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A new HPLC method for simultaneously measuring chloride, sugars, organic acids and alcohols in food samples

Zaky, Abdelrahman Saleh; Pensupa, Nattha; Andrade-Eiroa, �urea; Tucker, Gregory A.; Du, Chenyu

A new HPLC method for simultaneously measuring chloride, sugars, organic acids and alcohols in food samples Thumbnail


Authors

Abdelrahman Saleh Zaky

Nattha Pensupa

�urea Andrade-Eiroa

Gregory A. Tucker

Chenyu Du



Abstract

This paper introduces an original, rapid, efficient and reliable HPLC method for the accurate and simultaneous quantification (g/L) of chloride in samples containing sugars, organic acids and alcohols. Separation was achieved using a HI-Plex H column at 35oC, with H2SO4 (0.005 N) as the mobile phase at a flow rate of 0.4 mL/min. The column effluent was monitored by a Refractive Index (RI) detector. A linear response was achieved over NaCl concentrations of 0.25 – 2.5 g/L and 5 – 40 g/L. The analytical method inter- and intra-run accuracy and precision were better than ±10.0%. Investigating the mechanism of detection using different chloride and sodium s reviled that this method can be used for determining the total concentration of chloride salts when in suspension. This method was successfully applied to 15 samples of commercial food products and the salt content obtained from this method was compared with 3 other methods for salt determination. The (HI-Plex H) column was designed for determining the concentrations of sugars, organic acids and alcohols when in solution. Hence, application of our new methodology would allow the determination of sugars, alcohols and organic acids in samples derived from seawater-based fermentation media as well as samples from salty food and dairy products.

Citation

Zaky, A. S., Pensupa, N., Andrade-Eiroa, Á., Tucker, G. A., & Du, C. (2017). A new HPLC method for simultaneously measuring chloride, sugars, organic acids and alcohols in food samples. Journal of Food Composition and Analysis, 56, https://doi.org/10.1016/j.jfca.2016.12.010

Journal Article Type Article
Acceptance Date Dec 12, 2016
Online Publication Date Dec 12, 2016
Publication Date Mar 1, 2017
Deposit Date Dec 19, 2016
Publicly Available Date Mar 28, 2024
Journal Journal of Food Composition and Analysis
Print ISSN 0889-1575
Electronic ISSN 0889-1575
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 56
DOI https://doi.org/10.1016/j.jfca.2016.12.010
Keywords Food analysis; Food composition; High Performance Liquid Chromatography (HPLC); Cl-Determination; Sugar analysis, Hi-Plex H column; Seawater; Salts meter; Ion chromatography (IC); Flame photometer (FP)
Public URL https://nottingham-repository.worktribe.com/output/970481
Publisher URL http://www.sciencedirect.com/science/article/pii/S0889157516302265

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