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TYPLEX® Chelate, a novel feed additive, inhibits Campylobacter jejuni biofilm formation and cecal colonization in broiler chickens

Khattak, Farina; Green, M.; Paschalis, Vasileios; Houdijk, J.G.M.; Soultanas, P.; Mahdavi, J.

Authors

Farina Khattak

M. Green

Vasileios Paschalis

J.G.M. Houdijk

J. Mahdavi jafar.mahdavi@nottingham.ac.uk



Abstract

Reducing Campylobacter spp. carriage in poultry is challenging, but essential to control this major cause of human bacterial gastroenteritis worldwide. Although much is known about the mechanisms and route of Campylobacter spp. colonization in poultry the literature is scarce on antibiotic-free solutions to combat Campylobacter spp. colonization in poultry. In vitro and in vivo studies were conducted to investigate the role of TYPLEX® Chelate, a novel feed additive, in inhibiting Campylobacter jejuni (C. jejuni) biofilm formation and reducing C. jejuni and Escherichia coli (E. coli) colonization in broiler chickens at market age. In an in vitro study, the inhibitory effect on C. jejuni biofilm formation using a plastic bead assay was investigated. The results demonstrated that TYPLEX® Chelate significantly reduces biofilm formation. For in vivo study, 800 broilers (one-day old) were randomly allocated to 4 dietary treatments in a randomised block design, each having 10 replicate pens with 20 birds per pen. At day 21, all birds were challenged with C. jejuni via seeded litter. At day 42, caecal samples were collected and tested for volatile fatty acid (VFA) concentrations, C. jejuni and E. coli counts. The results showed that TYPLEX® Chelate reduced the carriage of C. jejuni and E. coli in poultry by 2 and 1 log₁₀ per gram caecal sample, respectively, and increased caecal VFA concentrations. These findings support TYPLEX® Chelate as a novel non-antibiotic feed additive that may help produce poultry with a lower public health risk of Campylobacteriosis.

Citation

Khattak, F., Green, M., Paschalis, V., Houdijk, J., Soultanas, P., & Mahdavi, J. (2018). TYPLEX® Chelate, a novel feed additive, inhibits Campylobacter jejuni biofilm formation and cecal colonization in broiler chickens. Poultry Science, 97(4), https://doi.org/10.3382/ps/pex413

Journal Article Type Article
Acceptance Date Nov 26, 2017
Online Publication Date Feb 15, 2018
Publication Date Apr 1, 2018
Deposit Date Nov 28, 2017
Publicly Available Date Feb 15, 2018
Journal Poultry Science
Print ISSN 0032-5791
Electronic ISSN 1525-3171
Publisher Oxford University Press
Peer Reviewed Peer Reviewed
Volume 97
Issue 4
DOI https://doi.org/10.3382/ps/pex413
Keywords Campylobacter, biofilm, volatile fatty acid, feed additive, food safety
Public URL http://eprints.nottingham.ac.uk/id/eprint/48398
Publisher URL https://academic.oup.com/ps/advance-article/doi/10.3382/ps/pex413/4864308
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0





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