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Optical sectioning in multi-foci Raman hyperspectral imaging

Liao, Zhiyu; Sinjab, Faris; Elsheikha, Hany M.; Notingher, Ioan


Zhiyu Liao

Faris Sinjab


In this study, we compared the depth-discrimination and speed performance of multi-foci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator (LC-SLM) was employed for the generation of multi-foci laser beams, and a digital micromirror device (DMD) was used as a software-configurable reflective pinhole array. The patterns of the laser-foci and pinhole array can be rapidly changed without requiring any hardware alterations. Confocal patterns with different distance-to-size ratios were tested and compared. After optimisation of the laser foci pattern, we demonstrated the feasibility of multi-foci Raman hyperspectral microscopy for recording depth-resolved molecular maps of biological cells (Acanthamoeba castellanii trophozoites). Micrometric depth-discrimination and short acquisition times (20 minutes for single plane confocal image) was achieved.


Liao, Z., Sinjab, F., Elsheikha, H. M., & Notingher, I. (2018). Optical sectioning in multi-foci Raman hyperspectral imaging. Journal of Raman Spectroscopy, 49(10), 1660-1667.

Journal Article Type Article
Acceptance Date Jun 20, 2018
Online Publication Date Jul 18, 2018
Publication Date 2018-10
Deposit Date Jul 5, 2018
Publicly Available Date Jul 18, 2018
Journal Journal of Raman Spectroscopy
Print ISSN 0377-0486
Electronic ISSN 1097-4555
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 49
Issue 10
Pages 1660-1667
Keywords Confocal Raman imaging; optical sectioning; multi-beam; cross-talk; single cell imaging
Public URL
Publisher URL


Liao_et_al-2018-Journal_of_Raman_Spectroscopy.pdf (952 Kb)

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