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Adipogenic differentiation of muscle derived cells is repressed by inhibition of GSK-3 activity

Redshaw, Zoe; Loughna, Paul Thomas


Zoe Redshaw

Paul Thomas Loughna


Background: Intramuscular fat is important in large animal livestock species in regard to meat quality and in humans is of clinical significance in particular in relation to insulin resistance. The canonical Wnt signalling pathway has been implicated at a whole body level in regulating relative levels of adiposity versus lean body mass. Previously we have shown that pig muscle cells can undergo adipogenic differentiation to a degree that is dependent upon the specific muscle source. In this work we examine the role of the canonical Wnt pathway which acts through inactivation of glycogen synthase kinase-3 (GSK-3) in the regulation of adipogenic differentiation in muscle cells derived from the pig semimembranosus muscle.
Results: The application of lithium chloride to muscle derived cells significantly increased the phosphorylation of GSK-3??and thus inhibited its activity thus mimicking Wnt signaling. This was associated with a significant decrease in the expression of the adipogenic transcription factor PPAR??and an almost complete inhibition of adipogenesis in the cells. The data also suggest that GSK-3??plays, at most, a small role in this process.
Conclusions: Studies in vivo have suggested that the Wnt pathway is a major regulator of whole body adiposity. In this study we have shown that the ability of cells derived from porcine skeletal muscle to differentiate along an adipogenic lineage, in vitro, is severely impaired by mimicking the action of this pathway. This was done by inactivation of GSK-3??by the use of Lithium Chloride.


Redshaw, Z., & Loughna, P. T. (2018). Adipogenic differentiation of muscle derived cells is repressed by inhibition of GSK-3 activity. Frontiers in Veterinary Science, 5, doi:10.3389/fvets.2018.00110. ISSN 2297-1769

Journal Article Type Article
Acceptance Date May 4, 2018
Online Publication Date Jun 12, 2018
Publication Date Jun 12, 2018
Deposit Date Aug 10, 2018
Publicly Available Date Aug 10, 2018
Journal Frontiers in Veterinary Science
Electronic ISSN 2297-1769
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 5
Article Number 110
Publisher URL


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