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Development of a SERS strategy to overcome the nanoparticle stabilisation effect in serum-containing samples: Application to the quantification of dopamine in the culture medium of PC-12 cells

Dumont, E.; De Bleye, C.; Cailletaud, J.; Sacre, P.-Y.; Van Lerberghe, P.-B.; Rogister, B.; Rance, G.A.; Aylott, J.W.; Hubert, Ph.; Ziemons, E.

Authors

E. Dumont

C. De Bleye

J. Cailletaud

P.-Y. Sacre

P.-B. Van Lerberghe

B. Rogister

Ph. Hubert

E. Ziemons



Abstract

The analysis of serum samples by surface-enhanced Raman spectroscopy (SERS) has gained ground over the last years. However, the stabilisation of colloids by the proteins contained in these samples has restricted their use in common practice, unless antibodies or aptamers are used. Therefore, this work was dedicated to the development of a SERS methodology allowing the analysis of serum samples in a simple and easy-to-implement way. This approach was based on the pre-aggregation of the colloid with a salt solution. Gold nanoparticles (AuNPs) were used as the SERS substrate and, owing to its physiopathological importance, dopamine was chosen as a model to implement the SERS approach. The presence of this neurotransmitter could be determined in the concentration range 0.5 to 50 ppm (2.64 – 264 μM) in the culture medium of PC-12 cells, with a R2 of 0.9874, and even at lower concentrations (0.25 ppm, 1.32 μM) in another matrix containing fewer proteins. Moreover, the effect of calcium and potassium on the dopamine exocytosis from PC-12 cells was studied. Calcium was shown to have a predominant and dose-dependent effect. Finally, PC-12 cells were exposed to dexamethasone in order to increase their biosynthesis and release of dopamine. This increase was monitored with the developed SERS approach.

Citation

Dumont, E., De Bleye, C., Cailletaud, J., Sacre, P., Van Lerberghe, P., Rogister, B., …Ziemons, E. (2018). Development of a SERS strategy to overcome the nanoparticle stabilisation effect in serum-containing samples: Application to the quantification of dopamine in the culture medium of PC-12 cells. Talanta, 186, https://doi.org/10.1016/j.talanta.2018.04.038

Journal Article Type Article
Acceptance Date Apr 8, 2018
Online Publication Date Apr 10, 2018
Publication Date Aug 15, 2018
Deposit Date Jul 3, 2018
Publicly Available Date Apr 11, 2019
Journal Talanta
Print ISSN 0039-9140
Electronic ISSN 0039-9140
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 186
DOI https://doi.org/10.1016/j.talanta.2018.04.038
Keywords Surface-Enhanced Raman Scattering (SERS); Gold nanoparticles; Dopamine; Quantitative methods; Cellular analysis; PC-12 cells
Public URL https://nottingham-repository.worktribe.com/output/949018
Publisher URL https://www.sciencedirect.com/science/article/pii/S0039914018303795

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





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