Skip to main content

Research Repository

Advanced Search

High-Throughput Miniaturized Screening of Nanoparticle Formation via Inkjet Printing

Styliari, Ioanna D.; Conte, Claudia; Pearce, Amanda K.; H�sler, Amanda; Cavanagh, Robert J.; Limo, Marion J.; Gordhan, Dipak; Nieto-Orellana, Alejandro; Suksiriworapong, Jiraphong; Couturaud, Benoit; Williams, Phil; Hook, Andrew L.; Alexander, Morgan R.; Garnett, Martin C.; Alexander, Cameron; Burley, Jonathan C.; Taresco, Vincenzo

High-Throughput Miniaturized Screening of Nanoparticle Formation via Inkjet Printing Thumbnail


Ioanna D. Styliari

Claudia Conte

Amanda K. Pearce

Amanda H�sler

Biophysical Analyst

Dipak Gordhan

Alejandro Nieto-Orellana

Jiraphong Suksiriworapong

Benoit Couturaud

Professor of Biophysics

Assistant Professor

Profile Image

Professor of Biomedical Surfaces

Martin C. Garnett


The self‐assembly of specific polymers into well‐defined nanoparticles (NPs) is of great interest to the pharmaceutical industry as the resultant materials can act as drug delivery vehicles. In this work, a high‐throughput method to screen the ability of polymers to self‐assemble into NPs using a picoliter inkjet printer is presented. By dispensing polymer solutions in dimethyl sulfoxide (DMSO) from the printer into the wells of a 96‐well plate, containing water as an antisolvent, 50 suspensions are screened for nanoparticle formation rapidly using only nanoliters to microliters. A variety of polymer classes are used and in situ characterization of the submicroliter nanosuspensions shows that the particle size distributions match those of nanoparticles made from bulk suspensions. Dispensing organic polymer solutions into well plates via the printer is thus shown to be a reproducible and fast method for screening nanoparticle formation which uses two to three orders of magnitude less material than conventional techniques. Finally, a pilot study for a high‐throughput pipeline of nanoparticle production, physical property characterization, and cytocompatibility demonstrates the feasibility of the printing approach for screening of nanodrug delivery formulations. Nanoparticles are produced in the well plates, characterized for size and evaluated for effects on metabolic activity of lung cancer cells.

Journal Article Type Article
Acceptance Date May 27, 2018
Online Publication Date May 27, 2018
Publication Date 2018-08
Deposit Date Jun 5, 2018
Publicly Available Date May 28, 2019
Journal Macromolecular Materials and Engineering
Print ISSN 1438-7492
Electronic ISSN 1439-2054
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 303
Issue 8
Article Number 1800146
Pages 1-9
Keywords High-throughput miniaturized screening; Inkjet printers; Nanoparticles; Self-assembling
Public URL
Publisher URL
Additional Information This is the accepted version of the following article: I. D. Styliari, C. Conte, A. K. Pearce, A. Hüsler, R. J. Cavanagh, M. J. Limo, D. Gordhan, A. Nieto-Orellana, J. Suksiriworapong, B. Couturaud, P. Williams, A. L. Hook, M. R. Alexander, M. C. Garnett, C. Alexander, J. C. Burley, V. Taresco, Macromol. Mater. Eng. 2018, 303, 1800146., which has been published in final form at This article may be used for non-commercial purposes in accordance with the Wiley Self-Archiving Policy.


You might also like

Downloadable Citations