Martin A. Bewley
Opsonic phagocytosis in chronic obstructive pulmonary disease is enhanced by Nrf2 agonists
Bewley, Martin A.; Budd, Richard C.; Ryan, Eilise; Cole, Joby; Collini, Paul; Marshall, Jennifer; Kolsum, Umme; Beech, Gussie; Emes, Richard D.; Tcherniaeva, Irina; Berbers, Gguy A.M.; Walmsley, Sarah R.; Donaldson, Gavin; Wedzicha, Jadwiga A.; Kilty, Iain; Rumsey, William; Sanchez, Yolanda; Brightling, Christopher E.; Donnelly, Louise E.; Barnes, Ppeter J.; Singh, Dave; Whyte, Moira K.B.; Dockrell, David H.
Authors
Richard C. Budd
Eilise Ryan
Joby Cole
Paul Collini
Jennifer Marshall
Umme Kolsum
Gussie Beech
Richard D. Emes
Irina Tcherniaeva
Gguy A.M. Berbers
Sarah R. Walmsley
Gavin Donaldson
Jadwiga A. Wedzicha
Iain Kilty
William Rumsey
Yolanda Sanchez
Christopher E. Brightling
Louise E. Donnelly
Ppeter J. Barnes
Dave Singh
Moira K.B. Whyte
David H. Dockrell
Abstract
Rationale: Previous studies have identified defects in bacterial phagocytosis by alveolar macrophages (AM) in patients with chronic obstructive pulmonary disease (COPD) but the mechanisms and clinical consequences remain incompletely defined.
Objectives: To examine the effect of COPD on AM phagocytic responses and identify the mechanisms, clinical consequences and potential for therapeutic manipulation of these defects.
Methods: We isolated alveolar macrophages (AM) and monocyte-derived macrophages (MDM) from a cohort of COPD patients and controls within the MRC COPD-MAP consortium and measured phagocytosis of bacteria in relation to opsonic conditions and clinical features.
Measurements and Main Results: COPD AM and MDM have impaired phagocytosis of S. pneumoniae. COPD AM have a selective defect in uptake of opsonized bacteria, despite the presence of anti-pneumococcal antibodies in bronchoalveolar lavage, not observed in MDM or healthy donor’s AM. AM defects in phagocytosis in COPD are significantly associated with exacerbation frequency, isolation of pathogenic bacteria and health related quality of life scores. Bacterial binding and initial intracellular killing of opsonized bacteria in COPD AM was not reduced. COPD AM have reduced transcriptional responses to opsonized bacteria, including cellular stress responses that include transcriptional modules involving antioxidant defenses and Nrf2-regualted genes. Agonists of the cytoprotective transcription factor Nrf2 (sulforaphane and Compound) reverse defects in phagocytosis of S. pneumoniae and non-type able Haemophilus influenzae by COPD.
Conclusions: Patients with COPD have clinically relevant defects in opsonic phagocytosis by AM, associated with impaired transcriptional responses to cellular stress, which are reversed by therapeutic targeting with Nrf2 agonists.
Citation
Bewley, M. A., Budd, R. C., Ryan, E., Cole, J., Collini, P., Marshall, J., …Dockrell, D. H. (in press). Opsonic phagocytosis in chronic obstructive pulmonary disease is enhanced by Nrf2 agonists. American Journal of Respiratory and Critical Care Medicine, https://doi.org/10.1164/rccm.201705-0903OC
Journal Article Type | Article |
---|---|
Acceptance Date | Mar 14, 2018 |
Online Publication Date | Mar 16, 2018 |
Deposit Date | Apr 20, 2018 |
Publicly Available Date | Mar 17, 2019 |
Journal | American Journal of Respiratory and Critical Care Medicine |
Print ISSN | 1073-449X |
Electronic ISSN | 1073-449X |
Publisher | American Thoracic Society |
Peer Reviewed | Peer Reviewed |
DOI | https://doi.org/10.1164/rccm.201705-0903OC |
Keywords | COPD ; Macrophage ; Phagocytosis ; Anti-oxidant ; Nrf2 |
Public URL | https://nottingham-repository.worktribe.com/output/920090 |
Publisher URL | https://www.atsjournals.org/doi/10.1164/rccm.201705-0903OC |
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