Maria E. Alea-Reyes
Synthesis and in vitro phototoxicity of multifunctional Zn(II)meso-tetrakis(4-carboxyphenyl)porphyrin-coated gold nanoparticles assembled via axial coordination with imidazole ligands
Alea-Reyes, Maria E.; Penon, Oriol; García Calavia, Paula; Marín, María J.; Russell, David A.; Pérez-García, Lluïsa
Authors
Oriol Penon
Paula García Calavia
María J. Marín
David A. Russell
Lluïsa Pérez-García
Abstract
Hypothesis
Metalloporphyrins are extensively investigated for their ability to form reactive oxygen species and as potent photosensitisers for use in photodynamic therapy. However, their hydrophobicity generally causes solubility issues concerning in vivo delivery due to lack of distribution and low clearance from the body. Immobilising porphyrins on carriers, such as gold nanoparticles (GNP), can overcome some of these drawbacks. The mode of assembling the porphyrins to the carrier influences the properties of the resulting drug delivery systems.
Experiments
We describe the synthesis and characterisation of new porphyrin decorated water soluble GNP and we explore Zn-imidazole axial coordination as the mode of linking the porphyrin to the metallic core of the nanoparticles. Quantification of singlet oxygen production, toxicity in dark, cellular uptake by SK-BR-3 cells and phototoxicity have been assessed.
Findings
Axial coordination limits the number of porphyrins on the gold surface, reduces the formation of aggregates, and diminishes metal exchange in the porphyrin, all of which contribute to enhance the efficiency of singlet oxygen generation from the immobilised porphyrin. In vitro experiments on SK-BR-3 cells reveal a fast uptake followed by more than 80% cell death after irradiation with low doses of light.
Citation
Alea-Reyes, M. E., Penon, O., García Calavia, P., Marín, M. J., Russell, D. A., & Pérez-García, L. (2018). Synthesis and in vitro phototoxicity of multifunctional Zn(II)meso-tetrakis(4-carboxyphenyl)porphyrin-coated gold nanoparticles assembled via axial coordination with imidazole ligands. Journal of Colloid and Interface Science, 521, https://doi.org/10.1016/j.jcis.2018.03.014
Journal Article Type | Article |
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Acceptance Date | Mar 6, 2018 |
Online Publication Date | Mar 7, 2018 |
Publication Date | Jul 1, 2018 |
Deposit Date | Apr 4, 2018 |
Publicly Available Date | Mar 8, 2019 |
Journal | Journal of Colloid and Interface Science |
Print ISSN | 0021-9797 |
Electronic ISSN | 1095-7103 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 521 |
DOI | https://doi.org/10.1016/j.jcis.2018.03.014 |
Keywords | Zn(II)meso-tetrakis(4-carboxyphenyl)porphyrin; Gold nanoparticles; Imidazole ligands Zn(II)-imidazole axial coordination; Singlet oxygen production; SK-BR-3 cells; Cellular uptake; Dark cytotoxicity; Phototoxiciy; Photodynamic therapy |
Public URL | https://nottingham-repository.worktribe.com/output/943088 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S002197971830256X |
Files
Revised Manuscript SM JCIS AuNPs-imidazole-porphyrin.pdf
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0
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