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Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support

Carucci, Cristina; Bruen, Larah; Gascón, Victoria; Paradisi, Francesca; Magner, Edmond

Authors

Cristina Carucci

Larah Bruen

Victoria Gascón

Francesca Paradisi

Edmond Magner



Abstract

The use of an in-situ immobilization procedure for the immobilization of hyperhalophilic alcohol dehydrogenase in a metal organic framework material is described. The easy and rapid in situ immobilization process enables retention of activity over a broad range of pH and temperature together with a decrease in the halophilicity of the enzyme. The catalytic activity of the immobilized enzyme was studied in non-aqueous solvent mixtures with the highest retention of activity in aqueous solutions of methanol and acetonitrile. The approach demonstrates that this immobilization method can be extended to hyperhalophilic enzymes with enhancements in activity and stability.

Journal Article Type Article
Journal Langmuir
Print ISSN 0743-7463
Electronic ISSN 0743-7463
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
APA6 Citation Carucci, C., Bruen, L., Gascón, V., Paradisi, F., & Magner, E. (in press). Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support. Langmuir, doi:10.1021/acs.langmuir.8b01037
DOI https://doi.org/10.1021/acs.langmuir.8b01037
Publisher URL https://pubs.acs.org/doi/abs/10.1021/acs.langmuir.8b01037
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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