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Nucleotide exchange factor Rab3GEP requires DENN and non-DENN elements for activation and targeting of Rab27a (2019)
Journal Article
Sanzà, P., Evans, R. D., Briggs, D. A., Cantero, M., Montoliu, L., Patel, S., …Hume, A. N. (2019). Nucleotide exchange factor Rab3GEP requires DENN and non-DENN elements for activation and targeting of Rab27a. Journal of Cell Science, 132(9), 1-8. doi:10.1242/jcs.212035

Rab GTPases are compartment-specific molecular switches that regulate intracellular vesicular transport in eukaryotes. GDP/GTP exchange factors (GEFs) control Rab activation, and current models propose that localised and regulated GEF activity is imp... Read More about Nucleotide exchange factor Rab3GEP requires DENN and non-DENN elements for activation and targeting of Rab27a.

The adaptor protein melanophilin regulates dynamic myosin- Va: cargo interaction and dendrite development in melanocytes (2019)
Journal Article
Robinson, C. L., Evans, R. D., Sivarasa, K., Ramalho, J. S., Briggs, D. A., & Hume, A. N. (2019). The adaptor protein melanophilin regulates dynamic myosin- Va: cargo interaction and dendrite development in melanocytes. Molecular Biology of the Cell, 30(6), 731-809. doi:10.1091/mbc.E18-04-0237

Regulation of organelle transport by the cytoskeleton is fundamental for eukaryotic survival. Cytoskeleton motors are typically modular proteins with conserved motor and diverse cargo binding domains. Motor:cargo interactions are often indirect and m... Read More about The adaptor protein melanophilin regulates dynamic myosin- Va: cargo interaction and dendrite development in melanocytes.

Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport (2017)
Journal Article
Robinson, C. L., Evans, R. D., Briggs, D. A., Ramalho, J. S., & Hume, A. N. (in press). Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport. Journal of Cell Science, 130(12), https://doi.org/10.1242/jcs.186064

Microtubules and F-actin, and associated motor proteins, are considered to play complementary roles in long- and short-range organelle transport. However, there is growing appreciation that myosin/F-actin networks can drive long-range transport. In m... Read More about Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport.

Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes (2016)
Journal Article
Pylypenko, O., Welz, T., Tittel, J., Kollmar, M., Chardon, F., Malherbe, G., …Kerkhoff, E. (in press). Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes. eLife, 5, https://doi.org/10.7554/eLife.17523

There is growing evidence for a coupling of actin assembly and myosin motor activity in cells. However, mechanisms for recruitment of actin nucleators and motors on specific membrane compartments remain unclear. Here we report how Spir actin nucleato... Read More about Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes.

A role for Rab27 in neutrophil chemotaxis and lung recruitment (2014)
Journal Article
Seabra, M. C., Rankin, S. M., Birrell, M. A., Furze, R. C., Singh, R. K., & Hume, A. N. (2014). A role for Rab27 in neutrophil chemotaxis and lung recruitment. BMC Cell Biology, 15(1), https://doi.org/10.1186/s12860-014-0039-z

© 2014 Singh et al. Background: Neutrophils are a critical part of the innate immune system. Their ability to migrate into infected or injured tissues precedes their role in microbial killing and clearance. We have previously shown that Rab27a can pr... Read More about A role for Rab27 in neutrophil chemotaxis and lung recruitment.

Myosin-Va and dynamic actin oppose microtubules to drive long-range organelle transport (2014)
Journal Article
Evans, R. D., Robinson, C., Briggs, D. A., Tooth, D. J., Ramalho, J. S., Cantero, M., …Hume, A. N. (2014). Myosin-Va and dynamic actin oppose microtubules to drive long-range organelle transport. Current Biology, 24(15), 1743-1750. https://doi.org/10.1016/j.cub.2014.06.019

In animal cells, microtubule and actin tracks and their associated motors (dynein, kinesin, and myosin) are thought to regulate long- and short-range transport, respectively [1-8]. Consistent with this, microtubules extend from the perinuclear centro... Read More about Myosin-Va and dynamic actin oppose microtubules to drive long-range organelle transport.

A role for Na+,K+-ATPase α1 in regulating Rab27a localisation on melanosomes (2014)
Journal Article
Seabra, M. C., Tarafder, A. K., Booth, A. E., Hume, A. N., & Recchi, C. (2014). A role for Na+,K+-ATPase α1 in regulating Rab27a localisation on melanosomes. PLoS ONE, 9(7), https://doi.org/10.1371/journal.pone.0102851

The mechanism(s) by which Rab GTPases are specifically recruited to distinct intracellular membranes remains elusive. Here we used Rab27a localisation onto melanosomes as a model to investigate Rab targeting. We identified the α1 subunit of Na+,K+-AT... Read More about A role for Na+,K+-ATPase α1 in regulating Rab27a localisation on melanosomes.

Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosis (2013)
Journal Article
Hume, A. N., Tarafder, A. K., Bolasco, G., Correia, M. S., Pereira, F. J., Iannone, L., …Seabra, M. C. (2014). Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosis. Journal of Investigative Dermatology, 134(4), 1056-1066. https://doi.org/10.1038/jid.2013.432

The transfer of melanin from melanocytes to keratinocytes is a crucial process underlying maintenance of skin pigmentation and photoprotection against UV damage. Here, we present evidence supporting coupled exocytosis of the melanin core, or melanoco... Read More about Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosis.