Rapid formulation of redox-responsive oligo-?-aminoester polyplexes with siRNA via jet printing

Lovato, Tatiana; Taresco, Vincenzo; Alazzo, Ali; Sansone, Catarina; Stolnik, Snjezana; Alexander, Cameron; Conte, Claudia

Abstract

Here we describe a rapid inkjet formulation method for screening newly-synthesised cationic materials for siRNA delivery into cancer cells. Reduction responsive oligo-?-aminoesters were prepared and evaluated for their ability to condense siRNA into polyplexes through a fast inkjet printing method. A direct relationship between the oligomer structures and charge densities, and the final cell response in terms of uptake rate and transfection efficacy, was found. The oligo-?-aminoesters were well-tolerated by the cancer cells, compared to conventional cationic polymers so far employed in gene delivery, and were as active in silencing of a representative luciferase gene.

Citation

Lovato, T., Taresco, V., Alazzo, A., Sansone, C., Stolnik, S., Alexander, C., & Conte, C. (2018). Rapid formulation of redox-responsive oligo-?-aminoester polyplexes with siRNA via jet printing. Journal of Materials Chemistry B, 6(41), 6550-6558. https://doi.org/10.1039/C8TB01215F

• Journal Article Type: Article
• Acceptance Date: Jun 1, 2018
• Online Publication Date: Oct 9, 2018
• Publication Date: Oct 9, 2018
• Deposit Date: Jun 5, 2018
• Publicly Available Date: Oct 10, 2019
• Journal: Journal of Materials Chemistry B
• Print ISSN: 2050-750X
• Electronic ISSN: 2050-7518
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 41
• Pages: 6550-6558
• DOI: https://doi.org/10.1039/C8TB01215F
• Public URL: https://nottingham-repository.worktribe.com/output/935413
• Publisher URL: https://pubs.rsc.org/en/content/articlehtml/2018/tb/c8tb01215f