Application of 'smart' amine donors for rapid screening and scale-up of transaminase-mediated biotransformations

Gomm, Andrew; Grigoriou, Stylianos; Peel, Christopher; Ryan, James; Mujtaba, Nafees; Clarke, Thomas; Kulcinskaja, Evelina; O'Reilly, Elaine

Abstract

The 'smart' amine donors o‐xylylenediamine and cadaverine were employed for the rapid screening of a large ketone library and subsequent preparative‐scale synthesis of selected compounds using the commercially available amine transaminase, ATA256. The methodology enables both screening and preparative‐scale biotransformations to be performed with a single enzyme and simplifies the generation of sp3‐rich small molecule libraries.

Citation

Gomm, A., Grigoriou, S., Peel, C., Ryan, J., Mujtaba, N., Clarke, T., …O'Reilly, E. (2018). Application of 'smart' amine donors for rapid screening and scale-up of transaminase-mediated biotransformations. European Journal of Organic Chemistry, 2018(38), 5282-5284. https://doi.org/10.1002/ejoc.201800799

• Journal Article Type: Article
• Acceptance Date: Jul 6, 2018
• Online Publication Date: Jul 6, 2018
• Publication Date: Oct 17, 2018
• Deposit Date: Aug 10, 2018
• Publicly Available Date: Jul 7, 2019
• Journal: European Journal of Organic Chemistry
• Print ISSN: 1434-193X
• Electronic ISSN: 1099-0690
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 2018
• Issue: 38
• Pages: 5282-5284
• DOI: https://doi.org/10.1002/ejoc.201800799
• Keywords: Physical and theoretical chemistry; Organic chemistry
• Public URL: https://nottingham-repository.worktribe.com/output/989077
• Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/ejoc.201800799
• Additional Information: This is the pre-peer reviewed version of the following article: Gomm, A. , Grigoriou, S. , Peel, C. , Ryan, J. , Mujtaba, N. , Clarke, T. , Evelina Kulcinskaja, E. and O'Reilly, E. (2018), Application of 'smart' amine donors for rapid screening and scale‐up of transaminase‐mediated biotransformations. Eur. J. Org. Chem, which has been published in final form at https://onlinelibrary.wiley.com/doi/abs/10.1002/ejoc.201800799. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.