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Community recommendations on terminology and procedures used in flooding and low oxygen stress research

Sasidharan, Rashmi; Bailey-Serres, Julia; Ashikari, Motoyuki; Atwell, Brian J.; Colmer, Timothy D.; Fagerstedt, Kurt; Fukao, Takeshi; Geigenberger, Peter; Hebelstrup, Kim H.; Hill, Robert D.; Holdsworth, Michael J.; Ismail, Abdelbagi M.; Licausi, Francesco; Mustroph, Angelika; Nakazono, Mikio; Pedersen, Ole; Perata, Pierdomenico; Sauter, Margret; Shih, Ming-Che; Sorrell, Brian K.; Striker, Gustavo G.; van Dongen, Joost T.; Whelan, James; Xiao, Shi; Visser, Eric J. W.; Voesenek, Laurentius A. C. J.

Authors

Rashmi Sasidharan

Julia Bailey-Serres

Motoyuki Ashikari

Brian J. Atwell

Timothy D. Colmer

Kurt Fagerstedt

Takeshi Fukao

Peter Geigenberger

Kim H. Hebelstrup

Robert D. Hill

Abdelbagi M. Ismail

Francesco Licausi

Angelika Mustroph

Mikio Nakazono

Ole Pedersen

Pierdomenico Perata

Margret Sauter

Ming-Che Shih

Brian K. Sorrell

Gustavo G. Striker

Joost T. van Dongen

James Whelan

Shi Xiao

Eric J. W. Visser

Laurentius A. C. J. Voesenek



Abstract

Apart from playing a key role in important biochemical reactions, molecular oxygen (O2) and its by-products also have crucial signaling roles in shaping plant developmental programs and environmental responses. Even under normal conditions, sharp O2 gradients can occur within the plant when cellular O2 demand exceeds supply, especially in dense organs such as tubers, seeds and fruits. Spatial and temporal variations in O2 concentrations are important cues for plants to modulate development (van Dongen & Licausi, 2015; Considine et al., 2016). Environmental conditions can also expand the low O2 regions within the plant. For example, excessive rainfall can lead to partial or complete plant submergence resulting in O2 deficiency in the root or the entire plant (Voesenek & Bailey-Serres, 2015). Climate change-associated increases in precipitation events have made flooding a major abiotic stress threatening crop production and food sustainability. This increased flooding and associated crop losses highlight the urgency of understanding plant flooding responses and tolerance mechanisms.
Timely manifestation of physiological and morphological changes triggering developmental adjustments or flooding survival strategies requires accurate sensing of O2 levels. Despite progress in understanding how plants sense and respond to changes in intracellular O2 concentrations (van Dongen & Licausi, 2015), several questions remain unanswered due to a lack of high resolution tools to accurately and noninvasively monitor (sub)cellular O2 concentrations. In the absence of such tools, it is therefore critical for researchers in the field to be aware of how experimental conditions can influence plant O2 levels, and thus on the importance of accurately reporting specific experimental details. This also requires a consensus on the definition of frequently used terms.
At the 15th New Phytologist Workshop on Flooding stress (Voesenek et al., 2016), community members discussed and agreed on unified nomenclature and standard norms for low O2 and flooding stress research. This consensus on terminology and experimental guidelines is presented here. We expect that these norms will facilitate more effective interpretation, comparison and reproducibility of research in this field. We also highlight the current challenges in noninvasively monitoring and measuring O2 concentrations in plant cells, outlining the technologies currently available, their strengths and drawbacks, and their suitability for use in flooding and low O2 research.

Citation

Sasidharan, R., Bailey-Serres, J., Ashikari, M., Atwell, B. J., Colmer, T. D., Fagerstedt, K., …Voesenek, L. A. C. J. (2017). Community recommendations on terminology and procedures used in flooding and low oxygen stress research. New Phytologist, 214(4), 1403-1407. https://doi.org/10.1111/nph.14519

Journal Article Type Article
Acceptance Date Mar 9, 2017
Online Publication Date Mar 9, 2017
Publication Date 2017-06
Deposit Date Mar 15, 2017
Publicly Available Date Mar 15, 2017
Journal New Phytologist
Print ISSN 0028-646X
Electronic ISSN 1469-8137
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 214
Issue 4
Pages 1403-1407
DOI https://doi.org/10.1111/nph.14519
Keywords anoxia; flooding; hypoxia; low oxygen; plant anaerobiosis; reoxygenation; submergence; waterlogging
Public URL http://eprints.nottingham.ac.uk/id/eprint/41320
Publisher URL http://onlinelibrary.wiley.com/doi/10.1111/nph.14519/full
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf
Additional Information This is the peer reviewed version of the following article: Sasidharan, R., Bailey-Serres, J., Ashikari, M., Atwell, B. J., Colmer, T. D., Fagerstedt, K., Fukao, T., Geigenberger, P., Hebelstrup, K. H., Hill, R. D., Holdsworth, M. J., Ismail, A. M., Licausi, F., Mustroph, A., Nakazono, M., Pedersen, O., Perata, P., Sauter, M., Shih, M.-C., Sorrell, B. K., Striker, G. G., van Dongen, J. T., Whelan, J., Xiao, S., Visser, E. J. W. and Voesenek, L. A. C. J. (2017), Community recommendations on terminology and procedures used in flooding and low oxygen stress research. New Phytol., which has been published in final form at http://dx.doi.org/10.1111/nph.14519. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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