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Optical sectioning in multi-foci Raman hyperspectral imaging

Liao, Zhiyu; Sinjab, Faris; Elsheikha, Hany M.; Notingher, Ioan

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Zhiyu Liao

Faris Sinjab


In this study, we compared the depth-discrimination and speed performance of multi-foci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator (LC-SLM) was employed for the generation of multi-foci laser beams, and a digital micromirror device (DMD) was used as a software-configurable reflective pinhole array. The patterns of the laser-foci and pinhole array can be rapidly changed without requiring any hardware alterations. Confocal patterns with different distance-to-size ratios were tested and compared. After optimisation of the laser foci pattern, we demonstrated the feasibility of multi-foci Raman hyperspectral microscopy for recording depth-resolved molecular maps of biological cells (Acanthamoeba castellanii trophozoites). Micrometric depth-discrimination and short acquisition times (20 minutes for single plane confocal image) was achieved.

Journal Article Type Article
Acceptance Date Jun 20, 2018
Online Publication Date Jul 18, 2018
Publication Date 2018-10
Deposit Date Jul 5, 2018
Publicly Available Date Jul 18, 2018
Journal Journal of Raman Spectroscopy
Print ISSN 0377-0486
Electronic ISSN 1097-4555
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 49
Issue 10
Pages 1660-1667
Keywords Confocal Raman imaging; optical sectioning; multi-beam; cross-talk; single cell imaging
Public URL
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