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Optical sectioning in multi-foci Raman hyperspectral imaging

Liao, Zhiyu; Sinjab, Faris; Elsheikha, Hany M.; Notingher, Ioan

Authors

Zhiyu Liao zhiyu.liao@nottingham.ac.uk

Faris Sinjab faris.sinjab@nottingham.ac.uk



Abstract

In this study, we compared the depth-discrimination and speed performance of multi-foci Raman hyperspectral imaging with the reference standard of a single laser point confocal Raman mapping. A liquid crystal spatial light modulator (LC-SLM) was employed for the generation of multi-foci laser beams, and a digital micromirror device (DMD) was used as a software-configurable reflective pinhole array. The patterns of the laser-foci and pinhole array can be rapidly changed without requiring any hardware alterations. Confocal patterns with different distance-to-size ratios were tested and compared. After optimisation of the laser foci pattern, we demonstrated the feasibility of multi-foci Raman hyperspectral microscopy for recording depth-resolved molecular maps of biological cells (Acanthamoeba castellanii trophozoites). Micrometric depth-discrimination and short acquisition times (20 minutes for single plane confocal image) was achieved.

Journal Article Type Article
Publication Date 2018-10
Journal Journal of Raman Spectroscopy
Print ISSN 0377-0486
Electronic ISSN 1097-4555
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 49
Issue 10
Pages 1660-1667
APA6 Citation Liao, Z., Sinjab, F., Elsheikha, H. M., & Notingher, I. (2018). Optical sectioning in multi-foci Raman hyperspectral imaging. Journal of Raman Spectroscopy, 49(10), 1660-1667. doi:10.1002/jrs.5450
DOI https://doi.org/10.1002/jrs.5450
Keywords Confocal Raman imaging; optical sectioning; multi-beam; cross-talk; single cell imaging
Publisher URL https://onlinelibrary.wiley.com/doi/full/10.1002/jrs.5450
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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