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Distinct branches of the N-end rule pathway modulate the plant immune response

Vicente, Jorge; Mendiondo, Guillermina M.; Pauwels, Jarne; Pastor, Victoria; Izquierdo, Yovanny; Naumann, Christin; Movahedi, Mahsa; Rooney, Daniel J.; Gibbs, Daniel J.; Smart, Katherine; Bachmair, Andreas; Gray, Julie E.; Dissmeyer, Nico; Castresana, Carmen; Ray, Rumiana V.; Gevaert, Kris; Holdsworth, Michael J.

Authors

Jarne Pauwels

Victoria Pastor

Yovanny Izquierdo

Christin Naumann

Mahsa Movahedi

Daniel J. Rooney

Daniel J. Gibbs

Katherine Smart

Andreas Bachmair

Julie E. Gray

Nico Dissmeyer

Carmen Castresana

Kris Gevaert



Abstract

© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust The N-end rule pathway is a highly conserved constituent of the ubiquitin proteasome system, yet little is known about its biological roles. Here we explored the role of the N-end rule pathway in the plant immune response. We investigated the genetic influences of components of the pathway and known protein substrates on physiological, biochemical and metabolic responses to pathogen infection. We show that the glutamine (Gln) deamidation and cysteine (Cys) oxidation branches are both components of the plant immune system, through the E3 ligase PROTEOLYSIS (PRT)6. In Arabidopsis thaliana Gln-specific amino-terminal (Nt)-amidase (NTAQ1) controls the expression of specific defence-response genes, activates the synthesis pathway for the phytoalexin camalexin and influences basal resistance to the hemibiotroph pathogen Pseudomonas syringae pv tomato (Pst). The Nt-Cys ETHYLENE RESPONSE FACTOR VII transcription factor substrates enhance pathogen-induced stomatal closure. Transgenic barley with reduced HvPRT6 expression showed enhanced resistance to Ps. japonica and Blumeria graminis f. sp. hordei, indicating a conserved role of the pathway. We propose that that separate branches of the N-end rule pathway act as distinct components of the plant immune response in flowering plants.

Citation

Vicente, J., Mendiondo, G. M., Pauwels, J., Pastor, V., Izquierdo, Y., Naumann, C., …Holdsworth, M. J. (2019). Distinct branches of the N-end rule pathway modulate the plant immune response. New Phytologist, 221(2), 988-1000. https://doi.org/10.1111/nph.15387

Journal Article Type Article
Acceptance Date Jul 11, 2018
Online Publication Date Aug 17, 2018
Publication Date Jan 1, 2019
Deposit Date Jul 12, 2018
Publicly Available Date Sep 7, 2018
Journal New Phytologist
Print ISSN 0028-646X
Electronic ISSN 1469-8137
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 221
Issue 2
Pages 988-1000
DOI https://doi.org/10.1111/nph.15387
Public URL http://eprints.nottingham.ac.uk/id/eprint/52925
Publisher URL https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.15387
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Distinct branches of the N-end rule pathway modulate the plant immune response (1.1 Mb)
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/

Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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