), 6592-6606. https://doi.org/10.1364/BOE.512118

Diffuse Raman spectroscopy (DRS) allows subsurface molecular analysis of optically turbid samples. Numerical modelling of light propagation was used as a method for improving the design of an DRS instrument to maximize the signal to noise ratio (SNR)... Read More about Optimisation of diffuse Raman spectroscopy for in-vivo quantification of foreign body response in small animal model.

Sustained Release of Dexamethasone from 3D-Printed Scaffolds Modulates Macrophage Activation and Enhances Osteogenic Differentiation (2023)
Journal Article
Majrashi, M., Yang, J., Ghaemmaghami, A., Kotowska, A., Hicks, J. M., & Scurr, D. (2023). Sustained Release of Dexamethasone from 3D-Printed Scaffolds Modulates Macrophage Activation and Enhances Osteogenic Differentiation. ACS Applied Materials and Interfaces, 15(49), 56623–56638. https://doi.org/10.1021/acsami.3c09774

Enhancing osteogenesis via modulating immune cells is emerging as a new approach to address current challenges in repairing bone defects and fractures. However, much remains unknown about the crosstalk between immune cells and osteolineage cells duri... Read More about Sustained Release of Dexamethasone from 3D-Printed Scaffolds Modulates Macrophage Activation and Enhances Osteogenic Differentiation.

Co-assembling living material as an in vitro lung epithelial infection model (2023)
Journal Article
Wu, Y., Romero, M., Robertson, S. N., Fenn, S., Fisher, L., Willingham, I., …Mata, A. (2024). Co-assembling living material as an in vitro lung epithelial infection model. Matter, 7(1), 216-236. https://doi.org/10.1016/j.matt.2023.10.029

Biofilms are robust living 3D materials that play key roles in nature but also cause major problems, such as tolerance to antibiotic treatment. Recreation of these living structures in vitro is critical to understand their biology and develop solutio... Read More about Co-assembling living material as an in vitro lung epithelial infection model.

Modelled-Microgravity Reduces Virulence Factor Production in Staphylococcus aureus through Downregulation of agr-Dependent Quorum Sensing (2023)
Journal Article
Green, M. J., Murray, E. J., Williams, P., Ghaemmaghami, A. M., Aylott, J. W., & Williams, P. M. (2023). Modelled-Microgravity Reduces Virulence Factor Production in Staphylococcus aureus through Downregulation of agr-Dependent Quorum Sensing. International Journal of Molecular Sciences, 24(21), Article 15997. https://doi.org/10.3390/ijms242115997

Bacterial contamination during space missions is problematic for human health and damages filters and other vital support systems. Staphylococcus aureus is both a human commensal and an opportunistic pathogen that colonizes human tissues and causes a... Read More about Modelled-Microgravity Reduces Virulence Factor Production in Staphylococcus aureus through Downregulation of agr-Dependent Quorum Sensing.

Innate immune cell instruction using micron-scale 3D objects of varied architecture and polymer chemistry: The ChemoArchiChip (2023)
Journal Article
Vassey, M., Ma, L., Kämmerling, L., Mbadugha, C., Trindade, G. F., Figueredo, G. P., …Alexander, M. R. (2023). Innate immune cell instruction using micron-scale 3D objects of varied architecture and polymer chemistry: The ChemoArchiChip. Matter, 6(3), 887-906. https://doi.org/10.1016/j.matt.2023.01.002

To design effective immunomodulatory implants, innate immune cell interactions at the surface of biomaterials need to be controlled and understood. The architectural design freedom of two-photon polymerization is used to produce arrays of surface-mou... Read More about Innate immune cell instruction using micron-scale 3D objects of varied architecture and polymer chemistry: The ChemoArchiChip.

Microparticles Decorated with Cell‐Instructive Surface Chemistries Actively Promote Wound Healing (2022)
Journal Article
Latif, A., Fisher, L. E., Dundas, A. A., Crucitti, V. C., Imir, Z., Lawler, K., …Ghaemmaghami, A. M. (2022). Microparticles Decorated with Cell‐Instructive Surface Chemistries Actively Promote Wound Healing. Advanced Materials, Article 2208364. https://doi.org/10.1002/adma.202208364

Wound healing is a complex biological process involving close crosstalk between various cell types. Dysregulation in any of these processes, such as in diabetic wounds, results in chronic nonhealing wounds. Fibroblasts are a critical cell type involv... Read More about Microparticles Decorated with Cell‐Instructive Surface Chemistries Actively Promote Wound Healing.

Metabolic Signatures of Surface-Modified Poly(lactic- co-glycolic acid) Nanoparticles in Differentiated THP-1 Cells Derived with Liquid Chromatography-Mass Spectrometry-based Metabolomics (2022)
Journal Article
Al-Natour, M. A., Abdelrazig, S., Ghaemmaghami, A. M., Alexander, C., & Kim, D. H. (2022). Metabolic Signatures of Surface-Modified Poly(lactic- co-glycolic acid) Nanoparticles in Differentiated THP-1 Cells Derived with Liquid Chromatography-Mass Spectrometry-based Metabolomics. ACS Omega, https://doi.org/10.1021/acsomega.2c01660

Polymeric nanoparticles (NPs) are widely used in preclinical drug delivery investigations, and some formulations are now in the clinic. However, the detailed effects of many NPs at the subcellular level have not been fully investigated. In this study... Read More about Metabolic Signatures of Surface-Modified Poly(lactic- co-glycolic acid) Nanoparticles in Differentiated THP-1 Cells Derived with Liquid Chromatography-Mass Spectrometry-based Metabolomics.

Mineralizing Coating on 3D Printed Scaffolds for the Promotion of Osseointegration (2022)
Journal Article
Hasan, A., Bagnol, R., Owen, R., Latif, A., Rostam, H. M., Elsharkawy, S., …Mata, A. (2022). Mineralizing Coating on 3D Printed Scaffolds for the Promotion of Osseointegration. Frontiers in Bioengineering and Biotechnology, 10, Article 836386. https://doi.org/10.3389/fbioe.2022.836386

Design and fabrication of implants that can perform better than autologous bone grafts remain an unmet challenge for the hard tissue regeneration in craniomaxillofacial applications. Here, we report an integrated approach combining additive manufactu... Read More about Mineralizing Coating on 3D Printed Scaffolds for the Promotion of Osseointegration.

Single-Cell Metabolic Profiling of Macrophages Using 3D OrbiSIMS: Correlations with Phenotype (2022)
Journal Article
Suvannapruk, W., Edney, M. K., Kim, D., Scurr, D. J., Ghaemmaghami, A. M., & Alexander, M. R. (2022). Single-Cell Metabolic Profiling of Macrophages Using 3D OrbiSIMS: Correlations with Phenotype. Analytical Chemistry, 94(26), 9389–9398. https://doi.org/10.1021/acs.analchem.2c01375

Macrophages are important immune cells that respond to environmental cues acquiring a range of activation statuses represented by pro-inflammatory (M1) and anti-inflammatory (M2) phenotypes at each end of their spectrum. Characterizing the metabolic... Read More about Single-Cell Metabolic Profiling of Macrophages Using 3D OrbiSIMS: Correlations with Phenotype.

Immune-instructive materials as new tools for immunotherapy (2021)
Journal Article
Fisher, L. E., Kämmerling, L., Alexander, M. R., & Ghaemmaghami, A. M. (2022). Immune-instructive materials as new tools for immunotherapy. Current Opinion in Biotechnology, 74, 194-203. https://doi.org/10.1016/j.copbio.2021.11.005

Immune instructive materials, are materials with the ability to modulate or mimic the function of immune cells, provide exciting opportunities for developing new therapies in many areas including medical devices, chronic inflammation, cancer, and aut... Read More about Immune-instructive materials as new tools for immunotherapy.

Discovery of synergistic material-topography combinations to achieve immunomodulatory osteoinductive biomaterials using a novel in vitro screening method: The ChemoTopoChip (2021)
Journal Article
Burroughs, L., Amer, M., Vassey, M., Koch, B., Figueredo, G., Mukonoweshuro, B., …Alexander, M. R. (2021). Discovery of synergistic material-topography combinations to achieve immunomodulatory osteoinductive biomaterials using a novel in vitro screening method: The ChemoTopoChip. Biomaterials, 271, Article 120740. https://doi.org/10.1016/j.biomaterials.2021.120740

© 2021 The Authors Human mesenchymal stem cells (hMSCs) are widely represented in regenerative medicine clinical strategies due to their compatibility with autologous implantation. Effective bone regeneration involves crosstalk between macrophages an... Read More about Discovery of synergistic material-topography combinations to achieve immunomodulatory osteoinductive biomaterials using a novel in vitro screening method: The ChemoTopoChip.

Immunity in space: prokaryote adaptations and immune response in microgravity (2021)
Journal Article
Green, M. J., Aylott, J. W., Williams, P., Ghaemmaghami, A. M., & Williams, P. M. (2021). Immunity in space: prokaryote adaptations and immune response in microgravity. Life, 11(2), Article 112. https://doi.org/10.3390/life11020112

Immune dysfunction has long been reported by medical professionals regarding astronauts suffering from opportunistic infections both during their time in space and a short period afterwards once back on Earth. Various species of prokaryotes onboard t... Read More about Immunity in space: prokaryote adaptations and immune response in microgravity.

Corrigendum to ‘LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate’ [International Journal of Pharmaceutics: X Volume 1 (2019) 100036] (2020)
Journal Article
Al-Natour, M. A., Alazzo, A., Ghaemmaghami, A. M., Kim, D. H., & Alexander, C. (2020). Corrigendum to ‘LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate’ [International Journal of Pharmaceutics: X Volume 1 (2019) 100036]. International Journal of Pharmaceutics: X, 2, Article 100044. https://doi.org/10.1016/j.ijpx.2020.100044

© 2020 The Author(s) The authors regret that the original version of Fig. 3 incorrect. The updated figure is below for reference. [Figure presented] Fig. 3 Super-resolution microscopy image of methotrexate loaded PLGA nanoparticles (green) (92 μg/mL,... Read More about Corrigendum to ‘LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate’ [International Journal of Pharmaceutics: X Volume 1 (2019) 100036].

Developing immune-regulatory materials using immobilized monosaccharides with immune-instructive properties (2020)
Journal Article
Alobaid, M. A., Richards, S., Alexander, M. R., Gibson, M. I., & Ghaemmaghami, A. M. (2020). Developing immune-regulatory materials using immobilized monosaccharides with immune-instructive properties. Materials Today Bio, 8, https://doi.org/10.1016/j.mtbio.2020.100080

© 2020 The Authors New strategies for immune modulation have shown real promise in regenerative medicine as well as the fight against autoimmune diseases, allergies, and cancer. Dendritic cells (DCs) are gatekeepers of the immune system and their abi... Read More about Developing immune-regulatory materials using immobilized monosaccharides with immune-instructive properties.

Achieving Microparticles with Cell-Instructive Surface Chemistry by Using Tunable Co-Polymer Surfactants (2020)
Journal Article
Dundas, A. A., Cuzzucoli Crucitti, V., Haas, S., Dubern, J., Latif, A., Romero, M., …Irvine, D. J. (2020). Achieving Microparticles with Cell-Instructive Surface Chemistry by Using Tunable Co-Polymer Surfactants. Advanced Functional Materials, 30(36), https://doi.org/10.1002/adfm.202001821

© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim A flow-focusing microfluidic device is used to produce functionalized monodisperse polymer particles with surface chemistries designed to control bacterial biofilm formatio... Read More about Achieving Microparticles with Cell-Instructive Surface Chemistry by Using Tunable Co-Polymer Surfactants.

Facile Dye-Initiated Polymerization of Lactide–Glycolide Generates Highly Fluorescent Poly(lactic-co-glycolic Acid) for Enhanced Characterization of Cellular Delivery (2020)
Journal Article
Al-Natour, M. A., Yousif, M. D., Cavanagh, R., Abouselo, A., Apebende, E. A., Ghaemmaghami, A., …Alexander, C. (2020). Facile Dye-Initiated Polymerization of Lactide–Glycolide Generates Highly Fluorescent Poly(lactic-co-glycolic Acid) for Enhanced Characterization of Cellular Delivery. ACS Macro Letters, 9(3), 431-437. https://doi.org/10.1021/acsmacrolett.9b01014

Copyright © 2020 American Chemical Society. Poly(lactic-co-glycolic acid) (PLGA) is a versatile synthetic copolymer that is widely used in pharmaceutical applications. This is because it is well-tolerated in the body, and copolymers of varying physic... Read More about Facile Dye-Initiated Polymerization of Lactide–Glycolide Generates Highly Fluorescent Poly(lactic-co-glycolic Acid) for Enhanced Characterization of Cellular Delivery.

LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate (2019)
Journal Article
Al-Natour, M. A., Alazzo, A., Ghaemmaghami, A. M., Kim, D., & Alexander, C. (2019). LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate. International Journal of Pharmaceutics: X, 1, Article 100036. https://doi.org/10.1016/j.ijpx.2019.100036

Methotrexate (MTX) is a folate analogue antimetabolite widely used for the treatment of rheumatoid arthritis and cancer. A number of studies have shown that MTX delivered via nanoparticle carriers is more potent against cancer cells than free MTX, a... Read More about LC-MS metabolomics comparisons of cancer cell and macrophage responses to methotrexate and polymer-encapsulated methotrexate.

Nanofibrous scaffolds support a 3D in vitro permeability model of the human intestinal epitheleum (2019)
Journal Article
Patient, J. D., Hajiali, H., Harris, K., Abrahamsson, B., Tannergreen, C., White, L. J., …Rose, F. R. (2019). Nanofibrous scaffolds support a 3D in vitro permeability model of the human intestinal epitheleum. Frontiers in Pharmacology, 10, Article 456. https://doi.org/10.3389/fphar.2019.00456

Advances in drug research not only depend on high throughput screening to evaluate large numbers of lead compounds but also on the development of in vitro models which can simulate human tissues in terms of drug permeability and functions. Potential... Read More about Nanofibrous scaffolds support a 3D in vitro permeability model of the human intestinal epitheleum.